Estimating foliar anthocyanin content of purple corn via hyperspectral model

Abstract

To date, the foliar anthocyanin content was either determined via the pH differential or HPLC methods, both of which are slow and destructive. Here, a hyperspectral model was established to estimate the foliar anthocyanin content of purple corn (Zea mays L. var. Jingzi No. 1). The reflectivity (P) of the foliar hyperspectral was inverted to 1/P, lg P, 1/lg P, P′, 1/P′, lgP′, and 1/lgP′. The correlation coefficient between these inversions and the foliar anthocyanin content was plotted against the hyperspectral wavelength. The wavelength of inversions around 650 nm was sensitive to the foliar anthocyanin content. The hyperspectral model was fitted via linear, polynomial, power, exponential, and logarithmic functions with the sensitive band as independent variable and the anthocyanin content as function. The hyperspectral model (y = 3,000,000,000 × W 685 4.5896) fitted via inversion of lgP′ showed the highest determination coefficients (0.768) among all models. The hyperspectral model was well validated with a determination coefficient of 0.932 and an RMSE of 0.0065. Moreover, the accuracy and stability of the hyperspectral model were further enhanced with a determination coefficient of 0.954 and RMSE of 0.0047 when the anthocyanin content of the sample was below 20 mg/g. Hence, the hyperspectral model estimated the foliar anthocyanin content of purple corn quickly and nondestructively.

Comparing pH differential and methanol‐based methods for anthocyanin assessments of strawberries

“…The supernatant was then removed, and the absorbance was read at 520 and 700 nm. The 5 g frozen strawberry samples were then combined with 20 ml buffer pH 4.5 (0.4 M sodium acetate buffer) and incubated and centrifuged similarly (Gu et al, 2018). The absorbance of the supernatant was measured at the same wavelengths.…”

Measurement of Environmentally Influenced Variations in Anthocyanin Accumulations in Brassica rapa subsp. Chinensis (Bok Choy) Using Hyperspectral Imaging

“…There were various attempts to analyze anthocyanin accumulation non-destructively, such as the hyperspectral model (Gu et al, 2018 ), UV-Spec. (Gitelson et al, 2009 ), and the fluorimetric sensor (Tuccio et al, 2011 ).…”

Colored Corn: An Up-Date on Metabolites Extraction, Health Implication, and Potential Use

“…Its content in a new dark red corn variety was evaluated using a quick, simple and conservative method, namely hyperspectral model, generally used for measuring moisture, nitrogen, and chlorophyll content. To overcome the weak relationship between the anthocyanin content and the hyperspectrum, a sensitive band (685 nm) was selected via multiple linear regression thus enhancing the accuracy and the stability of the model, also when the anthocyanin content was lower than 20 mg/g [ 59 ].…”