Wheat production occupies an important place for ensuring human nutrition and there is great varietal potential to increase its productivity as the area under crops cannot be increased in several regions. It is wider adaptability as well as the quality of nutritive values than other cereals (Yassin et al., 2019). Hence, it has frequently emphasized the development of appropriate breeding techniques, especially the improvement of new, high yielding quality varieties which are indispensable for vertical expunction i.e., increase productivity (Pena et al., 2002; Tester and Langridge, 2010; Kizilgeci et al., 2019a). It is widely grown food cereal worldwide, due to its wider adaptability as well as the quality of nutritional values than other cereals. Furthermore, it is as a strategic crop that plays a key role in the national economy for several countries (Yildirim et al., 2018; Kizilgeci et al., 2019b). Its demand is increasing day by day to meet the food security of an increasing population (Otu Borlu et al., 2018; Khaled et al., 2018). Nitrogen requirement of wheat is higher owing to its role in vegetative growth and generative development (Van Keulen and Seligman, 1987; Frederick and Camberato, 1995; Kizilgeci et al., 2016). It has been reported that there is a significant association between nitrogen rates and yield components as well as the yield of wheat (Colkesen et al., 1993) and it is observed that the yield was increased in wheat more than 50% due to nitrogen application (Karaca et al., 1993). This effect is determined by the stay-green period of the spike and flag leaf (Quanyi et al., 2007). Especially in arid climates, photosynthesis in the spike provides important aids to the dry matter which contributes filling of grain (Tambussi et al., 2007). Although the yield and quality traits of wheat largely depend on the optimum nitrogen level (Dogan et al., 2008; Iqbal et al., 2012; Aydogan Cifci and Dogan, 2013) and genotypic variation (Barbottin et al., 2005). The significant in genetic improvement of nitrogen use efficiency was considered in different studies at various N levels. Ortiz-Monasterio et al. (1997) noted nitrogen use efficiency (NUE) genetic progression of 0.4-1.1 % per year depending on the N levels in spring CIMMYT wheat varieties between 1962 and 1985. Cormier et al. (2014) estimated genetic progress of 0.30-0.37% per year between 1985 and 2010 using 195 European elite winter varieties at optimal and suboptimal N levels. Due to the law of diminishing marginal utility, the nitrogen rates beyond a threshold does not provide improves in yield but increases the production costs and leads to environmental pollution. For that reason, the development of wheat varieties with high NUE considered an essential target for the researchers. Using SPAD readings to evaluate leaf chlorophyll