ObjectivesBurial space reuse and prolonged interaction with the dead were common practices in the Isthmo‐Colombian Area, dating back to at least the Early Ceramic Period in the Greater Coclé region. However, biological and social relationships of individuals interred in collective burial contexts remain unclear. Here, we explore intra‐cemetery biological variation through a biological distance analysis of individuals interred in large mortuary features from the first mortuary horizon at the site of Cerro Juan Díaz in Panamá.Materials and MethodsIndividuals recovered from Features 2, 16, and 94 from Operation 3 at Cerro Juan Díaz were assessed for dental metric and nonmetric traits. Biological distances were calculated through Gower coefficients and subsequently assessed visually with PCoA plots. Differences in phenotypic variability within burial feature (n = 26), age (postpubescent individuals aged 15+ years vs. prepubescent individuals; n = 27), and sex (n = 14) groupings were evaluated statistically via permutational multivariate analysis of variance (PERMANOVA) and PERMDISP tests.ResultsThe estimated distances for each individual showed broad similarities between the three burial features. Significant differences in biological distance were found only when comparing by age. Gower coefficients for prepubescent individuals differed from those of postpubescent individuals in terms of both group centroid location and data point dispersion around centroids.DiscussionOur results are largely consistent with previous notions of Cerro Juan Díaz's function as a community burial ground. Prepubescent individuals may have been brought to the site for burial from nearby villages, perhaps following specific mortuary traditions governed by age group.