The ecology of the southern flying squirrel, Glaucomys volans (L i nn a e u s, 1758) was studied in 2 areas of central Virginia, using artificial tree shelter traps and baited live trapping. Within one year after their installation, 46.9% of the artificial shelters were used for nesting sites, 26.6% as feeding stations, and 17.2% as defecatoria; only 9.4% were without evidence of use. The animals utilized several shelters for nesting in addition to others used for food storage and defecatoria. In habitat selection, availability of bodies of fresh water was important, but the slope of the terrain was not significant. Foraging (average range 126.8 m ± 14.8 S.E.) was not significantly related to distance from aquatic habitat. Adult males foraged substantially farther than sexually inactive females or juveniles. Most females (94.2%) became pregnant within 6-8 months after birth. Birth of young occurred in early spring, March and April, and in late summer, from August to early October. Removal rate of young squirrels from the population by mortality was 50% within 5.5 months and 67% in 7 months. Population density, estimated by the Petersen index and regression analysis of recapture frequency, varied at different seasons from 4.5 to 10.1 flying squirrels/ha at one of the localities, and from 6.2 to 13.8/ha at the other.