Managing nitrogen (N) inputs to sustain high yields while minimizing losses to adjacent environments remains among the foremost aims in agroecosystems. We studied the N balance in a study established in 1911 at Lethbridge, AB, Canada. The experiment includes three cropping systemscontinuous wheat (W), fallowwheat-wheat (FWW), and fallow-wheat (FW)with a factorial of two N rates (0 and 45 kg N ha −1) and two phosphorus (P) rates (0 and 20 kg P ha −1) superimposed beginning in 1967. In unfertilized subplots, grain yields generally increased for the first eight decades, but then declined, perhaps partly because of growing N deficiency. Yield response to N increased over time, especially under continuous cropping and when co-applied with P. Soil N concentration in the surface 15 cm declined in the first few decades, and then approached an apparent steady state. Application of N increased soil N, roughly in proportion to the amount of residue returned. For the first half-century (1911-1967), N removal was approximately equivalent to the loss of soil N in the surface 15 cm. Since then, however, when the soil organic N was near steady state, removals of N in grain exceeded N inputs by approximately 20-30 kg N ha −1 yr −1 , suggesting an input from outside sources, perhaps partly from atmospheric NH 3. This study demonstrated the importance of long-term experiments in evaluating the N balance of cropping systems, and indicated the potential significance of non-fertilizer N inputs from outside sources in such ecosystems.