Estimating the Toxicity and Biological Availability for Interaction Products of Metallic Iron and Humic Substances

Anuchina, M. M.; Pankratov, D. А.; Abroskin, Dmitry P.; Куликова, Н. А.; Gabbasova, D. T.; Маторин, Д. Н.; Volkov, Dmitry S.; Perminova, Irina V.

doi:10.3103/s0147687419050028

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…Another important application is the removal of ecotoxicants from natural waters and industrial wastes [ 1 , 15 ]. Therefore, iron oxide nanomaterials can be found in natural and engineering aquatic environments due to their numerous applications, as well as corrosion products of metal components in the presence of natural materials [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Functionalized Magnetite Nanoparticles: Characterization, Bioeffects, and Role of Reactive Oxygen Species in Unicellular and Enzymatic Systems

Kicheeva

Sushko

Bondarenko

et al. 2023

IJMS

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The current study evaluates the role of reactive oxygen species (ROS) in bioeffects of magnetite nanoparticles (MNPs), such as bare (Fe3O4), humic acids (Fe3O4-HA), and 3-aminopropyltriethoxysilane (Fe3O4-APTES) modified MNPs. Mössbauer spectroscopy was used to identify the local surrounding for Fe atom/ions and the depth of modification for MNPs. It was found that the Fe3O4-HA MNPs contain the smallest, whereas the Fe3O4-APTES MNPs contain the largest amount of Fe2+ ions. Bioluminescent cellular and enzymatic assays were applied to monitor the toxicity and anti-(pro-)oxidant activity of MNPs. The contents of ROS were determined by a chemiluminescence luminol assay evaluating the correlations with toxicity/anti-(pro-)oxidant coefficients. Toxic effects of modified MNPs were found at higher concentrations (>10−2 g/L); they were related to ROS storage in bacterial suspensions. MNPs stimulated ROS production by the bacteria in a wide concentration range (10−15–1 g/L). Under the conditions of model oxidative stress and higher concentrations of MNPs (>10−4 g/L), the bacterial bioassay revealed prooxidant activity of all three MNP types, with corresponding decay of ROS content. Bioluminescence enzymatic assay did not show any sensitivity to MNPs, with negligible change in ROS content. The results clearly indicate that cell-membrane processes are responsible for the bioeffects and bacterial ROS generation, confirming the ferroptosis phenomenon based on iron-initiated cell-membrane lipid peroxidation.

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Section: Introductionmentioning

confidence: 99%

Functionalized Magnetite Nanoparticles: Characterization, Bioeffects, and Role of Reactive Oxygen Species in Unicellular and Enzymatic Systems

Kicheeva

Sushko

Bondarenko

et al. 2023

IJMS

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ICP-OES Analysis for Humic Substance

Kolmykov

2023

Russ. Agricult. Sci.

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ICP-OES-Analysis for Humic Substance

Kolmykov

2023

Agrohimiâ

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The possibilities of optical emission spectrometry with inductively coupled plasma (OES-ICP analysis) (in radial plasma observation) of humic preparations (HP) are evaluated, the proposed technique allows quantifying up to 24 elements (As, Al, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, P, Pb, Si, S, Sr, Ti, V and Zn). The list of these elements will allow determining the value of such preparations for soil nutrition, and will also help to avoid danger if the content of toxic impurities in them goes beyond their maximum permissible concentrations. In the absence of standard samples with certified contents of the elements to be determined, the correctness of the analysis for the macrocomponent composition is confirmed by comparing the results of gravimetric determination of the ash content of the studied objects with the calculated data obtained when processing the results of OES-ICP analysis. Systematic errors in the determination of trace impurities were minimized by using the method of adding standard solutions (from matrix influences), as well as 6 parallel measurements of samples prepared independently of each other. The correctness of the methodology was also evaluated by using model solutions close to the possible micro-impurity mineral composition of HP using the Student’s t-test. The results obtained for the isolated fraction of humic acids are compared with the literature data.

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Estimating the Toxicity and Biological Availability for Interaction Products of Metallic Iron and Humic Substances

Cited by 4 publications

References 14 publications

Functionalized Magnetite Nanoparticles: Characterization, Bioeffects, and Role of Reactive Oxygen Species in Unicellular and Enzymatic Systems

Functionalized Magnetite Nanoparticles: Characterization, Bioeffects, and Role of Reactive Oxygen Species in Unicellular and Enzymatic Systems

ICP-OES Analysis for Humic Substance

ICP-OES-Analysis for Humic Substance

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