Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles

Dion, Marie-Lise; Sékaly, Rafick‐Pierre; Cheynier, Rémi

doi:10.1007/978-1-59745-395-0_12

Cited by 78 publications

(66 citation statements)

References 18 publications

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“…This shift is thought to mainly result from thymic involution, which is the shrinking in size and function of the thymus that leads to the reduced output of new naïve T cells toward the periphery [16]. Measurement of T-cell receptor excision circle molecules (TREC) expression has been shown to be a reliable marker to assess thymus function [17]. By assessing TREC expression on human peripheral blood mononuclear cells, Sempowski and co-workers showed that the number of naïve T cells drops by 3 orders of magnitude over an 80 year lifespan [18].…”

Section: Age-associated Quantitative and Qualitative Changes In The Cmentioning

confidence: 99%

Aging of the CD4 T Cell Compartment

Lefebvre

Haynes

2012

TOLSJ

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Higher morbidity and mortality following infections, particularly influenza, is observed in the elderly population. Because of this, people over 65 years old are often targeted for preventive immunization. Many vaccines, however, are not as effective in generating protective antibodies in older individuals. CD4+ T cells, through their B cell helper functions, play a central role in the humoral response. Aging has deleterious effects on the immune system, and understanding how aging impairs CD4+ T cell functions is of critical importance to design new immunization and treatment strategies targeted to the elderly population. In this paper, we review some of the qualitative and quantitative changes in the CD4+ T cell compartment that arise with aging. We also summarize the age-related intrinsic defects that impact naïve, memory and regulatory CD4+ T cell functions.

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Section: Age-associated Quantitative and Qualitative Changes In The Cmentioning

confidence: 99%

Aging of the CD4 T Cell Compartment

Lefebvre

Haynes

2012

TOLSJ

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“…sjTRECs are episomal DNA fragments resulting from the deletion of the T cell receptor d region during T cell receptor a locus rearrangement. Because they cannot replicate and are not duplicated, they are diluted out during cell division, allowing a direct evaluation of recent thymic output [8,9].…”

Section: Introductionmentioning

confidence: 99%

Association between Thymic Function and Allogeneic Hematopoietic Stem Cell Transplantation Outcome: Results of a Pediatric Study

Saglio

Cena

Berger

et al. 2015

Biology of Blood and Marrow Transplantation

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Robust T cell function recovery has been shown to be crucial in determining allogeneic hematopoietic stem cell transplantation (HSCT) outcome, and there is growing evidence that the thymus plays a central role in regulating this process. We performed a long-term analysis of the role of thymic activity recovery in a population of pediatric patients undergoing allogeneic HSCT by signal joint T cell receptor excision circle (sjTREC) quantification. In this study, characterized by a long-term follow-up (median, 72 months), we found patients with higher levels of sjTRECs before transplantation had a statistically significant reduced risk of death compared with patients with lower values (relative risk, .31; 95% confidence interval, .30 to .32; P = .02), showing this different outcome was mainly related to a reduction of relapse incidence (14% versus 43%, P = .02). Unlike previous reports, we observed no correlation between sjTREC levels and lymphocyte recovery. Moreover, we confirmed that only graft-versus-host disease influenced thymic activity after transplantation. In conclusion, our results suggest an association between pretransplantation thymic activity and the long-term outcome of pediatric patients undergoing HSCT, mainly through a reduction of relapse opportunities.

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“…The intracellular TRECs are diluted as the cell proliferates, such that naïve T cells present with relatively high TREC levels, whereas antigen-experienced (memory) T cells have low TREC levels [47]. In the setting of PIDs, TREC evaluation can be used as a diagnostic confirmation of low Fig.…”

Section: T-cell Receptor Excision Circle Quantificationmentioning

confidence: 99%

Molecular- and Flow Cytometry-based Diagnosis of Primary Immunodeficiency Disorders

Oliveira

Fleisher

2010

Curr Allergy Asthma Rep

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Primary immunodeficiencies are an expanding group of genetic disorders resulting in recurrent and/or severe infections, autoimmunity, or autoinflammation. The laboratory plays a critical role in the diagnosis of these conditions given their frequently overlapping signs and symptoms. We discuss here advances in flow cytometry and molecular techniques applied to the study of primary immunodeficiencies.

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Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles

Cited by 78 publications

References 18 publications

Aging of the CD4 T Cell Compartment

Aging of the CD4 T Cell Compartment

Association between Thymic Function and Allogeneic Hematopoietic Stem Cell Transplantation Outcome: Results of a Pediatric Study

Molecular- and Flow Cytometry-based Diagnosis of Primary Immunodeficiency Disorders

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