Spatial and temporal variability of soil water extraction from the root zone affect soil water balance determination. The number of sensors installed in the root zone in studies addressing water balance is still set arbitrarily. This study provided an investigation of the water extraction process by banana (Musa spp.) roots by (i) determining the variability of water extraction from the banana tree root zone, (ii) detecting differences in the estimation of evapotranspiration (ET) by the soil water balance method when the number of soil profiles monitored in the roots zone varies, (iii) and; determining the minimum number of Time Domain Reflectometry (TDR) probes needed to obtain ET precision and accuracy similar to that determined by a drainage lysimeter. The field experiment was conducted in Cruz das Almas, in the state of Bahia, Brazil, where a drainage lysimeter was installed on a banana plantation. The water extraction in the banana root zone was quantified by the water content variations monitored in 72 points by TDRs, with measurements at 15-min intervals. The variability of water extraction in the banana root zone was medium to high. The range of variability affects the reliability of the crop evapotranspiration calculation by the soil water balance method. To prevent an overestimation of banana evapotranspiration, the water extraction in the soil profile must be monitored with at least 16 TDR probes installed at a minimum distance of 0.9 m and a minimum depth of 0.7 m.