Estimation In Vivo of the Antioxidant Activity of Axillary-Blueberry (Vaccinium axillare Nakai) Fruit under Oxidative Stress

Abstract

The intensity of free radical oxidation processes in vivo (a model of induced oxidative stress) was studied after a probe administration of the fruit extract of the axillary blueberry ( Vaccinium axillare Nakai). \ Four groups ( n = 40) of white CBA line male mice weighing 20–25 g were involved in the experiment: (1) intact control; (2) introduction of a 0.9% sodium-chloride solution orally for 10 days, a dose of 10 mL/kg per day; (3) “cisplatin” group (animals received 0.9% sodium-chloride solution similarly to group 2; on the fifth day of the experiment, cisplatin was administered one time by intraperitoneal injection at a dose of 7.5 mg/kg); and (4) “cisplatin + blueberry” group (mice received orally an extract of axillary-blueberry fruits at a dose of 10 mL/kg per day for 10 days; on the fifth day of the experiment, cisplatin was administered one time by intraperitoneal injection at a dose of 7.5 mg/kg). The antioxidant activity of the axillary blueberry was studied by a method of chemiluminescence. The analysis of kinetic parameters of chemiluminescence of mouse-kidney homogenate demonstrated that, against the background of a single intraperitoneal injection of cisplatin, oxidative stress develops in animals, with its severity decreasing under the action of axillary blueberry-fruit extract. The axillary blueberry-fruit extract has pronounced antioxidant properties and can play a role in the treatment and prevention of disease associated with oxidative stress.