Estimation of Accumulated Degree Day Value of Six Landmarks within the Pupal Stage of Lucilia sericata

Grisendi, Annalisa; Defilippo, Francesco; Gatti, Fábio; Dottori, M.; Bonilauri, Paolo

doi:10.17265/1934-7391/2015.07.003

Cited by 1 publication

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These studies frequently resulted in a series of images of the intra-puparial specimens, imaged from one or more orientation (most often dorsally), to show the morphological and colour changes that accompany metamorphosis. Examples of these, with varying degrees of detail and time resolution but all focussing entirely on external morphology of the intra-puparial specimens, have provided data for: Calliphora vicina [36], Lucilia sericata [37,38], Lucilia cuprina [39], Lucilia illustris [40], Chrysomya albiceps [41,42], Chrysomya megacephala [43], Chrysomya putoria [44] and Cochliomyia macellaria [39]. Similar studies have been undertaken on the forensically relevant scuttle flies, Megaselia spiracularis and Megaselia scalaris (Diptera: Phoridae) [45,46], and on the soldier fly, Hermetia illuscens (Diptera: Stratiomyidae) [47,48].…”

Section: Techniques (A) Light Microscopymentioning

confidence: 99%

Visualization of insect metamorphosis

Hall

Martín‐Vega

2019

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

Metamorphosis and, in particular, holometaboly, the development of organisms through a series of discrete stages (egg, larva, pupa, adult) that hardly resemble one another but are finely adapted to specific roles in the life cycle of the organism, has fascinated and mystified humans throughout history. However, it can be difficult to visualize the dramatic changes that occur during holometaboly without destructive sampling, traditionally through histology. However, advances in imaging technologies developed mainly for medical sciences have been applied to studies of insect metamorphosis over the past couple of decades. These include micro-computed tomography, magnetic resonance imaging and optical coherence tomography. A major advantage of these techniques is that they are rapid and non-destructive, enabling virtual dissection of an organism in any plane by anyone who has access to the image files and the necessary software. They can also be applied in some cases to visualize metamorphosis in vivo , including the periods of most rapid and dramatic morphological change. This review focusses on visualizing the intra-puparial holometabolous metamorphosis of cyclorraphous flies (Diptera), including the primary model organism for all genetic investigations, Drosophila melanogaster , and the blow flies of medical, veterinary and forensic importance, but also discusses similar studies on other insect orders. This article is part of the theme issue ‘The evolution of complete metamorphosis’.

show abstract