HIS RESEARCH work was conducted at Shandaweel Research …. Station, Sohag, Egypt, to study the combing ability and heterosis for yield and its attributes via Line × Tester analysis in sesame. Line × Tester mating design was applied in 2012 season using seven lines and four testers. In the two summers season 2013 and 2014, twentyeight F 1 hybrids, their parents and one commercial variety (Shandaweel 3) as a check were evaluated. Mean squares due to genotypes and years via combined analysis for the two years were highly significant for all studied traits, except each of 1000-seed weight and oil percentage across years. Also, mean squares due to the interaction between genotypes × years were highly significant for all the studied traits, except days to 50% flowering, branches plant -1 and 1000-seed weight. The analysis revealed that high significant differences were found among crosses and lines for all the studied traits over the two years. Likewise, it appeared high significant differences among the four testers for all traits, except for 1000-seed weight. High significant variances were detected for line × tester interaction over years for all studied traits. In this context, results showed that significant and highly significant differences were found for all the studied traits for the interactions; crosses × year (except for length of fruiting zone, branches plant -1 and1000-seed weight), tester × year (except for height of the first capsule, number of branches plant -1 and 1000-seed weight) and parents × crosses × year (except for days to 50% flowering, number of branches plant -1 , capsule length, 1000-seed weight and oil percentage). In general, the four