Estimation of Copper and Iron Burden in Biological Samples of Various Stages of Hepatitis C and Liver Cirrhosis Patients

Arain, Salma Aslam; Kazi, Tasneem Gul; Afridi, Hassan Imran; Talpur, Farah Naz; Mughal, Moina Akhtar; Shah, Faheem; Arain, Sadaf Sadia; Panhwar, Abdul Haleem

doi:10.1007/s12011-014-0058-9

Cited by 28 publications

(19 citation statements)

References 52 publications

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“…37 The digestion asks were cooled and the resulting solutions were evaporated to semidried mass. Triplicate serum samples of each patient and referent (0.2 mL), and replicate six samples of certied materials (serum) were directly taken into PTFE asks.…”

Section: Sample Preparationmentioning

confidence: 99%

Determination of trace levels of iron in serum samples of hepatitis B and C patients using dispersive liquid–liquid microextraction

et al. 2015

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An environmentally friendly ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLmE) method was developed for the preconcentration of trace levels of iron (Fe) in serum samples of hepatitis B and C patients, prior to its determination by flame atomic absorption spectrometry. The procedure was based on complexation of iron with a chelating reagent, 8-hydroxyquinoline (oxine), whereas an ionic liquid, 1butyl-3-methylimidazolium hexafluorophosphate [C 4 MIM][PF 6 ], and ethanol were chosen as extracting and dispersive solvents, respectively. The factors affecting the efficiency of IL-DLLmE, such as the amount of IL, pH, centrifugation time and ligand concentration, were investigated in detail. The resulting data indicated that the coexisting cations and anions ions had no apparent influence on determination ofFe at trace levels. Under optimal experimental conditions, the enhancement factor and the limit of detection were 59.1 and 1.29 mg L À1 , respectively. The relative standard deviation for five replicate determinations of Fe at 50 mg L À1 was calculated to be <5.0%. The developed method was validated by determining Fe in a certified reference material of serum. The proposed method can be successfully used for the enrichment of Fe in acid digested serum samples of female hepatitis B and C patients.

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Section: Sample Preparationmentioning

confidence: 99%

Determination of trace levels of iron in serum samples of hepatitis B and C patients using dispersive liquid–liquid microextraction

et al. 2015

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“…. 7 Under normal conditions, the liver takes up the Cu present in the circulation, Cu enters the hepatocytes and is subsequently distributed by Cu chaperones for its incorporation into cytochrome c oxidase, Cu-transporting P-type ATPase (ATP7B) and Cu/Zn superoxide dismutase (SOD). collaborate with these stellate cells.…”

Section: Introductionmentioning

confidence: 99%

Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry: a new approach for the diagnosis and prognosis of liver cirrhosis?

et al. 2015

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The isotopic composition of blood serum Cu has been investigated as a potential parameter for the diagnosis and prognosis of liver cirrhosis. Serum samples from supposedly healthy women (reference population) and from a group of female patients suffering from liver cirrhosis of different etiologies were analysed. The procedure for isolation of serum Cu and the measurement protocol for its isotopic analysis by multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS) were evaluated. Significant differences in the isotopic composition of Cu were observed between the reference population and the patients. A wide spread in δ(65)Cu was observed within the cirrhosis population and δ(65)Cu seems to be linked to the severity of the disease. Patients with end-stage liver disease showed a significantly lighter serum Cu isotopic composition. Many clinical parameters used for the diagnosis and monitoring of liver diseases, i.e. the levels of aspartate aminotransferase, De Ritis ratio, prothrombin and international normalized ratio, albumin, bilirubin, Na and C-reactive protein, correlate well with the δ(65)Cu values, as did the ceruloplasmin level and the ceruloplasmin/Cu concentration ratio. The isotopic composition of serum Cu appears to reveal the synthetic and hepatocellular function of the liver synergistically with inflammation and fluid retention in the cohort studied. A relevant relationship was also observed between δ(65)Cu and scores of mortality risk, such as the Model for End-stage Liver Disease (MELD) and MELD-Na. Thus, the isotopic composition of serum Cu shows potential as a new approach for the prognosis of liver disease, and although further investigation is required, for evaluation of the mortality risk in end-stage liver disease and prioritization of liver transplants.

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“…The Fe was first suggested as potentially important in the pathogenesis of HCV infection in 1992, with the demonstration of elevated Fe indices in many patients with chronic hepatitis C [23]. Normal Fe metabolism in the liver may be disrupted by viral hepatitis, and the accumulation of Fe in the liver is increased [24].…”

Section: Discussionmentioning

confidence: 99%

Evaluates the Efficiency of Interferon-Alpha Therapy for Different Time Intervals on the Levels of Iron in Serum Samples of Chronic Hepatitis C Patients

Kazi¹,

Arain²,

Afridi³

2016

Pharm Anal Acta

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It was investigated that the Interferon-α (IFN-α) therapy to patients with hepatitis C are correlated with iron levels in serum. The aim and objective of this study was to test whether the iron levels in blood serum samples were decreased after IFN-α therapy for different time intervals.In present follow-up study, iron levels in serum samples of chronic Hepatitis C (HCV) patients before and after IFN-α therapy at different time interval (24-48 weeks) were evaluated. 160 females HCV patients were selected for follow-up study. Blood samples were collected from patients before and after treatment of IFN-α therapy. For comparative purpose, blood samples from healthy female subjects of the same age group were also collected. The serum iron was determined by electro thermal atomic absorption spectrometry, after microwave assisted acid digestion method. After INF-α therapy, HCV RNA level was undetectable in 32% and 58% patients, for 24-48 weeks, respectively. The level of Fe in serum samples were significantly reduced after longer period of treatment with INF-α (48 weeks).

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Estimation of Copper and Iron Burden in Biological Samples of Various Stages of Hepatitis C and Liver Cirrhosis Patients

Cited by 28 publications

References 52 publications

Determination of trace levels of iron in serum samples of hepatitis B and C patients using dispersive liquid–liquid microextraction

Determination of trace levels of iron in serum samples of hepatitis B and C patients using dispersive liquid–liquid microextraction

Isotopic analysis of Cu in blood serum by multi-collector ICP-mass spectrometry: a new approach for the diagnosis and prognosis of liver cirrhosis?

Evaluates the Efficiency of Interferon-Alpha Therapy for Different Time Intervals on the Levels of Iron in Serum Samples of Chronic Hepatitis C Patients

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