Estimation of ferulic acid from selected plant materials by Spectrophotometry and High performance liquid chromatography

Abstract

Ferulic acid is an abundant phytophenolic compound present in plant cell wall. Ferulic acid possess anticancer, antioxidant, and anti-aging properties. A simple, sensitive and reproducible spectrophotometric method has been developed for quantitative estimation of ferulic acid from selected plant materials such as rice bran, wheat bran and bamboo shoot. The blue coloured chromogen obtained after the reaction was measured at wavelength of 718 nm for ferulic acid against the blank reagent. The chromogen obeyed linearity over the range of 1?g/ml - 8?g/ml. An HPLC method was also developed for the estimation of ferulic acid from selected plant materials.