Estimation of GCF Alkaline Phosphatase Levels in Health and Periodontal Disease – A Clinico Biochemical Study

Abstract

Introduction: Periodontitis is an inflammatory disease that is a common cause for damage to gingiva and periodontal tissue and for the loss of teeth. Tooth mobility, pathological migration and alveolar bone loss are common sequelae of periodontitis. Two types of periodontitis known are Chronic and Aggressive type. In gingival crevicular fluid (GCF), the activity of Alkaline phosphatase has been considered as a useful diagnostic and prognostic marker of periodontal disease. Based on this observation, a study has been conducted in patients with chronic and aggressive periodontitis and their age and gender matched healthy controls to assess the ALP levels in GCF. Material and methods: GCF alkaline phosphatase activity was observed in patients with aggressive and chronic periodontitis and their age and gender matched healthy controls. Results: On Comparison between GCF alkaline phosphatase levels between both aggressive and chronic periodontitis and their age and gender matched controls, the mean difference was statistically significant (P < 0.01) when paired t test was used. Conclusion: In patients with aggressive and chronic periodontitis, the mean alkaline phosphatase levels were increased significantly in comparison to healthy controls.

The Effect of Giving Aloe Vera Gel on Rats With Bacteria-Induced Periodontitis

“…Selain itu leukosit polimorfonuklear, bakteri dalam dental plak, osteoblas dan fibroblas adalah sumber utama ALP. 3 Rasio ALP pada GCF terhadap serum berkisar antara 6:1 hingga 11:1, hal ini menunjukkan bahwa sintesis alkaline phosphatase oleh enzim lebih banyak diproduksi secara lokal.…”

“…Rasio tersebut tidak ada hubungan yang signifikan antara total GCF-ALP dengan kadar ALP-plak dan analisis plak. 3 Hal ini menunjukkan kadar ALP yang normal berasal dari jaringan periodontal, maka peningkatan ALP pada penyakit periodontal menunjukkan reaksi metabolisme enzim terhadap perubahan jaringan yang disebabkan oleh inflamasi. 14 Aktivitas ALP dapat diamati sebaik mungkin sebagai indikator inflamasi gingiva, metabolisme tulang dan jumlah enzim sangat meningkat ketika terjadi inflamasi.…”