Estimation of genetic diversity and population structure in Tinospora cordifolia using SSR markers

Lade, Suchita; Pande, Veena; Rana, T. S.; Yadav, Hemant Kumar

doi:10.1007/s13205-020-02300-7

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…As an important indicator for evaluating the ability of species to adapt to environmental changes, genetic diversity is affected by many factors, such as natural selection, species migration, breeding systems, and genetic drift, which can provide a theoretical basis for the protection and utilization of Vicia resources (Lade et al, 2020). El‐Esawi (2017) used SSRs to evaluate the diversity and structure of 35 fava bean genotypes, the results show that He of SSR loci ranges between 0.51 and 0.81, and it is higher than the observed value (Abdolkarim et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity and population structure of the genus Vicia (Vicia L.) using simple sequence repeat markers

Sun

Cong

et al. 2022

Grassland Science

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The genetic diversity of the Vicia (Vicia L.) genus was analyzed using simple sequence repeat (SSR) markers. A total of 201 sampled individuals from 12 Vicia were assayed to study the genetic diversity and polymorphisms using 12 simple sequence repeat markers; 115 alleles were identified. The average observed heterozygosity (Ho) and the average expected heterozygosity (He) were 0.4283 and 0.6941, respectively. The polymorphic information content (PIC) varied from 0.8739 (V81) to 0.9579 (V97), with a mean of 0.9033, indicating that the markers were highly informative. Moreover, cluster and principal coordinate analysis (PCoA) divided the 12 species into three main clusters.Structure analysis and PCoA of Vicia narbonensis and Vicia tibetica agreed well with the cluster analysis results, and analysis of molecular variance (AMOVA) results revealed that 89% genetic variation was observed within the population and 11% was among the population. These results clarify the genetic diversity and population structure of the Vicia, providing useful information for understanding genetic variability and establishing a foundation for future breeding programs and genetic improvement.

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Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity and population structure of the genus Vicia (Vicia L.) using simple sequence repeat markers

Sun

Cong

et al. 2022

Grassland Science

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“…Journal of Nepal Biotechnology Association, 4 (1), 2023: 27-36 cordifolia exhibited the same banding patterns as that of mother plants which produced 22 bands of approximately 100-1400 bp confirming that no genetic variation occurred in the DNA of in vitro regenerated plantlets (Figure 4, 5). Contrarily, the ISSR markers revealed higher heterozygosity and thus can be attributed to the detection of high polymorphism which makes them the most preferred DNA marker (Lade et al, 2020).…”

Section: Rapd and Issr Analysismentioning

confidence: 99%

In vitro Propagation and Assessment of Genetic Homogeneity using RAPD and ISSR Markers in Tinospora cordifolia (Wild.) Hook. F. & Thoms, An Important Medicinal Plant of Nepal

Pandey

Maharjan

Pant

2023

J. Nepal. Biotech. Assoc.

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The Menispermaceae family includes the widely glabrous, succulent, climbing shrub Tinospora cordifolia (Gurjo), which has been found to have a variety of pharmacological and ethnomedicinal characteristics. T. cordifolia is also one of the most commercially exploited plants in pharmaceuticals. The nodal segment explants were initially cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzyl amino purine (BAP), and/or Kinetin (KIN) among which MS +BAP (2.0 mg/l) induced shoot initiation after 7 days of post-inoculation. The nodal segments were then excised and treated with various concentrations of BAP, BAP with KIN, and coconut water for the proliferation among which 5 mg/l induced significant nodal segment proliferation (9.0 nodal segments/per explant) and shoot length (8.0 cm). The efficacy of coconut water in increasing the nodal segment proliferation of T. cordifolia was tested and the shoot proliferation increased significantly at 5% and 10% of coconut water, however, the maximum response of shoot number (23.0), shoot length (12 cm) was in the MS medium supplementation with BAP (5mg/l) and 10% coconut water. The genetic fidelity of these plants was also confirmed by random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers in wild and in vitro cultures. This protocol is an efficient way for the in vitro mass propagation of true-to-type plantlets of T. cordifolia which provides a basis for germplasm conservation and sustainable utilization.

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“…There were 498 detections (21.67%) of a repeating pattern of AG/CT among these SSRs. As a result of this research, Lade et al (2020) have gained deeper insight into the molecular mechanisms that regulate the growth, development and metabolism of G. elata (Tianma). Total 96 sample of Tinospora cordifolia were gathered from 10 different geographically diverse locales of the India.…”

Section: Development Of Plant Molecular Markersmentioning

confidence: 99%

Upcoming progress of transcriptomics studies on plants: An overview

Tyagi

Singh²,

Mathur³

et al. 2022

Front. Plant Sci.

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Transcriptome sequencing or RNA-Sequencing is a high-resolution, sensitive and high-throughput next-generation sequencing (NGS) approach used to study non-model plants and other organisms. In other words, it is an assembly of RNA transcripts from individual or whole samples of functional and developmental stages. RNA-Seq is a significant technique for identifying gene predictions and mining functional analysis that improves gene ontology understanding mechanisms of biological processes, molecular functions, and cellular components, but there is limited information available on this topic. Transcriptomics research on different types of plants can assist researchers to understand functional genes in better ways and regulatory processes to improve breeding selection and cultivation practices. In recent years, several advancements in RNA-Seq technology have been made for the characterization of the transcriptomes of distinct cell types in biological tissues in an efficient manner. RNA-Seq technologies are briefly introduced and examined in terms of their scientific applications. In a nutshell, it introduces all transcriptome sequencing and analysis techniques, as well as their applications in plant biology research. This review will focus on numerous existing and forthcoming strategies for improving transcriptome sequencing technologies for functional gene mining in various plants using RNA- Seq technology, based on the principles, development, and applications.

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Estimation of genetic diversity and population structure in Tinospora cordifolia using SSR markers

Cited by 9 publications

References 40 publications

Assessment of genetic diversity and population structure of the genus Vicia (Vicia L.) using simple sequence repeat markers

Assessment of genetic diversity and population structure of the genus Vicia (Vicia L.) using simple sequence repeat markers

In vitro Propagation and Assessment of Genetic Homogeneity using RAPD and ISSR Markers in Tinospora cordifolia (Wild.) Hook. F. & Thoms, An Important Medicinal Plant of Nepal

Upcoming progress of transcriptomics studies on plants: An overview

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