Estimation of heritability of atopic dermatitis in Labrador and Golden Retrievers

Shaw, Stephen; Wood, James L. N.; Freeman, Julia; Littlewood, Janet D.; Hannant, D.

doi:10.2460/ajvr.2004.65.1014

Cited by 48 publications

(37 citation statements)

References 14 publications

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“…The signals elevated in intensity in the Golden Retriever plasma appeared to be sphingolipids, sphingomyelins and phosphatidylcholines. This observation may be consistent with previous evidence of the role of sphingolipids in skin disorders in Golden Retrievers, which are prone to atopic dermatitis (Shaw et al 2004). Healthy dogs of mixed breeds have been shown to have erythrocytes that contain increased concentrations both of saturated sphingomyelin and also some phosphatidylcholines when compared to dogs suffering with atopic dermatitis (Fuhrmann et al 2006).…”

Section: Resultssupporting

confidence: 93%

Ultra high performance liquid chromatography–high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets

et al. 2017

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Introduction and objectivesThe purpose of this study was to use high accurate mass metabolomic profiling to investigate differences within a phenotypically diverse canine population, with breed-related morphological, physiological and behavioural differences. Previously, using a broad metabolite fingerprinting approach, lipids appear to dominate inter- and intra- breed discrimination. The purpose here was to use Ultra High Performance Liquid Chromatography–High Resolution Mass Spectrometry (UHPLC–HRMS) to identify in more detail, inter-breed signatures in plasma lipidomic profiles of home-based, client-owned dogs maintained on different diets and fed according to their owners’ feeding regimens.MethodsNine dog breeds were recruited in this study (Beagle, Chihuahua, Cocker Spaniel, Dachshund, Golden Retriever, Greyhound, German Shepherd, Labrador Retriever and Maltese: 7–12 dogs per breed). Metabolite profiling on a MTBE lipid extract of fasted plasma was performed using UHPLC-HRMS.ResultsMultivariate modelling and classification indicated that the main source of lipidome variance was between the three breeds Chihuahua, Dachshund and Greyhound and the other six breeds, however some intra-breed variance was evident in Labrador Retrievers. Metabolites associated with dietary intake impacted on breed-associated variance and following filtering of these signals out of the data-set unique inter-breed lipidome differences for Chihuahua, Golden Retriever and Greyhound were identified.ConclusionBy using a phenotypically diverse home-based canine population, we were able to show that high accurate mass lipidomics can enable identification of metabolites in the first pass plasma profile, capturing distinct metabolomic variability associated with genetic differences, despite environmental and dietary variability.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-016-1152-0) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 93%

Ultra high performance liquid chromatography–high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets

et al. 2017

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“…Canine atopic dermatitis (AD) is a common inflammatory allergic skin disorder and results from a combination of genetic and environmental factors [1]. Changes in the structure, function and composition of the epidermis are present in atopic dogs compared with normal dogs, and may predispose to the development of the disease by enhancing percutaneous absorption and antigen presentation to the immune system [2].…”

Section: Resultsmentioning

confidence: 99%

Real-time PCR quantification of the canine filaggrin orthologue in the skin of atopic and non-atopic dogs: a pilot study

et al. 2011

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BackgroundCanine atopic dermatitis (AD) is a common inflammatory skin disease associated with defects in the epidermal barrier, particularly in West Highland white terriers (WHWTs). It shares many similarities with human AD, and so may be a useful animal model for this disease. Epidermal dysfunction in human AD can be caused by mutations in the gene encoding the epidermal protein filaggrin (FLG) and, in some atopic patients, be associated with altered FLG mRNA and protein expression in lesional and/or non-lesional skin. In experimental models of canine AD, mRNA expression of the orthologous canine filaggrin gene may be reduced in non-lesional skin compared with healthy controls. However, there is no published data on canine filaggrin mRNA expression in the skin of dogs with naturally-occurring AD. Hence, the aim of this pilot study was to develop a reverse transcriptase real-time PCR assay to compare filaggrin mRNA expression in the skin of atopic (n = 7) and non-atopic dogs (n = 5) from five breeds, including eight WHWTs.FindingsOverall, filaggrin mRNA expression in non-lesional atopic skin was decreased compared to non-lesional non-atopic skin (two fold change); however this difference was only statistically significant in the subgroup of WHWTs (P = 0.03).ConclusionsAlthough limited by the small sample size, these results indicate that, comparable to some cases of human AD, altered filaggrin mRNA expression may exist in the skin of some atopic dogs with naturally-occurring disease. Additional studies, including larger sample numbers, will be necessary to confirm this finding and to investigate whether mutations in the filaggrin gene exist and contribute to epidermal lesions of AD in dogs.

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“…In a subsequent study of geographical risk factors, increased incidence was seen with increasing annual rainfall, increasing human population density and among dogs living in the same county as a veterinary dermatologist 9 . CAD has a strong genetic component, and in a study of golden and Labrador retrievers the heritability of the disease was estimated as 0.47 10 . It thus seems reasonable to suppose that environmental factors in combination with genetic make‐up are important for the development of the disease, as described for the human counterpart.…”

Section: Introductionmentioning

confidence: 99%

A case–control study of risk factors for canine atopic dermatitis among boxer, bullterrier and West Highland white terrier dogs in Sweden

Nødtvedt

Bergvall

Sallander

et al. 2007

Veterinary Dermatology

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Environmental and dietary risk factors for the development of canine atopic dermatitis (CAD) in the high-risk breeds of boxer, bullterrier and West Highland white terrier were assessed in a case-control study. A logistic regression model was developed to evaluate their relative importance in 58 cases from 12 practices in Sweden and 61 unaffected controls, matched to cases by breed and year of birth. The final model included a random error term for 'examining veterinarian', as dogs from the same practice were not considered independent. No effect of gender, season of birth, environment, vaccination or de-worming practices on the odds of developing CAD was detected. The main finding was that feeding a diet including noncommercial products to the bitch during lactation had a protective effect on the development of CAD in her offspring; the odds of developing CAD were twice as high among offspring from bitches that were not exposed to home-made/noncommercial diets [95% confidence interval (CI) of the odds ratio: 1.2-3.8]. The population attributable fraction for not feeding home-made diets to the lactating bitch was estimated as 0.4 (95% CI: 0.04-0.63). Randomized controlled clinical trials are needed to further support the finding of a potential protective role of diet in CAD development.

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Estimation of heritability of atopic dermatitis in Labrador and Golden Retrievers

Abstract: Atopic dermatitis has a strong genetic component, and breeding of dogs with clinical signs of atopic dermatitis should be discouraged.

Cited by 48 publications

References 14 publications

Ultra high performance liquid chromatography–high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets

Ultra high performance liquid chromatography–high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets

Real-time PCR quantification of the canine filaggrin orthologue in the skin of atopic and non-atopic dogs: a pilot study

A case–control study of risk factors for canine atopic dermatitis among boxer, bullterrier and West Highland white terrier dogs in Sweden

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