2009
DOI: 10.1371/journal.pone.0007847
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Estimation of Immune Cell Densities in Immune Cell Conglomerates: An Approach for High-Throughput Quantification

Abstract: BackgroundDetermining the correct number of positive immune cells in immunohistological sections of colorectal cancer and other tumor entities is emerging as an important clinical predictor and therapy selector for an individual patient. This task is usually obstructed by cell conglomerates of various sizes. We here show that at least in colorectal cancer the inclusion of immune cell conglomerates is indispensable for estimating reliable patient cell counts. Integrating virtual microscopy and image processing … Show more

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Cited by 32 publications
(32 citation statements)
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“…Although the thickness of the sections is less than half the average diameter of a lymphocyte, the reported CD4 + cell number may to some extent differ from its true count. 25 Our calculation of CD4 + T lymphocytes may be slightly underestimated because antibodies to CD8 also recognize some γδ T cells and natural killer cells (Dako Denmark).…”
Section: Discussionmentioning
confidence: 99%
“…Although the thickness of the sections is less than half the average diameter of a lymphocyte, the reported CD4 + cell number may to some extent differ from its true count. 25 Our calculation of CD4 + T lymphocytes may be slightly underestimated because antibodies to CD8 also recognize some γδ T cells and natural killer cells (Dako Denmark).…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, we have developed a novel system to automate the process of whole slide tissue preparation and staining and subsequent objective evaluation (10)(11)(12). Not only centers of the primary CRC tumor but also adjacent normal mucosal tissues were evaluated ( Supplementary Fig.…”
Section: Evaluation Of Immunostaining In Tissue Samplesmentioning
confidence: 99%
“…S2). High-precision quantification of cell densities was performed as described previously (11,12) Chemokine and cytokine detection in tissue lysates and corresponding sera Lysates were prepared according to the manufacturer's instructions (BioRad Laboratories). A 2-laser reader simultaneously quantifies cytokines and chemokines.…”
Section: Evaluation Of Immunostaining In Tissue Samplesmentioning
confidence: 99%
“…Besides common computer vision tasks like object detection, segmentation and recognition, histopathological imaging poses domain specific problems such as estimating staining of nuclei conglomerates [39] and differentiation nuclei by their shape [40].…”
Section: Imaging: From Classical Image Processing To Statistical Pattmentioning
confidence: 99%