Estimation of inactivation effects against <i>Escherichia coli</i> O157:H7 biofilm by different plasma-treated solutions and post-treatment storage

An, Jeong Yeon; Yong, Hae In; Kim, Hyun‐Joo; Park, Joo Young; Lee, Sang Hui; Baek, Ki Ho; Choe, Wonho; Jo, Cheorun

doi:10.1063/1.5082657

Cited by 21 publications

(18 citation statements)

References 33 publications

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“…(6 – 7) ( Jo et al, 2020 ). Peroxynitrite is a strong oxidant and bactericidal compound that causes oxidative stress, denaturation of the cell wall, and DNA damage ( An et al, 2019 ). For these reasons, nitrite addition improves the microbial safety of meat products.…”

Section: The Role Of Nitrite In Meat Productsmentioning

confidence: 99%

Clean Label Meat Technology: Pre-Converted Nitrite as a Natural Curing

Yong

Kim

Choi

et al. 2021

Food Sci Anim Resour

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Clean labeling is emerging as an important issue in the food industry, particularly for meat products that contain many food additives. Among synthetic additives, nitrite is the most important additive in the meat processing industry and is related to the development of cured color and flavor, inhibition of oxidation, and control of microbial growth in processed meat products. As an alternative to synthetic nitrite, pre-converted nitrite from natural microorganisms has been investigated, and the applications of pre-converted nitrite have been reported. Natural nitrate sources mainly include fruits and vegetables with high nitrate content. Celery juice or powder form have been used widely in various studies. Many types of commercial starter cultures have been developed. S. carnosus is used as a critical nitrate reducing microorganism and lactic acid bacteria or other Staphylococcus species also were used. Pre-converted nitrite has also been compared with synthetic nitrite and studies have been aimed at improving utilization by exploiting the strengths (positive consumer attitude and decreased residual nitrite content) and limiting the weaknesses (remained carcinogenic risk) of pre-converted nitrite. Moreover, as concerns regarding the use of synthetic nitrites increased, research was conducted to meet consumer demands for the use of natural nitrite from raw materials. In this report, we review and discuss various studies in which synthetic nitrite was replaced with natural materials and evaluate pre-converted nitrite technology as a natural curing approach from a clean label perspective in the manufacturing of processed meat products.

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Section: The Role Of Nitrite In Meat Productsmentioning

confidence: 99%

Clean Label Meat Technology: Pre-Converted Nitrite as a Natural Curing

Yong

Kim

Choi

et al. 2021

Food Sci Anim Resour

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“…a self-produced biopolymeric matrix that affords microorganism protection, remain elusive. 15,16 Furthermore, these in vitro findings may not necessarily translate to real-life applications where PAW is expected to kill pathogens within a complex, multiorganism system. 17 Indeed, prolonged activation of water can lead to significant localized changes in its pH and concentration gradients of reactive species.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Indeed, although many studies have shown that PAW possesses unique biochemical reactivity to inhibit a wide range of planktonic microorganisms and even spores, ,, and do so with high selectivity, , most of these studies were conducted in vitro using simple systems, e.g., a suspension of a single species of pathogenic microorganisms. , Relevant mechanisms that underlie the biochemical activity of the agents generated by plasmas in a solution against pathogens within the biofilm, i.e. a self-produced biopolymeric matrix that affords microorganism protection, remain elusive. , Furthermore, these in vitro findings may not necessarily translate to real-life applications where PAW is expected to kill pathogens within a complex, multiorganism system . Indeed, prolonged activation of water can lead to significant localized changes in its pH and concentration gradients of reactive species.…”

Section: Introductionmentioning

confidence: 99%

Microplasma Bubbles: Reactive Vehicles for Biofilm Dispersal

Zhou¹,

Zhou²,

Wang³

et al. 2019

ACS Appl. Mater. Interfaces

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Interactions between effects generated by cold atmospheric-pressure plasmas and water have been widely investigated for water purification, chemical and nanomaterial synthesis, and, more recently, medicine and biotechnology. Reactive oxygen and nitrogen species (RONS) play critical roles in transferring the reactivity from gas plasmas to solutions to induce specific biochemical responses in living targets, e.g., pathogen inactivation and biofilm removal. While this approach works well in a single-organism system at a laboratory scale, integration of plasma-enabled biofilm removal into complex real-life systems, e.g., large aquaculture tanks, is far from trivial. This is because it is difficult to deliver sufficient concentrations of the right kind of species to biofilm-covered surfaces while carefully maintaining a suitable physiochemical environment that is healthy for its inhabitants, e.g., fish. In this work, we show that underwater microplasma bubbles (generated by a microplasma-bubble reactor that forms a dielectric barrier discharge at the gas–liquid interface with the applied voltage of 4.0 kV) act as transport vehicles to efficiently deliver reactive plasma species to the target biofilm sites on artificial and living surfaces while keeping healthy water conditions in a multispecies system. The as-generated air microplasma bubbles and plasma-activated water (PAW) both can effectively reduce the existing pathogenic biofilm load by ∼83 and 60%, respectively, after 15 min of discharge at 40 W and prevent any new biofilm from forming. The generation of underwater microplasma bubbles in a custom-made fish tank for less than a minute per day (20 s per time, twice daily) can introduce sufficient quantities of RONS into PAW to reduce the biofilm-infected area by ∼80–90% and improve the health status of Cichlasoma synspilum × Cichlasoma citrinellum blood parrot cichlid fish. Species generated include hydrogen peroxide, ozone, nitrite, nitrate, and nitric oxide. Using mimicked chemical solutions, we show that the plasma-induced nitric oxide acts as a critical bioactive species that triggers the release of cells from the biofilm and their inactivation.

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“…Indeed, several previous studies have shown that PAW has a notable broad-spectrum biocidal activity against bacteria, ,, fungus, and biofilms . The ROS and RNS in PAW, or their synergy, play a crucial role in its biocidal activity, which results in a high oxidation–reduction potential and low pH value.…”

Section: Resultsmentioning

confidence: 99%

Potassium Iodide Potentiates Bacterial Killing by Helium Atmospheric Pressure Plasma Jet

Zhang

Huang

2019

ACS Omega

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Cold atmospheric plasma (CAP) is an effective new antimicrobial approach that is gaining increasing attention and has a wide range of potential applications in biomedical fields. Among all of the bactericidal factors generated by CAP, the synergy of reactive nitrogen species (RNS) and reactive oxygen species is generally considered as the main reason for its high bactericidal efficiency. However, the produced RNS (such as nitrite) may also pose potential risks to human health. Therefore, it is of significance to keep the high disinfection efficiency of CAP but with producing no or little harmful RNS. In this study, we investigated whether it is possible to improve the disinfection efficiency of CAP without producing the harmful RNS by adding a certain amount of inert halogen salt such as potassium iodide (KI). We found that the inactivation of both Gram-negative and Gram-positive bacteria by helium atmospheric pressure plasma jet (He-APPJ), one form of CAP, is enhanced consistently in the presence of a certain amount of KI. The mechanism of action is due to the fact that the He-APPJ-generated hydrogen peroxide (H 2 O 2 ) oxidizes the iodide anion to triiodide (I 3 – ), which contributes to the major bactericidal activity. We believe that the results in this work can be highly relevant to the practical application of plasma for disinfection in the biomedical field.

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Estimation of inactivation effects against Escherichia coli O157:H7 biofilm by different plasma-treated solutions and post-treatment storage

Cited by 21 publications

References 33 publications

Clean Label Meat Technology: Pre-Converted Nitrite as a Natural Curing

Clean Label Meat Technology: Pre-Converted Nitrite as a Natural Curing

Microplasma Bubbles: Reactive Vehicles for Biofilm Dispersal

Potassium Iodide Potentiates Bacterial Killing by Helium Atmospheric Pressure Plasma Jet

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