Mechanomyography (MMG) is an important muscle physiological activity signal that can reflect the amount of motor units recruited as well as the contraction frequency. As a result, MMG can be utilized to estimate the force produced by skeletal muscle. However, cross-talk and time-series correlation severely affect MMG signal recognition in the real world. These restrict the accuracy of dynamic muscle force estimation and their interaction ability in wearable devices. To address these issues, a hypothesis that the accuracy of knee dynamic extension force estimation can be improved by using MMG signals from a single muscle with less cross-talk is first proposed. The hypothesis is then confirmed using the estimation results from different muscle signal feature combinations. Finally, a novel model (improved grey wolf optimizer optimized long short-term memory networks, i.e., IGWO-LSTM) is proposed for further improving the performance of knee dynamic extension force estimation. The experimental results demonstrate that MMG signals from a single muscle with less cross-talk have a superior ability to estimate dynamic knee extension force. In addition, the proposed IGWO-LSTM provides the best performance metrics in comparison to other state-of-the-art models. Our research is expected to not only improve the understanding of the mechanisms of quadriceps contraction but also enhance the flexibility and interaction capabilities of future rehabilitation and assistive devices.