Estimation of light intensity distribution in a culture vessel

Ibaraki, Yasuomi; Nozaki, Yuko

doi:10.1007/s11240-004-5936-y

Cited by 7 publications

(3 citation statements)

References 5 publications

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“…Os altos custos na produção de mudas por cultura de tecidos, entre outros fatores, relacionam-se ao alto consumo de energia elétrica nos laboratórios, principalmente em salas de crescimento (ERIG & SCHUCH, 2005;GRATTAPAGLIA & MACHADO, 1998;KODYM & ZAPATA-ARIAS, 1999;SAVANGIKAR, 2004;BRAGA, et al, 2009), e à baixa eficiência de aclimatização por causa da heterotrofia dos tecidos cultivados, resultantes do ambiente imposto pelas condições de cultivo in vitro (ARIGITA et al, 2002;KOZAI & KUBOTA, 2001). Entre os fatores relacionados à condição heterotrófica das plantas in vitro, destaca-se (ECONOMOU & READ, 1987;IBARAKI & NOZAKI, 2005) a adição de fonte externa de luz e carboidrato ao meio de cultura (KOZAI et al, 1991;PRAKASH et al, 2004;REZENDE et al, 2008;SANTANA et al, 2008;SERRET et al, 1997). Essa condição de cultivo, resulta em diversas alterações morfológicas e fisiológicas de plântulas cultivadas in vitro, que são responsáveis por grande parte das perdas durante a aclimatização.…”

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Luz natural e concentrações de sacarose no cultivo in vitro de Cattleya walkeriana

et al. 2009

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RESUMOCattleya walkeriana é uma das mais apreciadas orquídeas do mundo, possuindo grande valor ornamental. A micropropagação é uma importante ferramenta para reprodução dessa espécie. Pelos altos custos de produção através da micropropagação convencional, relacionados à perdas durante aclimatização e alto consumo de energia em salas de crescimento, objetivou-se com este trabalho avaliar estratégias alternativas no cultivo in vitro. Foram testados dois diferentes fatores, sendo eles luz (em sala de crescimento convencional, casa de vegetação com sombreamento de 50%, e casa de vegetação sem sombreamento) e concentrações de sacarose (0, 15 e 30 g.L -1 ). O material se constitui de plântulas previamente germinadas in vitro, em meio MS e cultivadas em sala de crescimento por 90 dias. Luz natural em casa de vegetação, resultou em aumento no número de brotos, na espessura foliar e na freqüência e diâmetro dos estômatos, e ocasionou diminuição do comprimento das plântulas e dos teores de clorofila. Um menor número de raízes e brotações foram formados na concentração de 15 g.L -1 de sacarose. A ausência de sacarose resultou em menores teores de clorofila. Pela análise dos resultados, foi possível concluir que o ambiente de cultivo altera as respostas de plântulas cultivadas in vitro e pode-se recomendar o uso da luz natural e redução nas concentrações de sacarose pela metade para a micropropagação de Cattleya walkeriana, observandose a fase da propagação e os objetivos da mesma. Termos para indexação: Orquídea, micropropagação, anatomia ABSTRACTCattleya walkeriana is one of the most important orchids in the world and has high ornamental value. Micropropagation plays an important role in the reproduction of this species. Based on high production costs of the standard micropropagation regarding the losses during acclimatization and high energy consumption in growth chambers, this paper aimed at evaluating alternative strategies for the in vitro cultivation. Two different factors were tested: light (standard growth chamber, greenhouse with 50% shading, and greenhouse without shading) and sucrose concentrations (0, 15, and 30g.L -1 ). The material was composed by plantlets previously germinated in vitro on MS medium and cultivated in a growth chamber for 90 days. Natural light in greenhouse induced an increase in the number of shoots, leaf thickness, stomata frequency and diameter and, on the other hand, caused decrease in the plantlet length and chlorophyll levels. Lower number of roots and shoots were formed at the concentration of 15g.L -1 sucrose. The absence of sucrose resulted in lower chlorophyll levels. Based on these results, it was possible to conclude that the cultivation environment modifies the response of in vitro cultivated plantlets and the natural light and reduction of sucrose concentrations by half can be recommend for the micropropagation of Cattleya walkeriana, taking in consideration the propagation phases and their goals.

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Luz natural e concentrações de sacarose no cultivo in vitro de Cattleya walkeriana

et al. 2009

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“…The temperature and light intensity in the greenhouse were measured using type-t thermocouples and a photosynthetic photon flux (PPF) sensor (IKS-27; Koito Industries, Japan), respectively, connected to a hybrid recorder (HR1300; Yokogawa, Japan). A solar irradiance measurement film system (OPTLEAF Y-1W; Taisei Chemical Industries, Japan) was used to estimate PPF received by leaves (Ibaraki and Nozaki, 2005); five films were placed on individual bent shoot layers, and were sampled and replaced new films at intervals of 15 days.…”

Section: Cultivation Of Rose Plantsmentioning

confidence: 99%

Contribution of Individual Bent Shoot Layers to Cut Flower Productivity in Shoot-bending ‘Asami·Red’ Roses

Doi

Shimomura

Inamoto

et al. 2009

J. Japan. Soc. Hort. Sci.

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We estimated the contribution of individual shoot layers in a multi-layered bent canopy to the cut flower productivity of roses ('Asami·Red'). A two-layered bent shoot canopy was established and upper-or lower-layer bent shoots were replaced with artificial replicas of shoots. Replacing upper-layer shoots with replicas facilitated leaf abscission of lower-layer shoots, resulting in severe reduction in the total dry weight of cut flowers (TDWF) compared with plants having two intact bent shoots. When lower-layer shoots were replaced with replicas, the leaf area of upper-layer shoots increased and consequently the excision of lower-layer shoots had little influence on the TDWF. In another experiment, when a three-layered bent shoot canopy was established and light interception by lower-layer shoots was restricted completely by placing a shading film between two of the three layers, the leaf area of the uppermost shoots increased to compensate for the decreased leaf area due to leaf abscission of lower-layer shoots. The excision of lower-layer shoots resulted in more severe reduction of the TDWF than shading. These results suggest that in a multi-layered bent shoot canopy of roses, upper-layer shoots contribute to cut flower yield of plants, primarily through their current photosynthesis with high light interception. However, lower-layer shoots also contribute to cut flower yield by exporting assimilates retained previously, and hence these shoots should remain on plants until they die spontaneously.

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“…However, since Ф PSII depends on the level of light irradiating a leaf, careful consideration is required for determining photosynthetic properties solely from values of Ф PSII . In particular, in micropropagation, it is difficult to exactly determine the light intensity on a plantlet leaf inside a culture vessel because the light intensity inside the vessel actually differs from that outside (Ibaraki and Nozaki 2005). Therefore, the use of Fv/Fm is desirable for routine estimation of micropropagated plant leaves within the culture vessel.…”

Section: Introductionmentioning

confidence: 99%

Nondestructive evaluation of the photosynthetic properties of micropropagated plantlets by imaging photochemical reflectance index under low light intensity

Ibaraki

Gupta

2010

In Vitro Cell.Dev.Biol.-Plant

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The photochemical reflectance index (PRI) of micropropagated potato leaves was estimated nondestructively from outside the culture vessel using a PRI imaging system developed by the present group. The PRI was determined under low light intensity conditions after dark treatment and compared with the chlorophyll fluorescence parameter Fv/Fm, which denotes photosystem II maximum quantum yield. Short-term high-light treatment decreased Fv/Fm of the plantlets. Culture conditions such as temperature and sucrose concentration also affected Fv/Fm. A linear relationship between the PRI and Fv/Fm was observed in both cases of high-light treatment and different culture conditions, suggesting the potential of the PRI to be used as a substitute for Fv/Fm. PRI estimated from reflection images under low light intensity conditions may be used for rapid and noninvasive evaluation of photosynthetic properties of micropropagated plantlets in a similar manner to Fv/Fm.

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Estimation of light intensity distribution in a culture vessel

Cited by 7 publications

References 5 publications

Luz natural e concentrações de sacarose no cultivo in vitro de Cattleya walkeriana

Luz natural e concentrações de sacarose no cultivo in vitro de Cattleya walkeriana

Contribution of Individual Bent Shoot Layers to Cut Flower Productivity in Shoot-bending ‘Asami·Red’ Roses

Nondestructive evaluation of the photosynthetic properties of micropropagated plantlets by imaging photochemical reflectance index under low light intensity

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