Estimation of Meloxicam in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry (Lc-MS/Ms)

Ganesh, Mani; Hemalatha, Pushparaj; Peng, Mei Mei; Sakthimanigandan, Kalaimani; Jang, Hee-Jin

doi:10.1080/10826076.2012.678453

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…In a previous study, liquid-liquid extraction was used to prepare samples. However, interference peaks were found after liquid-liquid extraction and the extraction recovery rate was 60.8% (Ganesh et al, 2013). The protein precipitation method developed by us achieves a recovery rate ranging between 91.6% and 94.8%.…”

Section: Optimization Of the Preparation Procedures And Lc-ms/ms Conditionmentioning

confidence: 98%

“…A number of analytical methods for meloxicam determination in biological samples were reported, including LC-MS/MS (Ganesh et al, 2013;Lee et al, 2009;Pairis-Garcia et al, 2015;Shirako et al, 2013;Tian et al, 2018;Wiesner et al, 2003;Yuan et al, 2007), reverse-phase-HPLC (Cox et al, 2017;Helmy & Bedaiwy, 2013;Lehr et al, 2010;Sahoo et al, 2014;Sim & Cox, 2018) and HPLC-UV (Bae et al, 2011;Ghani, 2020;Miyamoto et al, 2017;Tekeli et al, 2020;Zeeb et al, 2018). In recent years, MS has become a general detection method for in vivo drug analysis.…”

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confidence: 99%

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Using a stable isotope‐labeled internal standard for liquid chromatography–tandem mass spectrometry quantitation of meloxicam in human plasma

Zhan

Wang

et al. 2021

Biomedical Chromatography

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A sensitive and highly efficient LC-ESI-MS/MS method using a stable isotopelabeled internal standard (SIL IS) to detect meloxicam in human plasma was developed and validated. Sample preparation used only 50 μL human plasma with one-step methanol protein precipitation. A gradient mobile phase system was adopted for chromatographic separation on a Poroshell 120 SB-C 18 column (2.1 Â 50 mm, 2.7 μm). Positive ion pattern was chosen for quantification under multiple reaction monitoring. Ion pairs were [M + H] + m/z 352.1 ! 115.1 for meloxicam and [M + H] + m/z 355.1 ! 187.1 for meloxicam-d3 (SIL IS). Total run time was 4.0 min. Standard curve was linear over a concentration range from 8.00 to 1600 ng mL À1 .This method was fully validated to evaluate its performance, including specificity, carryover, sensitivity, linearity, accuracy, precision, recovery, matrix effects, stability, dilution reliability and incurred sample reanalysis, which provided a reliable basis for pharmacokinetic studies of meloxicam in 28 healthy Chinese volunteers. After a single-dose oral administration of 7.5 mg meloxicam, the main pharmacokinetic parameters were as follows:

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Section: Optimization Of the Preparation Procedures And Lc-ms/ms Conditionmentioning

confidence: 98%

mentioning

confidence: 99%

Using a stable isotope‐labeled internal standard for liquid chromatography–tandem mass spectrometry quantitation of meloxicam in human plasma

Zhan

Wang

et al. 2021

Biomedical Chromatography

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Determination of piroxicam from rat articular tissue and plasma based on LC–MS/MS

Kim

Cho

et al. 2016

Arch. Pharm. Res.

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Osteoarthritis (OA) is the most common type of arthritis. To manage OA, in general, oral administration of non-steroidal anti-inflammatory drugs (NSAIDs) is used. Recently, the analgesic and anti-inflammatory efficacy of piroxicam (PX), a long-acting NSAID, by intra-articular (IA) administration in OA was reported, and the possibility that PX is distributed in articular tissues at a certain concentration was raised. Thus, herein, novel LC-MS/MS methods to detect PX in rat articular tissue and plasma are presented. For articular tissue, solvent extraction with acetonitrile for 12 h was employed and a protein precipitation method was used for the preparation of a plasma sample. The developed methods were validated by following the FDA guidelines, and the validated methods were successfully applied to a PK study of IA PX. The present study presents, to our knowledge, the first method of determining a drug in articular tissue. Additionally, the level of PX in articular tissue after IA PX administration was experimentally confirmed for the first time using the present methods. Therefore, the present methods provide a new direction for in vivo evaluation for IA PX formulations and contribute to the development of alternative IA PX formulations with better effects for the treatment of OA.

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Meloxicam

Khalil

Aldosari

2020

Profiles of Drug Substances, Excipients and Related Methodology

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Estimation of Meloxicam in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry (Lc-MS/Ms)

Cited by 5 publications

References 16 publications

Using a stable isotope‐labeled internal standard for liquid chromatography–tandem mass spectrometry quantitation of meloxicam in human plasma

Using a stable isotope‐labeled internal standard for liquid chromatography–tandem mass spectrometry quantitation of meloxicam in human plasma

Determination of piroxicam from rat articular tissue and plasma based on LC–MS/MS

Meloxicam

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