Estimation of mutagenic/carcinogenic potential of environmental contaminants by ion-molecule reactions and tandem mass spectrometry

Abstract

The ability to produce and detect products of model DNA/carcinogen ion-molecule reactions is demonstrated in the ion source and the collision cell of a triple quadrupole tandem mass spectrometer. Reaction between adenine and benzoyl chloride in the ion source is shown to produce the DNA adduct benzoyl adenine. The daughter ion mass spectrum of the reaction product is compared to that of the synthesized standard. Mass chromatograms of the reaction between mass-selected pyridine ions and various analytes eluting from a GC column into the collision cell are demonstrated and illustrate the ability to detect only the GC eluates that react with pyridine. This technique could provide a rapid and sensitive method for screening complex environmental samples for carcinogens, as well as for estimating the relative mutagenic/carcinogenic potential of environmental contaminants.

Perspectives on the FUTURE OF ANALYTICAL MASS SPECTROMETRY

“…We have also studied the gas-phase reactivity of known carcinogens with various nucleophiles using MS/MS. These studies clearly demonstrate the power of "chemistry" inside (or on line with) a mass spectrometer (15).…”