2023
DOI: 10.31344/ijhhs.v7i2.547
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Estimation of Nicotine in Tobacco Extract Using C8 Column in High Performance Liquid Chromatography

Abstract: Background: Nicotine is the most used toxic substance in spite of mass media awareness. Column used in the estimation of nicotine is found to be the inhibiting factor for its estimation.Objective: To develop a method for nicotine estimation on C8 column in HPLC.Methods: The method involved a model Waters 1515 binary HPLC Pump system interfaced with a 2489 Waters UV/VIS detector, a 4.6 X 250 mm, 5μm beads Symmetry C8 column at 37°C, and an isocratic mobile phase containing 60%: 40% v/ v mixture of 10mM sodium a… Show more

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