Estimation of phylogenetic relationships of Phlomis species based on seed protein polymorphism

Yüzbaşıoğlu, Ertuğrul; Dadandi, Mehmet Yaşar; Özcan, Servet

doi:10.2225/vol12-issue2-fulltext-7

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…Thus, recent phylogenetic studies based on seed proteins have been carried out in wheat (Alvarez et al. , 2006), Cucumis (Singh & Matta, 2008) and Phlomis species (Yüzbaşıoğlu, 2009).…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of Orobanche and Phelipanche species inferred from seed proteins

Castillejo¹,

Fernández‐Aparicio²,

Šatović³

et al. 2009

Weed Research

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Summary Orobanche and Phelipanche species are root parasitic plants some of which infect important crops, causing extensive damage. In an attempt to unravel molecular systematic in these genera using protein markers, we established the protein profile of twelve species by two‐dimensional gel electrophoresis (2‐DE). Proteins were detected by Sypro Ruby staining and the gel images were digitalised and analysed. The 2‐DE maps contained from 30 to 140 protein spots for the analysed species. A virtual master gel was built with spots present in all species and revealing the presence of 260 protein spots. Only one protein was monomorphic, while 143 were unique to a single species. Proteins were scored for the presence or absence of homologous spots to create a binary matrix. Phylogenetic analysis was carried out using maximum parsimony and distance method. The trees obtained were rooted using Cistanche phelypaea as an out‐group. Both analyses showed Orobanche as a monophyletic group. AMOVA analysis revealed phenotypic diversity between species within genera, as well as a considerable level of variation between genera. This is a first step to help in the phylogenetic classification of parasitic plants by means of proteomics.

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“…Thus, recent phylogenetic studies based on seed proteins have been carried out in wheat (Alvarez et al. , 2006), Cucumis (Singh & Matta, 2008) and Phlomis species (Yüzbaşıoğlu, 2009).…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of Orobanche and Phelipanche species inferred from seed proteins

Castillejo¹,

Fernández‐Aparicio²,

Šatović³

et al. 2009

Weed Research

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“…From each of the 27 studied species, five representative grains were bulked (Yüzbaşıoglu et al 2009) and crushed using a pestle and mortar and the gliadins were extracted by adding 70% ethanol in a 3.3 volume of the grain weight, vortexed and mixed for 2 h then centrifuged for 15 min at 14,000 rpm in an Eppendorf microcentrifuge. A volume of 100 ll from the supernatant was added to 85 ll of 60% (v/v) glycerin, and 40 ll of the mixture was run on 6% acrylamide gels (160 9 80 9 1.5 mm, Hoefer SE-600) using an electric current of 50 mA for 3.30 h. Each gel contained 13 samples in addition to the Canadian variety Marquis as a control.…”

Section: A-pagementioning

confidence: 99%

“…The utility of seed storage proteins (A-PAGE and SDS-PAGE) and PCR-based randomly amplified polymorphic DNAs (RAPDs) or inter-simple sequence repeats (ISSRs) variations as phylogenetic markers for investigating evolutionary relationships among plant species has been clearly established (Yüzbaşıoglu et al 2009;Archibald et al 2006;Yorgancilar et al 2009;Verma et al 2009). Thus, in the present study, the efficiency of these methods in revealing genetic relationships among plant species using the well-studied Triticum-Aegilops group as a model was investigated and compared since genetic relationships among those species have been well documented.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

Haider

Nabulsi

MirAli

2010

Genet Resour Crop Evol

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Species that belong to the genus Triticum L. and the genetically related Aegilops L. genus are important genetic and economic resources because they have an evolutionary relationship with the two main agricultural crops T. aestivum (bread wheat) and T. durum (durum wheat). Therefore, it is important to understand the genetic relationships among the cultivated wheat species and their wild relatives. The latter have a great role in the improvement of cultivated wheat. Molecular markers are the best choice and most reliable means to study these relationships accurately. In this study, we compared the efficiency of the biochemical methods A-PAGE and SDS-PAGE on seed storage proteins and the molecular methods RAPDs and ISSRs to explore the genetic relationships among seven species of Triticum and 20 Aegilops species. Three phylogenetic trees obtained in this study were compared with available classifications and phylogenetic trees constructed earlier for these species. It was noted that the tree based on ISSRs data was the most congruent with those classification and trees. This may be attributed to the fact that ISSRs is more specific, and therefore more reliable. This study is the first to study genetic relationships among all species studied here using biochemical and molecular techniques.

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“…The genus Phlomis L. contains over 100 species [8], distributed throughout Europe, Africa, and Asia. Many of them have aromatic and medicinal characteristics [9].…”

Section: Introductionmentioning

confidence: 99%

Glandular and Non-Glandular Trichomes from Phlomis herba-venti subsp. pungens Leaves: Light, Confocal, and Scanning Electron Microscopy and Histochemistry of the Secretory Products

Gostin

2023

Plants

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The purpose of this paper is to highlight the morphological peculiarities of glandular and non-glandular trichomes from leaves of Phlomis herba-venti subsp. Pungens using light, confocal, and scanning electron microscopy. Histochemistry techniques were used to analyze the localization of different chemical compounds in secretory trichomes. Two types of non-glandular trichomes were identified: unicellular and branched. They were found more frequently on the lower epidermis of leaves in different stages of ontogenetic development. Glandular trichomes were categorized as capitate (C1 and C2) with different stalk lengths and one–four secretory cells and dendroids (D) with one–four secretory cells. The histochemical analyses revealed distinct secretory products in terms of composition and distribution among the three types of glandular trichomes. The dendroid category of glandular trichomes is rarely found in plants and is not characteristic of the Lamiaceae species. They were described and characterized from a micromorphological and histochemical point of view for the first time in P. herba-venti.

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Estimation of phylogenetic relationships of Phlomis species based on seed protein polymorphism

Cited by 5 publications

References 19 publications

Comparative proteomic analysis of Orobanche and Phelipanche species inferred from seed proteins

Comparative proteomic analysis of Orobanche and Phelipanche species inferred from seed proteins

Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

Glandular and Non-Glandular Trichomes from Phlomis herba-venti subsp. pungens Leaves: Light, Confocal, and Scanning Electron Microscopy and Histochemistry of the Secretory Products

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