Estimation of ploidy levels by flow cytometry and identification of Torreya grandis cultivars and breeding strains by SSR markers

Youwu, Chen; Ye, Bihuan; Qin, Song; Pu, Weiting; Shen, Jianjun; Li, Haibo

doi:10.1007/s00468-022-02324-x

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Till date, 11 kitaviral species were recognized by International Committee on Taxonomy of Viruses (ICTV) (https://ictv.global/taxonomy) while a few new kitaviruses ( 7) are yet to be recognized (Ramos-González et al, 2023). Among the genera, Cilevirus has the maximum number of recognized species (7) that are characterized at the molecular and biological level followed by Blunervirus (3) and Higrevirus (1). Limited information on intraspecies-level genomic variability of kitaviruses is a constraint for establishing sequence identity based species demarcation criteria for kitaviruses (Ramos-González et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

2.5-fold expansion of the genetic diversity of blunerviruses through plant (meta)transcriptome data-mining

Reddy,

Sidharthan

2024

Preprint

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Kitaviruses are plant-infecting, non-systemic disease-causing viruses with multipartite single-stranded RNA genomes. Despite their importance, knowledge on kitaviruses is limited in comparison with other plant virus groups, mainly because of the lesser number of identified and characterized kitaviruses and their isolates. In the present study, we explored plant (meta)transcriptome data available in public domain and identified genome sequences of fifteen putative novel blunerviruses in fifteen plant species, including three gymnospermic and three monocot species. Four RNA segments (RNAs 1–4) of ten identified viruses were recovered, whilst atleast two RNA segments were recovered for the remaining viruses. Monocistronic RNA1 of identified viruses encodes methyltransferase (MET)-helicase (HEL) polyprotein, except RNA1 of Nanmu blunervirus 1 that encodes a relatively larger MET-HEL-RNA-dependent RNA polymerase (RdRp) polyprotein. Monocistronic RNAs 2 and 4, respectively of identified viruses, encode HEL-RdRp polyprotein and movement protein (MP). Three to four open reading frames (ORFs) were determined in RNA3 of identified viruses, of which the relatively conserved ORF3, always encodes the negevirus membrane protein (SP24) homolog. Phylogenetic analysis grouped the identified viruses with known blunerviruses. Based on genome organization, sequence identities of encoded proteins with known blunerviruses and phylogeny, the identified viruses are regarded as new members of the genus Blunervirus. The study paves way for initiating further studies on understanding the biological properties, economic importance and geographical distribution of identified blunerviruses.

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Section: Introductionmentioning

confidence: 99%

2.5-fold expansion of the genetic diversity of blunerviruses through plant (meta)transcriptome data-mining

Reddy,

Sidharthan

2024

Preprint

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Establishment and Optimization of an Experiment System for Flow Cytometry in Oil-Seed Camellia

Zhang,

Wang

et al. 2024

Horticulturae

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Ploidy identification is a basic method for determining germplasm resources and for breeding new varieties of oil-seed camellia. In this study, flow cytometry and K-mer analysis were used to identify the ploidy of oil-seed camellia germplasms. To determine the best tissue organ type, lysis time, and dyeing time, evaluation indices such as the presence of a clear main peak, the ease of sampling, and the coefficient of variation were used. A technique was established, and the ploidies of different oil-seed camellia germplasms were identified. The results showed that pollen was the best material and that using a 400 mL PI lysis solution for 10 min lysis, followed by dyeing with a 1600 mL DAPI dyeing solution for 10 min, was the most suitable technique. According to the peak value of the control diploid Camellia azalea, 15 samples were estimated to be diploid, 24 samples were tetraploid, 18 samples were hexaploid, and 13 samples were octoploid. In addition, the K-mer analysis results showed that among the five samples, CK, C051, and C047 were diploid, while C037 and C043 were tetraploid, results that are consistent with the results of the flow cytometry identification. This study is therefore valuable for the polyploid selection and use of different ploidy germplasm resources for the cross breeding of oil-seed camellia.

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Estimation of ploidy levels by flow cytometry and identification of Torreya grandis cultivars and breeding strains by SSR markers

Cited by 2 publications

References 15 publications

2.5-fold expansion of the genetic diversity of blunerviruses through plant (meta)transcriptome data-mining

2.5-fold expansion of the genetic diversity of blunerviruses through plant (meta)transcriptome data-mining

Establishment and Optimization of an Experiment System for Flow Cytometry in Oil-Seed Camellia

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