Estimation of Pollen Contamination and Mating System in Pinus Merkusii Seedling Seed Orchard Using Allozyme Markers

Suwarni, Endah; Omondi, Stephen F.; Bp, Dancik; Khasa, Damase P.

doi:10.26525/jtfs2018.30.1.95105

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…These estimates can be generated by examining allelic variation over many loci in progeny arrays from plants allowed to pollinate naturally, using a maximum-likelihood model to exclude progeny resulting from self-fertilization (Ritland and Jain, 1981). Outcrossing rates in plant populations have often performed using codominant markers such as SSR, allozyme (Chen et al, 2009;Suwarni et al, 2018). However, in the absence of sufficient allozyme polymorphism, several dominant markers such as RAPD (Gaiotto et al, 1997;Dong et al, 2006), ISSR (Han et al, 2009;Fernando et al, 2015;Saki et al, 2016), and AFLP (Gaiotto et al, 1997;Pometti et al, 2013., Zhao et al, 2014Dong et al, 2018a;2018b)…”

Section: Introductionmentioning

confidence: 99%

Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers

Dong

Liao

et al. 2018

Not Bot Horti Agrobo

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In this study, mating system, genetic diversity, and genetic structure of the endangered endemic aquatic Isoetes yunguiensis in China was investigated using ISSR markers. The results of ISSR analyses showed that the estimate of multilocus outcrossing rate (tm) was high at species level (tm = 0.955), indicating that diploid I. yunguiensis is a predominant outcrossing species. Nine selected ISSR primers used in the study amplified 66 reproducible bands, 41 of which were polymorphic among 37 individuals. High level of genetic diversity was detected at the species level (PPB = 62.12%), whereas, relatively low genetic diversity existed within populations (PPB = 39.39%). Analysis of molecular variance (AMOVA) revealed that 31.99% of the genetic variation was attributable to differences between populations and the rest (68.01%) to variability within populationsof I. yunguiensis. Value of Fst (0.320) indicated that genetic differentiation between populations also was significant. These results showed that I. yunguiensis predominantly favors crossing, and has a high level of genetic diversity and highly significant genetic variation between and within populations. Gene flow (Nm) among populations is equal to 1.177. High outcrossing rates may be responsible for the high levels of genetic diversity observed in the I. yunguiensis population. To maintain the current level of genetic diversity for this species, we recommend increasing in situ conservation sites.

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Section: Introductionmentioning

confidence: 99%

Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers

Dong

Liao

et al. 2018

Not Bot Horti Agrobo

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“…Inferences about plant mating systems increasingly use highly informative genetic markers and investigate finer facets of the mating system (Ritland, 2002). In recent studies, although several dominant markers, such as RAPD (Gaiotto et al, 1997;Dong et al, 2006), ISSR (Han et al, 2009;Fernando et al, 2015;Saki et al, 2016), and AFLP (Gaiotto et al, 1997;Pometti et al, 2013;Zhao et al, 2014;Dong et al, 2018 b) markers, have been used to estimate outcrossing rates in plant species, outcrossing rates in plant populations have often been determined using codominant markers, such as isozymes and microsatellites, due to their high allelic polymorphism (Chaix et al, 2003;Chen et al, 2009;Adugna et al, 2013;Sinha et al, 2015;Sharma et al, 2017;Suwarni et al, 2018;Zhang et al, 2019;Dauphin et al, 2020;Sun et al, 2020;Torres et al, 2020;Calevo et al, 2021;Heike and Dagmar, 2022;Lu et al, 2022).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Outcrossing mating system in natural populations of the endangered aquatic fern Ceratopteris thalictroides in China as revealed by microsatellite markers: implications for conservation

DONG,

SONG,

BAI

et al. 2024

Not Bot Horti Agrobo

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In this study, the mating system of the endangered aquatic fern Ceratopteris thalictroides in China was investigated using microsatellite markers. The estimates of the multilocus outcrossing rate (tm) and single-locus outcrossing rate (ts) were high at the species level (tm = 0.701 and ts = 0.595, respectively), indicating that C. thalictroides is a predominantly outcrossing species. Negative F (inbreeding coefficient of the maternal parents) values (-0.137) at the species level indicated an excess of heterozygotes and low inbreeding in the populations analyzed. Our investigation revealed that some of the natural and ex situ conservation populations of C. thalictroides have a prolific capacity for vegetative growth. The results showed that the homosporous pteridophyte C. thalictroides in China predominantly favors gametophytic crossing with a certain level of inbreeding along with vegetative growth. Ten populations have been established for ex situ conservation in Wuhan, China, with a survival rate of more than 90%, and mature spores were collected from them. Several factors, including high dispersal of spores and gametophytes by flowing water, high gene flow, and the wide distribution of the species in China, with the occurrence of some large populations, may have resulted in a high outcrossing rate in Chinese C. thalictroides. The high outcrossing rate of C. thalictroides showed that ex situ plantings are most suitable for enhancing possible mixing of the different populations, thus minimizing inbreeding.

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Estimation of Pollen Contamination and Mating System in Pinus Merkusii Seedling Seed Orchard Using Allozyme Markers

Cited by 2 publications

References 14 publications

Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers

Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers

Outcrossing mating system in natural populations of the endangered aquatic fern Ceratopteris thalictroides in China as revealed by microsatellite markers: implications for conservation

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