Estimation of Postmortem Interval Using Kinetic Analysis of the Third Component of Complement (C3) Cleavage

Kominato, Yoshihiko; Kumada, Kaoru; Yamazaki, Kentarou; Misawa, Shogo

doi:10.1520/jfs12623j

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Previous studies also showed that proteins in tissues and serum were degraded due to catabolic metabolism. 1,9,10,[13][14][15]37,38 Many proteolytic enzymes can be released from the lysosome during cell autolysis. The reduction of the absorption area of amide I confirms that ATR spectra also show the degradation of proteins after death.…”

Section: Resultsmentioning

confidence: 99%

Attenuated Total Reflection Fourier Transform Infrared Spectroscopic Investigation of the Postmortem Metabolic Process in Rat and Human Kidney Cortex

Tuo

Huang

et al. 2010

Appl Spectrosc

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Attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectroscopy has been applied to study the short and long term postmortem metabolic processes in rat and human kidney cortexes. The goals of this project were as follows: (1) to investigate the changes of ATR spectra in different rat and human tissues after death, (2) to explore the best mathematical model with different band absorption ratio changes to determine the postmortem interval (PMI), and (3) to establish a preliminary human postmortem ATR spectra database. There were three different types of metabolic changes after death based on the spectral results: (1) the intensities of some bands increased continuously (e.g., C-H stretching region), (2) the intensities of other bands decreased continuously (e.g., PO(2)(-) symmetric stretching), and (3) other bands remained relatively stable (e.g., C-OH bending, CO-O-C antisymmetric stretching). The band absorbance ratios for rats were found to display either a significant increase or decrease with increasing time after death. Of the absorbance ratios of the various bands investigated to find the best fit with the cubic model function in rats, the A(1652)/A(1396) ratio showed the strongest correlation (R(2) = 0.937). Comparison of the rat kidney cortex spectra with selected human postmortem cases showed similar postmortem metabolic changes. In conclusion, ATR FT-IR spectroscopy was shown to be a convenient and reliable method of determining short and long term postmortem intervals by simultaneously monitoring several specific parameters, although these observations have yet to be applied at forensic scenes by further field studies.

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Section: Resultsmentioning

confidence: 99%

Attenuated Total Reflection Fourier Transform Infrared Spectroscopic Investigation of the Postmortem Metabolic Process in Rat and Human Kidney Cortex

Tuo

Huang

et al. 2010

Appl Spectrosc

View full text Add to dashboard Cite

show abstract

“…A method has been described for the rapid HPLC analysis of a number of anticonvulsant drugs in vitreous humor by injection directly onto a preconcentration column without the need for prior extraction (305). A comparison was given of chemical methods for determining postmortem interval (306) and the estimation of postmortem interval by using kinetic analysis of the third component of complement (C3) cleavage (307).…”

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confidence: 99%