Estimation of Primary and Secondary Metabolites From Leaves of Three Medicinal Plants

Abstract

Medicinal plants areimportant source of life saving drugs for majority of the world population. The study includes phytochemical screening and quantification of primary and secondary metabolites like chlorophyll, carbohydrates, protein, phenol, starch and ascorbic acid fromleaves extract of selected plants namely: Chenopodium album, Moringa oleifera and Terminalia arjuna. The highest amount of protein (75.46 μg/ml) was observed in T. arjuna, ascorbic acid (38.02 mg/g dw), starch (5.12 mg/g dw), phenols (22.55 mg/g dw) and chlorophyll (14.75 mg/g dw) was observed in leaves of C. album.

Ascorbic Acid (Antioxidant) in Aegle Marmelos and Moringa Oleifera and Effect of Growth Regulators and Salts on it in Vitro

“…Free endogenous ascorbic acid production has been reported in tissue culture of Momordica charantia and Emblica officinalis (Mohan et al,1974), Datura metel and Datura tatula , Trigonella foenum-graceum ( Jain et al, 1975 ), Ephedra foliata, Helianthus annus, Agave wightii and Tephrosia purpurea (Khanna et al, 1977) ; Solanum xanthocarpum (Manot, 1977), Atropa belladona (Sharma, 1977), Papaver somniferum (Gaur, 1978, Khanna et al, 1977, Daucus carota (Sogani, 1978), Solanum nigrum (Rathore et al, 1979), Tribulus alatus and Zygophyllum simplex (Jit et al, 1986), Lycium bararum (Nag and Grover, 1987),Eclipta alba (Mathur,1988) Seetzenta orientatis (Sethia,1988), Calligonum polygonoides and Lasiurus sindicus (Bhojak,1991), Tinospora cordifolia (Goswami and Yadav,1994),Lycium barbarum (Mukhi, 1995), Peganum harmala (Badia, 1999), Arabidopsis cell suspension cultures (Davery et al, 1999), Ribes nigrum (Viola et al, 2000),Vigna aconitifolia (Tyagi, 2002),Capparis decidua and Ziziphus sp. (Chauhan, 2003),Cassia angustifolia (Reddy, 2005), Balanitis aegyptiaca (Bedawat, 2006), Ailanthus excelsa (Rao, 2007), Adhatoda vasica and Barleria prionitis (Deepa, 2009), Cocculus pendulus and Tinospora cordifolia (Yadav, 2010) Licensed Under Creative Commons Attribution CC BY (Sharma,2012)., three medicinal plants (Khandelwal et al, 2014). These were then centrifuged separately at low speed (2500 rpm) for fifteen minutes, the residues were discarded and the supernatants were used for the estimation of ascorbic acid following the procedure of Jensen (1962).…”