Estimation of reactogenicity of preparations produced on the basis of photoinactivated live vaccines against brucellosis and tularaemia on the organismic level. 1. Using the LASCA method

Ulianova, Onega V.; Uianov, S S; Li, Pengcheng; Luo, Qingming

doi:10.1070/qe2011v041n04abeh014598

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…5E), with concentration 5 to 500 ppm were inactivated when illuminated with a 650 nm LED. 181 B. abortus and F. tularensis were illuminated with 650 nm LED and saline and no killing effect was observed eliminating the possibility of photothermal damage. 181 Recently, Y. pestis, a gram-negative bacterium, has gained attention as a possible biological warfare agent.…”

Section: Photodynamic Inactivation (Pdi) Of Biological Warfare Agentsmentioning

confidence: 99%

“…181 B. abortus and F. tularensis were illuminated with 650 nm LED and saline and no killing effect was observed eliminating the possibility of photothermal damage. 181 Recently, Y. pestis, a gram-negative bacterium, has gained attention as a possible biological warfare agent. A possible surrogate to study photoinactivation of Y. pestis is the gram-negative bacterium Y. enterocolitica.…”

Section: Photodynamic Inactivation (Pdi) Of Biological Warfare Agentsmentioning

confidence: 99%

“… 181 B. abortus and F. tularensis were illuminated with 650 nm LED and saline and no killing effect was observed eliminating the possibility of photothermal damage. 181 …”

Section: Photodynamic Inactivation (Pdi) Of Biological Warfare Agentsmentioning

confidence: 99%

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Can biowarfare agents be defeated with light?

et al. 2013

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Biological warfare and bioterrorism is an unpleasant fact of 21st century life. Highly infectious and profoundly virulent diseases may be caused in combat personnel or in civilian populations by the appropriate dissemination of viruses, bacteria, spores, fungi, or toxins. Dissemination may be airborne, waterborne, or by contamination of food or surfaces. Countermeasures may be directed toward destroying or neutralizing the agents outside the body before infection has taken place, by destroying the agents once they have entered the body before the disease has fully developed, or by immunizing susceptible populations against the effects. A range of light-based technologies may have a role to play in biodefense countermeasures. Germicidal UV (UVC) is exceptionally active in destroying a wide range of viruses and microbial cells, and recent data suggests that UVC has high selectivity over host mammalian cells and tissues. Two UVA mediated approaches may also have roles to play; one where UVA is combined with titanium dioxide nanoparticles in a process called photocatalysis, and a second where UVA is combined with psoralens (PUVA) to produce “killed but metabolically active” microbial cells that may be particularly suitable for vaccines. Many microbial cells are surprisingly sensitive to blue light alone, and blue light can effectively destroy bacteria, fungi, and Bacillus spores and can treat wound infections. The combination of photosensitizing dyes such as porphyrins or phenothiaziniums and red light is called photodynamic therapy (PDT) or photoinactivation, and this approach cannot only kill bacteria, spores, and fungi, but also inactivate viruses and toxins. Many reports have highlighted the ability of PDT to treat infections and stimulate the host immune system. Finally pulsed (femtosecond) high power lasers have been used to inactivate pathogens with some degree of selectivity. We have pointed to some of the ways light-based technology may be used to defeat biological warfare in the future.

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Section: Photodynamic Inactivation (Pdi) Of Biological Warfare Agentsmentioning

confidence: 99%

Section: Photodynamic Inactivation (Pdi) Of Biological Warfare Agentsmentioning

confidence: 99%

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Can biowarfare agents be defeated with light?

et al. 2013

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“…Such method of LASCA [ 15 ] was proposed more than 20 years ago. Previously, this method was used to diagnose the blood flow in normal state and under some pathological changes [ 16 , 17 ], to monitor of malignant tumor growth, to test the toxicity of the new-generation vaccine against very dangerous diseases [ 18 ], and to study the hydrodynamics of microflow in isolated blood vessel in the mesentery of white rats [ 19 ]. Relatively recently, essential progress has been achieved in the implementation of LASCA method to monitoring bacterial colony growth [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Could LASCA-imaging of GB-speckles be applied for a high discrimination and typing of pathogenic bacteria?

Ulianova

Ulyanov

Zaytsev³

et al. 2021

PLoS ONE

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In this article, the method of analysis of GB-speckles (gene-based speckles) has been adapted to the problem of detecting the differences in a group of genes (usually 5–7), used in Multi Locus Sequence Typing (MLST). This method is based on s-LASCA imaging (spatial Laser Speckle Contrast Analysis) of virtual GB-speckle and on the technique of RGB coordinates for GB-speckles, processed by the s-LASCA method. A very high sensitivity and accuracy of the new method for detecting gene polymorphism as a great alternative to classical MLST has been demonstrated. The analysis of GB-speckles, obtained for the concatenated sequences of seven genes (gatA, gidA, enoA, fumC, hemN, hflX, oppA) of three different Chlamydia trachomatis strains (E/Bour, ST94; G/9301, ST95; G/11222, ST94) has been applied as the model. The high efficiency of usage of s-LASCA-imaging of GB-speckles has been shown. The data obtained represent a significant progress in digital biology as a whole and improvements in the bio-digitalization of bacterial DNA.

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“…The method of LASCA [9,10] was proposed more than 20 years ago. Previously, this method has been used in the diagnosis of blood flow in either norm or for some pathological changes [11][12][13], for monitoring of the growth of malignant tumors, for testing the toxicity of new-generation vaccines against especially dangerous diseases [14], and in studying the hydrodynamics of microflow in isolated blood vessels in mesentery of white rats [15]. Recently, essential progress has been achieved with implementation of LASCA in monitoring the growth of bacterial colonies [16].…”

Section: Introductionmentioning

confidence: 99%

LASCA-imaging of gene-based speckles: application to detection of the gene polymorphism in a bacterial model

Ulianova

Ulyanov²,

Zaytsev³

et al. 2020

Laser Phys. Lett.

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A current trend in modern bioinformatics is representation of nucleotide sequences in the form of gene-based (GB) speckles. In this letter GB-speckles have been processed using the s-LASCA (laser speckle contrast analysis) technique. Statistical properties of s-LASCA images of GB-speckles have been analyzed. It is shown that application of the s-LASCA technique allows us to essentially increase the the sensitivity of detection of a single nucleotide polymorphism using GB-speckles.

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Estimation of reactogenicity of preparations produced on the basis of photoinactivated live vaccines against brucellosis and tularaemia on the organismic level. 1. Using the LASCA method

Cited by 10 publications

References 13 publications

Can biowarfare agents be defeated with light?

Can biowarfare agents be defeated with light?

Could LASCA-imaging of GB-speckles be applied for a high discrimination and typing of pathogenic bacteria?

LASCA-imaging of gene-based speckles: application to detection of the gene polymorphism in a bacterial model

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