To control the water pollution in the Pearl River Estuary (PRE), a series of measures have been enacted in recent years. The efficacy of these measures on water quality improvement is, however, currently unknown. To evaluate the variation of water quality in response to the pollution control measures in the PRE during the last decade (2008)(2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017), our study conducted a long-term monitoring program of estuarine water in the representative city Guangzhou that targeted fecal coliform (F. Coli), biochemical oxygen demand (BOD 5 ), chemical oxygen demand (COD Cr ), potassium permanganate index (COD Mn ), petroleum, total nitrogen (TN), ammonia nitrogen (NH 3 -N) and total phosphorus (TP). In the last decade, F. Coli, BOD 5 , COD Cr and COD Mn , petroleum and NH 3 -N have shown a significant reduction by 78.8%, 50.9%, 37.5%, 18.9%, 75.0% and 25.0%, respectively. In contrast, TN and TP remained stable. Water quality index calculations indicated that the water quality was elevated from the marginal-fair level to the good level, particularly after 2012. The biochemical pollutants and nutrients in the estuarine water most likely originated from the upper river due to the wastewater discharge, fecal pollution and agricultural input. The success of pollutant reduction could thus be attributed to industrial upgrading and relocation, as well as the improvement of the sewage treatment system in Guangzhou. However, efficient approaches to reduce TN pollution should be implemented in the future.