Estimation of serum malondialdehyde and assessment of DNA damage using comet assay in patients with oral submucous fibrosis

Paulose, Swetha; Rangdhol, Vishwanath; Ramesh, R.; Jeelani, S; Brooklyin, S

doi:10.1111/jicd.12153

Cited by 10 publications

(11 citation statements)

References 27 publications

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“…showed higher serum MDA levels and DNA damages in 30 OSF patients than in 30 healthy control subjects. 28 Hence, MDA, an oxidative biomarker, together with comet assay analysis to detect DNA damage may be of diagnostic value to identify OSF patients with high risk of malignant potential. 28 …”

Section: Discussionmentioning

confidence: 99%

“… 20 In recent years, more and more associated biomarkers have been proved to be related to OSF. 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 The biomarkers involved in OSF can be further categorized into solid tissue markers, serum markers, and saliva markers. Tissue markers associated with OSF include proliferating cell nuclear antigen (PCNA), Ki67, hypoxia-inducible factor 1-alpha (HIF-1α), E-cadherin, Shh, Gli-1, CD1a, CD207, bax, and p53, which are involved in cell proliferation, hypoxia, epithelial–mesenchymal transition, immunity, tumor suppressor genes, cell apoptosis, and angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…These markers are involved in antioxidant activity, oxidative stress, and cell metabolism, respectively. 26 , 27 , 28 , 29 , 30 The number of serum and saliva markers is lower than the number of the solid tissue markers. However, for clinical use, the acquisition of the sera or saliva for marker detection is relatively convenient and accepted by the patients.…”

Section: Introductionmentioning

confidence: 99%

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Significantly higher serum tumor marker levels in patients with oral submucous fibrosis

Lin

Yang

et al. 2021

Journal of Dental Sciences

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Background/purpose Our previous study showed that carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC–Ag), and ferritin are significantly higher in patients with oral potentially malignant disorders (OPMDs including oral leukoplakia, oral erythroleukoplakia, and oral verrucous hyperplasia) than in healthy controls (HCs). Oral submucous fibrosis (OSF) is also recognized as an OPMD. This study evaluated whether these three serum tumor marker levels were also significantly higher in OSF patients than in HCs. Materials and methods The serum CEA, SCC-Ag, and ferritin levels in 41 OSF patients and 164 HCs were measured and compared. Patients with serum CEA level ≥3 ng/mL, SCC-Ag level ≥2 ng/mL, and ferritin level ≥250 ng/mL were scored as serum positive for CEA, SCC-Ag, and ferritin, respectively. Results We found significantly higher mean serum CEA, SCC-Ag, and ferritin levels in 41 OSF patients than in 164 HCs (all P -values < 0.05). Moreover, 41 OSF patients had significantly higher serum positive rates of CEA (39.0%), SCC-Ag (19.5%), and ferritin (53.7%) than 164 HCs (all P -values < 0.05). Of the 41 OSF patients, 26 (63.4%), 7 (17.1%), and 2 (4.9%) had serum positivities of one, two, or three tumor markers including CEA, SCC-Ag, and ferritin, respectively. Conclusion There are significantly higher mean serum CEA, SCC-Ag, and ferritin levels and significantly higher serum positive rates of CEA, SCC-Ag, and ferritin in OSF patients than in HCs. The serum CEA, SCC-Ag, and ferritin levels may be served as tumor markers for evaluation of malignant potential of OSF lesions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Significantly higher serum tumor marker levels in patients with oral submucous fibrosis

Lin

Yang

et al. 2021

Journal of Dental Sciences

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“…Serum nitric oxide (NO) was determined using the Griess method (Invitrogen, CA, USA). Serum malondialdehyde (MDA) was measured using the thiobarbituric acid-trichloroacetic acid (TBA-TCA) method [ 27 ]. Serum superoxide dismutase (SOD) activity was analyzed by using a SOD kit (Nanjing Jiancheng Bioengineering Institute).…”

Section: Methodsmentioning

confidence: 99%

Antarctic Krill Oil Attenuates Oxidative Stress via the KEAP1‐NRF2 Signaling in Patients with Coronary Heart Disease

Cui

Jiang

Huang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Antarctic krill oil (AKO) has strong antioxidant activities and is effective for alleviating coronary heart disease (CHD). Kelch-like ECH-associated protein 1-NF-E2-related factor 2 (KEAP1-NRF2) axis is a crucial antioxidant signaling pathway. Thus, AKO may exert its antioxidant effects on CHD patients via KEAP1-NRF2 signaling. Methods. AKO fatty acid (FA) profiles were analyzed by using gas chromatography (GC). One hundred CHD patients were divided into the intervention (IG, AKO) and control (CG, placebo) groups. Before and after 1, 2, and 3 months of intervention, we measured serum levels of reactive oxygen species (ROS), 8-hydroxy-2-deoxyguanosine (8-OHdG), nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GPx), and KEAP1 and NRF2 levels in peripheral blood leukocytes (PBLs). Serum FAs were measured by GC at baseline and after 3-month intervention. Results. AKO contains rich eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which is more than 27% of total FA. The levels of EPA and DHA, KEAP1, and NRF2 in the IG group were higher than those in the CG group ( p < 0.05 ). Serum levels of ROS, 8-OHdG, NO, and MDA in the IG group were lower than those in the CG group, whereas the levels of SOD, GSH, and GPx in the IG group were higher than those in the CG group ( p < 0.05 ). Serum levels of saturated fatty acids (UFA) in the IG group were higher than those in the CG group, whereas reverse results were obtained for the levels of saturated fatty acids (SFA). Serum levels of EPA and DHA had a strong negative relationship with the level of ROS, whereas the ROS level had a strong negative relationship with the levels of KEAP1-NRF2. Conclusion. AKO increases antioxidant capacities of CHD patients via the KEAP1-NRF2 signaling in the PBL.

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“…OSF patients presented with elevated DNA damage and lipid peroxidation levels compared with healthy controls. As malondialdehyde (MDA) is a lipid peroxidation marker, the evaluation of its levels by comet assay may help identify OSF patients with high malignant potential [98].…”

Section: Biomarkers In Osf Serummentioning

confidence: 99%

Oral Submucous Fibrosis: A Review on Etiopathogenesis, Diagnosis, and Therapy

Shih

Wang

Shieh

et al. 2019

IJMS

174

111

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Oral submucous fibrosis (OSF) is characterized by abnormal collagen deposition. It is a precancerous disorder and transforms into a malignant tumor in 1.5–15% of all cases. Symptoms include submucous fibrosis, ulceration, xerostomia, a burning sensation, and restricted mouth opening. All of these greatly interfere with patient quality of life. The present review introduces OSF from a molecular perspective and summarizes what is known about its underlying mechanisms, diagnostic biomarkers, and therapeutic interventions. In addition to the aggressive treatment of OSF, its prevention is also important. Future research should, therefore, focus on improving the oral health literacy of the patients susceptible to OSF.

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Estimation of serum malondialdehyde and assessment of DNA damage using comet assay in patients with oral submucous fibrosis

Cited by 10 publications

References 27 publications

Significantly higher serum tumor marker levels in patients with oral submucous fibrosis

Significantly higher serum tumor marker levels in patients with oral submucous fibrosis

Antarctic Krill Oil Attenuates Oxidative Stress via the KEAP1‐NRF2 Signaling in Patients with Coronary Heart Disease

Oral Submucous Fibrosis: A Review on Etiopathogenesis, Diagnosis, and Therapy

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