Estimation of serum total lipids

Woodman, Dorothy; Price, Christopher P.

doi:10.1016/0009-8981(72)90205-7

Cited by 75 publications

(15 citation statements)

References 3 publications

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“…Serum level of a-amino acids was measured according to Matthews et al (1964). Serum total lipid was estimated as described by Woodman & Price (1972). Serum albumin, globulin, ammonia, glucose and cholesterol concentrations were measured using kits ( (1974) respectively.…”

Section: Determination Of Serum Ions and Metabolitesmentioning

confidence: 99%

Influence of oral administration of 3,5,3′‐triiodo‐thyronine on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major (Temminck & Schlegel)

Woo

Chung

1991

Journal of Fish Biology

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The effect of inclusion of 3,5,3'-triiodo-~-thyronine (T,) in the diet was examined in underyearling red sea bream Chrysophrys major (Temminck 8c Schlegel). The treatment brought about increases in growth rate, appetite, food conversion efficiency and activities of intestinal enzymes including leucine nitroanilidase, alkaline phosphatase, y-glutamyltransferas, a-amylase and disaccharidase. There were no changes in the muscle content of water, protein, lipid and glycogen. Liver glycogen content was elevated, as well as activities of the hepatic enzymes glycogen phosphorylase, glycogen synthetase, glutamate-pyruvate transaminase, fructose-1.6-diphosphatase and glucose-6-phosphatase. The serum concentrations of total protein, albumin, globulin, u-amino acids. glucose, ammonia and calcium were increased by the treatment whereas the serum concentrations of free fatty acids, cholesterol and triglyceride remained unaltered. The results suggest that in the red sea bream T, stimulated protein and carbohydrate but not lipid metabolism and that the hormone promoted growth by improving appetite, digestion and absorption.

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Section: Determination Of Serum Ions and Metabolitesmentioning

confidence: 99%

Influence of oral administration of 3,5,3′‐triiodo‐thyronine on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major (Temminck & Schlegel)

Woo

Chung

1991

Journal of Fish Biology

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“…Serum glucose level was measured by a coupled glucose oxidase-peroxidase reaction (Sigma Bulletin 510). Estimation of serum total lipid was performed using a sulphophosphovanillin reaction (Woodman and Price 1972). Determination of serum total cholesterol involved the use of the enzymes cholesterol esterase, cholesterol oxidase and peroxidase (Sigma Bulletin 351).…”

Section: Determination Of Serum Ions and Metabolitesmentioning

confidence: 99%

“…Tissue lipid was extracted in chloroform/methanol (2:1 v/v) using the method of Folch et al (1957). Total tissue lipid was determined on aliquots of lipid extracts according to Woodman and Price (1972). Tissue samples were digested in 5 M NaOH and the protein contents determined according to Hartree (1972).…”

Section: Tissue Metabolite Analysesmentioning

confidence: 99%

Influence of oral administration of estradiol-17? and testosterone on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major

Woo

Chung

1993

Fish Physiol Biochem

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Estradiol-17β (E2) administered in the diet to the red sea bream Chrysophrys major did not affect appetite, food conversion efficiency, protein efficiency ratio and specific growth rate. Serum concentrations of total protein, albumin, globulin, vitellogenin, α-amino acids, total lipid, free fatty acids, cholesterol and calcium were elevated. The hepatosomatic index was also increased. Activities of hepatic enzymes including lactate dehydrogenase, isocitrate dehydrogenase and glutamate-pyruvate transaminase were higher than found in untreated control fish. Intestinal activity of leucine aminopeptidase was augmented. However, there were no changes in muscle water, protein, lipid and glycogen content. In contrast, testosterone (T) given by the same route increased appetite, food conversion efficiency, protein efficiency ratio and specific growth rate. There were no alterations in serum protein and calcium concentrations but serum glucose, ammonia and triglyceride levels were elevated. Hepatic glycogen content was increased. The activities of hepatic fructose- 1,6-diphosphatase, glucose-6-phosphatase and glycogen synthetase and intestinal activities of alkaline phosphatase and τ-glutamyltransferase were higher than noted on control fish. The results reveal that estradiol-17β and testosterone exerted different metabolic effects in the red sea bream and they suggest that testosterone exerts its anabolic actions by increasing appetite, food conversion efficiency and activities of digestive enzymes.

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“…Triglycerides were measured on an autoanalyser using the method of Noble and Campbell (1970) as modified by McLellan (1971). Total lipids were measured by the method of Woodman and Price (1972). Lipoprotein electrophoresis was performed on cellulose acetate which was stained with 'Oil red 0' (Dangerfield and Tarlow, 1971).…”

Section: Methodsmentioning

confidence: 99%

Blood lipids and the treatment of essential hypertension with methyldopa and bendrofluazide

Libman

Dormandy

Arrowsmith

et al. 1974

Postgraduate Medical Journal

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Summary The blood lipids of eight patients with previously untreated essential hypertension have been studied before and after treatment with methyldopa and bendrofluazide. No significant change was found in any of the measurements over a mean treatment period of 5·25 months. These results are believed to provide evidence that hypotensive therapy does not predispose to the development of ischaemic heart disease.

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Estimation of serum total lipids

Cited by 75 publications

References 3 publications

Influence of oral administration of 3,5,3′‐triiodo‐thyronine on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major (Temminck & Schlegel)

Influence of oral administration of 3,5,3′‐triiodo‐thyronine on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major (Temminck & Schlegel)

Influence of oral administration of estradiol-17? and testosterone on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major

Blood lipids and the treatment of essential hypertension with methyldopa and bendrofluazide

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