Estimation of serum α2-macroglobulin based on the esterolytic activity of bound α-chymotrypsin

Rao, N.Ramalingeshwara; Bhat, Pallavi; Pattabiraman, T. N.

doi:10.1016/0006-2944(84)90042-5

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…A2MG activity of heparinized human plasma was estimated based on the chymotrypsin-bound esterase (CTBE) activity as described by Rao et al (1984) with modifications.…”

Section: Methodsmentioning

confidence: 99%

Augmented rescue of macroglobulins by supplementation of anti-snake venom with methanolic extract of Andrographis paniculata in Naja naja envenomation

et al. 2022

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Proteins of the macroglobulin family are prime targets of venom enzymes in snake bite. A massive reduction in the active concentration of these multifunctional proteins in snake bite, makes the living system vulnerable to dysregulation. This study investigates the ability of Indian polyvalent anti-snake venom (ASV), methanolic extract of Andrographis paniculata (MAP) and their combination in rescuing human alpha 2-macroglobulin (A2MG) and its homologues in rat plasma, from inactivation by Naja naja (N.N) venom enzymes. In-vitro experiments were conducted with heparinized human plasma and in-vivo experiments with female Wistar rats. Along with appropriate controls, there were 3 test groups in in-vitro and 8 test groups in in-vivo experiments. The in-vitro test groups were exposed to N.N venom for zero, 30 or 90 min prior to incubation with ASV or MAP or reduced ASV supplemented with MAP and incubated for 16 h at 37 °C. Chymotrypsin-bound esterase (CTBE) activity of A2MG was estimated. Rats were administered the venom intramuscularly and treated with ASV/MAP/ASV + MAP. CTBE activity of macroglobulin homologues was measured on day 1, 7 and 14. Survival of animals was noted. In human plasma, addition of ASV or MAP or ASV + MAP prevented loss of A2MG activity maximally to the extent of 88–100% (p = 0.001). In rats, reduced concentration of ASV supplemented with MAP showed complete rescue of macroglobulin homologues and 90% survival. The compulsive evidence from this study, underscores the merits of using this multipronged strategy in rescuing the macroglobulins and improving survival in envenomation due to N.N.

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“…A2MG activity of heparinized human plasma was estimated based on the chymotrypsin-bound esterase (CTBE) activity as described by Rao et al (1984) with modifications.…”

Section: Methodsmentioning

confidence: 99%

Augmented rescue of macroglobulins by supplementation of anti-snake venom with methanolic extract of Andrographis paniculata in Naja naja envenomation

et al. 2022

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“…One unit of inhibitory activity was defined as the amount that caused suppression of caseinolytic activity of trypsin or chymotrypsin by one absorbance unit . Estimation of A2M was based on the esterolytic activity of A2M bound chymotrypsin, described in detail by Rao et al (8). Plasma β-hCG levels were measured using the electrochemiluminescence immunoassay method in Elecsys 2010 immunoassay analyzer, using a HCG+β kit from Roche Diagnostics, Germany Statistics : Statistical analysis was done using SPSS version 10.0 using one way ANOVA and values are expressed as Mean ± SD and p values were tabulated.…”

Section: Methodsmentioning

confidence: 99%

Plasma proteinase inhibitory activity in ectopic and molar pregnancy

Roche¹,

Kumar

Ibrahim

et al. 2008

Indian J Clin Biochem

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Antitryptic, antichymotryptic and alpha 2- macroglobulin activities were measured in sera of normal nonpregnant and normal pregnant women and women with tubal ectopic pregnancy and molar pregnancies in the first 5 to 7 weeks of pregnancy calculated from the last menstrual period. While alpha 2-macroglobulin decreased in early normal pregnancy compared to nonpregnant state (p<0.001), in ectopic and molar pregnancies there was an increase in alpha 2- macroglobulin activity (p < 0.001), as compared to nonpregnant and normal pregnant women. Antitryptic activity did not increase in normal and ectopic pregnancy, however was increased in molar pregnancy (p < 0.01). Antichymotryptic activities did not show a change either in normal pregnancy or in cases of ectopic and molar pregnancy. Drop in alpha 2- macroglobulin activity to near normal levels in ectopic, 6 weeks post surgery, correlated well with the decrease in β-hCG. However, in molar pregnancy, alpha 2- macroglobulin remained elevated even when the β-hCG levels in serum returned to zero 10 weeks after surgery. The studies suggest a major role for circulating proteinase inhibitors especially alpha 2-macroglobulin in regulating proteinase activity in normal, ectopic and molar pregnancy.

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“…One unit of inhibitory activity was defined as the amount that caused suppression of caseinolytic activity by one absorbance unit. Estimation of AMG was based on the esterolytic activity of bound alpha chymotrypsin (8).…”

Section: Measurement Of Antiproteinase Activitiesmentioning

confidence: 99%

Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine

Roche

Kusumanjali

Reddy

et al. 2006

Indian J Clin Biochem

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Cyclosporine has been reported to function as an inhibitor of the chymotrypsin like activity of proteasome. We hypothesized that the administration of an exogenous proteinase inhibitor may affect the activities of the Serum antiproteinase activities of serum alpha 2 macroglobulin (AMG), alpha 1-antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) were found to be altered in renal transplant patients receiving cyclosporine. KEY WORDS Alpha-2-macroglobulin,alpha-1-antitrypsin,alpha antichymotrypsin,C2, cyclosporine. Address for Correspondence:Dr.Pragna Rao Kamineni Hospitals Ltd. L.B.Nagar,Hyderabad -500 068 Andhra Pradesh -INDIA E-mail : mnaprag@sancharnet.in We hypothesized that cyclosporine treatment would alter the activities of the naturally occurring anti -proteinases in blood , since cyclosporine would act as an exogenous source of anti -proteinase activity. We selected alpha 2 macroglobulin (AMG), alpha 1-antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) as representatives of the major anti -proteinases in the blood.The effect that cyclosporine has on serum activities of AT, ACT and AMG are presented for the first time in this study. The serum proteinase inhibitory activities were monitored in a few patients who were initially put on cyclosporine therapy immediately after a renal transplant and followed up over a period of 4 weeks as the cyclosporine dose was tapered. The study also evaluated whether the dose of cyclosporine and the measured blood levels of cyclosporine as evidenced by C0 (trough) and C2 (2 hour post dose values) affected serum proteinase inhibitor levels.

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Estimation of serum α2-macroglobulin based on the esterolytic activity of bound α-chymotrypsin

Cited by 6 publications

References 10 publications

Augmented rescue of macroglobulins by supplementation of anti-snake venom with methanolic extract of Andrographis paniculata in Naja naja envenomation

Augmented rescue of macroglobulins by supplementation of anti-snake venom with methanolic extract of Andrographis paniculata in Naja naja envenomation

Plasma proteinase inhibitory activity in ectopic and molar pregnancy

Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine

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