Estimation of synaptic currents by recordings from extracellular electrodes

Chizhov, Anton V.; Pokrovskii, A. N.; Terry, John R.; Sargsyan, Armen

doi:10.1134/s0006350909030166

Cited by 1 publication

(4 citation statements)

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“…In this work, we demonstrate that the zero-space problem defined by Gratiy et al (2011) and the integration constant missing from the calculations of Chizhov et al (2009) are two facets of the same problem: the EC potential pattern by itself does not contain enough information to reconstruct the single-cell IC membrane potential based only on EC measurements. Hamilton et al (2018) have applied an interesting new strategy, called model assimilation, to approximate the IC membrane potential based on the EC measurements.…”

Section: Discussionmentioning

confidence: 99%

“…(2011) and the integration constant missing from the calculations of Chizhov et al. (2009) are two facets of the same problem: the EC potential pattern by itself does not contain enough information to reconstruct the single‐cell IC membrane potential based only on EC measurements.…”

Section: Discussionmentioning

confidence: 99%

“…To overcome the problem and to find a unique solution, the inputs were restricted to be excitatory only. Chizhov et al (2009Chizhov et al ( , 2015 took a different approach. They identified that the 1D traditional CSD is proportional to the second spatial derivative of the EC potential (eqn 3) and it is also proportional to the second spatial derivative of the IC membrane potential, according to the cable equation (eqn 15).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, knowing the spatiotemporal distribution of the membrane potential would allow the distinction between resistive and capacitive membrane components, which is necessary to interpret the observed CSD distributions correctly. To the best of our knowledge, there have been only a few attempts in the literature to cross this obstacle, trying to reconstruct the membrane potential distributions at a population level, but none of them has attempted membrane potential reconstruction at the level of single neurons (Chizhov et al, 2009(Chizhov et al, , 2015. Gratiy et al (2011) calculated the CSD and the subsequent EC potential caused by localized synaptic inputs by using multicompartmental models.…”

Section: Introductionmentioning

confidence: 99%

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Seeing beyond the spikes: reconstructing the complete spatiotemporal membrane potential distribution from paired intra‐ and extracellular recordings

Meszéna

Barlay

Boldog

et al. 2023

The Journal of Physiology

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Although electrophysiologists have been recording intracellular neural activity routinely ever since the ground‐breaking work of Hodgkin and Huxley, and extracellular multichannel electrodes have also been used frequently and extensively, a practical experimental method to track changes in membrane potential along a complete single neuron is still lacking. Instead of obtaining multiple intracellular measurements on the same neuron, we propose an alternative method by combining single‐channel somatic patch‐clamp and multichannel extracellular potential recordings. In this work, we show that it is possible to reconstruct the complete spatiotemporal distribution of the membrane potential of a single neuron with the spatial resolution of an extracellular probe during action potential generation. Moreover, the reconstruction of the membrane potential allows us to distinguish between the two major but previously hidden components of the current source density (CSD) distribution: the resistive and the capacitive currents. This distinction provides a clue to the clear interpretation of the CSD analysis, because the resistive component corresponds to transmembrane ionic currents (all the synaptic, voltage‐sensitive and passive currents), whereas capacitive currents are considered to be the main contributors of counter‐currents. We validate our model‐based reconstruction approach on simulations and demonstrate its application to experimental data obtained in vitro via paired extracellular and intracellular recordings from a single pyramidal cell of the rat hippocampus. In perspective, the estimation of the spatial distribution of resistive membrane currents makes it possible to distiguish between active and passive sinks and sources of the CSD map and the localization of the synaptic input currents, which make the neuron fire. Key points A new computational method is introduced to calculate the unbiased current source density distribution on a single neuron with known morphology. The relationship between extracellular and intracellular electric potential is determined via mathematical formalism, and a new reconstruction method is applied to reveal the full spatiotemporal distribution of the membrane potential and the resistive and capacitive current components. The new reconstruction method was validated on simulations. Simultaneous and colocalized whole‐cell patch‐clamp and multichannel silicon probe recordings were performed from the same pyramidal neuron in the rat hippocampal CA1 region, in vitro. The method was applied in experimental measurements and returned precise and distinctive characteristics of various intracellular phenomena, such as action potential generation, signal back‐propagation and the initial dendritic depolarization preceding the somatic action potential.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%