Estimation of the number of polar molecules in a solvate shell of a fluorescent probe 4-dimethylaminochalcone

Femtosecond-technology-based chemical research

“…Now we will consider two examples of application of two photon femtosecond microscopy in studies of nano scale 42 and biological objects. 43 Excitation of Ag nanoparticles photocatalytically de posited on the surface of TiO 2 nanoparticles to form a mesoporous (Ag/TiO 2 ) film with light of a titanium sapphire laser (λ = 800 nm, pulse duration 100 fs) gave rise to two photon luminescence observed as bright "hot spots". 42 Their luminescence spectra and the dependence of luminescence intensity on the polarization and wave length of incident femtosecond light pulse were studied by scanning femtosecond microscopy.…”

“…43 A living cell was dyed with a membrane fluorescent probe 4 dimethylaminochalcone (DMC), which mainly fluoresces from lipid containing orga nelles. A fluorescent image of a single cell and subcell organelles was obtained for the first time.…”