Estimation of the proportion of cell nuclei in tissue sections falling within tritium-autoradiographic range

Modak, Sohan P.; Lever, W. E.; Therwath, A.M.; Uppuluri, V. R. R.

doi:10.1016/0014-4827(73)90420-5

Cited by 21 publications

(4 citation statements)

References 12 publications

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“…In the rat kidney, a variation in nuclear dimensions exists between diffcrent cell types, intertubular mesenchymal cells possessing nuclei of usually smaller diameters than epithelial cells. Utilizing section thickness of 1-2 pm, however, the error incurred by the disparity in nuclear diameters in relation to the 3H-autoradiographic range was less than 10% (Modak et al, 1973).…”

Section: R E S U L T Smentioning

confidence: 95%

Proliferative Response in the Rat Kidney Induced by a Single, Carcinogenic Dose of Dimethylnitrosamine

Hard

Shaw

1974

Cell Proliferation

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With the application of autoradiography, the pattern of DNA synthetic activity in the rat kidney following a carcinogenic dose of DMN was traced during the 3 weeks following treatment. Depending on cell class and location, DNA synthesis was constantly depressed for the first 1 or 2 days throughout all zones of the kidney. Mesenchymal cells of the cortex and outer band of the outer medulla commenced a wave of stimulated activity at the third day with a peak of response at the sixth day. Epithelial cells of these same two zones were slower to respond, attaining a peak of DNA synthetic activity at the tenth day. By the start of the third week, activity had returned to normal or near‐normal levels. The observations are discussed in relation to the known morphological events which occur in the kidneys of DMN‐treated rats prior to the development of neoplasia.

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Section: R E S U L T Smentioning

confidence: 95%

Proliferative Response in the Rat Kidney Induced by a Single, Carcinogenic Dose of Dimethylnitrosamine

Hard

Shaw

1974

Cell Proliferation

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“…In histological sections of Giemsa-stained non lymphoid tissues, lymphocytes were distinguished by their size, nuclear configuration, and relative lack of cytoplasm. The data obtained in this way from histoautoradiographs were corrected by the method of Modak et al (1973) as described earlier (Pabst and Trepel, 1979). Lymphocytes were considered labeled if more than 5 grains were located over the nucleus.…”

Section: Evaluation Of Autoradiographsmentioning

confidence: 99%

Organ distribution and fate of newly formed splenic lymphocytes in the pig

Pabst

Nowara

1982

Anat. Rec.

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The production of lymphoid cells in the pig spleen was studied autoradiographically after selective labeling of the spleen using an extracorporeal perfusion circuit. Tritiated thymidine was added as a DNA precursor. One to 4 days after local labeling of the spleen the relative and absolute number of spleen-derived lymphocytes were determined in the following organs: mesenteric, cervical and inguinal lymph nodes, thymus, bone marrow, Peyer's patches, tonsils, three different parts of the gut, lung, liver, and blood. The labeled lymphocytes which migrated to these organs were all small lymphocytes, except for some large cells in the lamina propria. In the bone marrow, however, a considerable number of the spleen-derived immigrants were transformed into plasma cells. The total number of labeled lymphocytes decreased dramatically from Day 1 to Day 4 after labeling, indicating a high percentage of short-lived cells. Within the spleen, plasma cells had the highest labeling index of about 30% at Day 1 but this dropped to only 1.5% on Day 3. The organ distribution of the splenic emigrants changed from Day 1 to Day 4 with a relative increase in lymphocytes found in lymph nodes and a decrease in the lung and intestinal wall. The newly formed splenic lymphocytes migrated to T-and B-cell areas in lymph nodes, Peyer's patches and tonsils. In the intestinal wall labeled lymphocytes were found in the lamina propria and also as intraepithelial lymphocytes. There was no obvious redistribution between organ compartments with time after labeling of the spleen. The spleen produces large numbers of lymphocytes, which show typical organ distribution and homing to areas in lymphoid and nonlymphoid organs.

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“…The section thickness (5 /~m) was chosen relative to nuclear size (about 3-4 ~m) in order to minimize the possibility of a radioactive cell appearing in two successive sections (24). Preliminary experiments in which serial sections of selected aggregates were examined indicated that very few radiolabeled cells appeared in two successive sections.…”

Section: Measurements Of Histological Sections and Data Analysismentioning

confidence: 99%

Mobility of normal and virus-transformed cells in cellular aggregates.

Gershman¹,

Drumm²

1975

The Journal of Cell Biology

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The mobility of embryonic chick cells and cells of four established cell lines was examined in cellular aggregates. This was done by preparing aggregates of unlabeled cells and allowing cells of the same type, but prelabeled with [SH]thymidine, to adhere to the surface of the aggregates. After 2-1/2 days in agitated liquid culture the positions of the labeled cells within the aggregates were determined by autoradiographic techniques. Since the labeled and unlabeled cells were otherwise identical, the degree of penetration of the labeled cells into the aggregates was taken as a measure of the mixing or mobility of cells in the aggregate. With this procedure, embryonic chick liver, heart, and neural retina cells were found to move an average of 2.12, 2.68, and 4.00 cell diameters inward, respectively. Mouse fibroblast BALB/c 3T3 cells moved an average of 1.13 cell diameters inward, while Simian virus 40 (SV40)-transformed BALB/c 3T3 cells moved as much as 8.80 cell diameters inward, indicating that cells of the malignant SV40-transformed line were considerably more mobile than the corresponding nonmalignant 3T3 cells. In contrast, cells of the hamster fibroblast line NIL B moved 4.17 cell diameters in 2-1/2 days, while SV40-transformed NIL B cells moved 3.00 cell diameters in the same time. It was therefore concluded that infection with oncogenic viruses does not necessarily result in increased cellular mobility.

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Estimation of the proportion of cell nuclei in tissue sections falling within tritium-autoradiographic range

Cited by 21 publications

References 12 publications

Proliferative Response in the Rat Kidney Induced by a Single, Carcinogenic Dose of Dimethylnitrosamine

Proliferative Response in the Rat Kidney Induced by a Single, Carcinogenic Dose of Dimethylnitrosamine

Organ distribution and fate of newly formed splenic lymphocytes in the pig

Mobility of normal and virus-transformed cells in cellular aggregates.

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