Objective: This study aimed to measure different segmental measurements of dry femur bone and to use them to derive the regression equation to estimate the total length of femur bones. Materials and Methods: Study was conducted among 60 dry femora disarticulated from the donated cadavers and unclaimed body of Nepalese population. Three longitudinal (segment 1-3) and one circumferential measurement mid-shaft circumference (MSC) were measured along with the maximum femoral length using osteometric board and measuring plastic tape. All measurements were recorded by the same observer twice, and average of two measurements was recorded as a final measurement for recording. Results: Mean length of femur, MSC, segments 1-3 was 41.93 ± 2.47 cm, 8.31 ± 0.59 cm, 15.85 ± 1.57 cm, 10.71 ± 1.76 cm, and 15.37 ± 1.22 cm, respectively. Except for segment 1, all other segmental measurements were slightly higher in right sided femora; but the difference was statistically not significant. All the segmental measurements were found to be positively correlated with the femoral length. The value of coefficient was highest for segment 1 (0.660) followed by MSC (0.625), segment 3 (0.624), and segment 2 (0.379). Conclusion: Femoral length could be estimated with the help of segmental measurements of it using regression equation method among Nepalese group of the population.