Estimation of uronic acids using diverse approaches and monosaccharide composition of alkali soluble polysaccharide from Vitex negundo Linn.

Kumar, Pradeep; Kumar, Vineet

doi:10.1016/j.carbpol.2017.02.034

Cited by 49 publications

(9 citation statements)

References 33 publications

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“…For the determination of pectin content, galacturonic acid was measured using the modified carbazole method and the pectin content was determined based on galacturonic acid 35 . Sulfuric acid (6 mL) was placed in an ice bath and 0.5 mg mL −1 pectin solution was added.…”

Section: Methodsmentioning

confidence: 99%

Effect of ultrasonic pretreatment monitored by real‐time online technologies on dried preparation time and yield during extraction process of okra pectin

Zhang

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND Ultrasonic pretreatment is a novel physical method that can be used in the extraction process of okra pectin. Real‐time online monitoring technologies were introduced in time and frequency domains when okra was pretreated. Preparation time of dried okra and yield of okra pectin were studied; and physicochemical properties of okra pectin were analyzed at the optimum ultrasonic parameter. RESULTS Results showed that ultrasonic intensity of sweeping‐frequency ultrasonic (SFU) pretreatment was stronger than that of fixed‐frequency ultrasonic pretreatment (FFU). SFU pretreatment (60 ± 1 kHz) at 30 min had a strong ultrasonic voltage peak of 0.05387 V and signal power peak of −6.62 dBm. The preparation time of dried okra was 160 ± 14.14 min in the pretreated group, 44.83% lower than control without SFU pretreatment. The intercellular space was 56.03% higher than control. Water diffusion coefficient increased from 1.41 × 10−9 to 2.14 × 10−9 m2 s−1. Monobasic quadratic equations were developed for the monitored ultrasonic intensity and pectin yield. Compared to control, extraction yield (16.70%), pectin content (0.564 mg mg−1), solubility (0.8187 g g−1) and gel strength (30.91 g) were improved in the pretreated group. Viscosity decreased, and values of G′ and G″ crossing at 63 rad s−1 revealed the viscoelastic behavior and the beginning of viscous behavior with a sol state. CONCLUSION Decrement of dried preparation time and increment of yield were achieved by ultrasonic pretreatment during the extraction process of okra pectin, and the relationship of ultrasonic intensity monitored by real‐time online technologies and yield was given. © 2021 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Effect of ultrasonic pretreatment monitored by real‐time online technologies on dried preparation time and yield during extraction process of okra pectin

Zhang

Wang

et al. 2021

J Sci Food Agric

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“…The content of sulfate radical was assessed using the reported way [32]. The uronic acid content of PCPP and PCPP-1a was measured by m-hydroxydiphenyl method using D-galacturonic acid as a standard [33]. The protein content was measured conforming to the Bradford method using bovine serum albumin (BSA) as a standard [34].…”

Section: Methodsmentioning

confidence: 99%

Enzymatic Extraction, Purification, and Characterization of Polysaccharides fromPenthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

Lin

Zhao

Deng

et al. 2018

BioMed Research International

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Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

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“…14 The uronic acid content was determined by the carbazole-sulfuric acid method with galacturonic acid as the standard. 15 The soluble protein was measured according to the Coomassie brilliant blue G-250 method with Bovine serum albumin (BSA) as the standard. 16 The monosaccharide composition of the samples was analyzed by gas chromatography (Techcomp GC-7900, Shanghai, China) according to the method described in our previous study.…”

Section: Chemical Composition and Monosaccharide Composition Of Csp A...mentioning

confidence: 99%

Spheroidization of ultrasonic degraded corn silk polysaccharide to enhance bioactivity by the anti‐solvent precipitation method

Jia

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND: Corn silk is a very important by-product of corn production with medicinal value. Corn silk polysaccharide (CSP) is the main active ingredient. In the present study, ultrasound and spheroidization by anti-solvent were applied to improve the biological activity of CSP. RESULTS:The results showed that ultrasonic degradation improved the ⊍-glucosidase inhibitory activity of CSP by changing its physicochemical characteristics. As the anti-solvent ratio increased, the particle size of the nanoparticles (NPs) from the spheroidization of ultrasonic-degraded corn silk polysaccharide (UCSP) gradually increased, and NP-1 exhibited the highest inhibitory effect of ⊍-glucosidase. Isothermal titration calorimetry (ITC) results indicated that the enhanced activity might be due to more ⊍-glucosidase binding sites with NP-1 compared with no spheroidization. Western blotting results showed that NP-1 could improve the 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG) uptake in the L6 cells by regulating the phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway and the translocation of glucose transporter 4 (GLUT4). NP-1 also exhibited excellent stability in different environments.CONCLUSION: The study revealed that ultrasonic treatment and spheroidization processing showed potential applications for improving the biological activity of polysaccharides.

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Estimation of uronic acids using diverse approaches and monosaccharide composition of alkali soluble polysaccharide from Vitex negundo Linn.

Cited by 49 publications

References 33 publications

Effect of ultrasonic pretreatment monitored by real‐time online technologies on dried preparation time and yield during extraction process of okra pectin

Effect of ultrasonic pretreatment monitored by real‐time online technologies on dried preparation time and yield during extraction process of okra pectin

Enzymatic Extraction, Purification, and Characterization of Polysaccharides fromPenthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

Spheroidization of ultrasonic degraded corn silk polysaccharide to enhance bioactivity by the anti‐solvent precipitation method

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