Estimation of Vitamin D Gingival Crevicular Fluid Level In Patients With Different Periodontal Conditions.

Abstract

Background: Vitamin D (Vit. D) is responsible in the process of calcium homeostasis, among many other biological effects. Thus it plays an essential role in bone related diseases. Furthermore, several studies investigated the link between Vit. D plasma level and various conditions of periodontal diseases.Objective: Therefore, the present study aimed to assess Vit. D3 [25(OH) D] gingival crevicular fluid (GCF) levels in individuals with different periodontal conditions.Subjects and methods: 60 GCF samples were taken from 60 individuals divided into 3 equal groups, healthy, gingivitis and periodontitis. Vit. D GCF level was assessed using ELISA technique.Results: There was a statistically significant difference between (gingivitis, periodontitis and healthy control) groups, with mean and SD [4.4 + 0.3 ng/ml, 3.5 + 0.4 ng/ml, 5.6 + 0.5 ng/ml] respectively (P value 0.001). Conclusion:The results are supporting the link between 25(OH) D GCF level and the periodontal condition. The study might be emphasizing the correlation between the periodontal disease and deficiency in Vit. D.

Periodontal disease and visfatin level: A systematic review and meta-analysis

“…Banna SE et al also confirmed the relationship between the severity and progression of periodontal disease with visfatin levels. In their study, visfatin level was higher in chronic periodontitis and gingivitis than in healthy subjects, but the difference between the chronic periodontitis group and gingivitis group was not statistically significant [25] as we did. Turer CC et al [43] also supposed that the level of visfatin in patients with gingivitis was higher than in healthy patients, and chronic periodontitis was higher than in both cases and in fact, the concentration of visfatin was proportional to the severity of the inflammation of the disease.…”

“…Only in two studies [ 16 , 25 ], visfatin levels in GCF were compared between gingivitis patients and healthy individuals, and 20 healthy cases and 30 gingivitis patients were included in the analysis, and SMD was estimated as 2.60 ng/ml (95% confidence interval (CI): -0.40–5.62, p = 0.090) ( Table 2 ). The results of the present study showed that the visfatin level in the GCF of gingivitis patients and healthy individuals are the same.…”

“…In nine studies [ 16 , 17 , 24 , 25 , 28 , 30 , 32 , 33 , 36 ], visfatin level of GCF was compared between chronic periodontitis patients and healthy individuals, which includes 152 healthy cases and 172 chronic periodontitis patients. The results of the meta-analysis revealed that SMD = 4.32 ng/ml (95% CI: 2.71–5.93, P<0.001) ( Fig 2 , Table 2 ) so Visfatin levels in GCF were significantly higher in periodontitis in comparison to the control group.…”

“…Only in two studies [ 16 , 25 ], visfatin levels in GCF were compared between gingivitis patients and chronic periodontitis patients, and 30 gingivitis patients and 30 chronic periodontitis patients were included in the analysis, SMD was estimated as 3.59 ng/ml (95%CI: -2.11–9.30, p = 0.217) ( Table 2 ). The results of the present study showed no significant visfatin level differences in the GCF of gingivitis patients and chronic periodontitis patients.…”

“…After screening the title and abstract of these studies by two authors independently, 58 studies that did not meet the inclusion criteria were excluded and the full text of the remaining 33 studies was examined. Finally, sixteen studies [16,17,19,[24][25][26][27][28][29][30][31][32][33][34][35][36] were included in the meta-analysis. Seventeen studies that were impossible to include in the meta-analysis are presented in S2 Table with details and reasons [14,15,18,[37][38][39][40][41][42][43][44][45][46][47][48][49][50] and can be considered as qualitative data.…”