Estimation of Water-Soluble Vitamin B-Complex in Selected Leafy and Non-Leafy Vegetables by HPLC Method

Mozumder, N. H. M. Rubel; Akhter, Most. Jesmin; Khatun, Anwara Akter; Rokibuzzaman, Mohammad; Akhtaruzzaman, Mohammad

doi:10.13005/ojc/350414

Cited by 15 publications

(11 citation statements)

References 14 publications

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“…Adequacy of HPLC-DAD method [12] which was used in this study for determining vitamins B1, B2, B3 and B6 was confirmed in a validation process through satisfactory results for limits of quantification, linearity, reproducibility and accuracy as well as through interlaboratory comparison testing. Results established through dietary supplement analysis were interpreted based on allowed tolerance as defined in the Guidance document [13] [14], као и електрохемијски детектор [15]. У овом раду од интереса су били радови где је примењен HPLC систем са DAD детектором.…”

Section: Discussionmentioning

confidence: 99%

“…From the scientific literature data, it can be inferred that the fundamental technique for determination of vitamins B1, B2, B3 and B6 is precisely the HPLC technique coupled with a variety of detectors. In addition to DAD detector, which was used in this study, for determining B-group vitamins PDA detector [14] as well as electrochemical detector [15] are used. In this research, the studies where HPLC system was coupled with DAD detector were of interest.…”

Section: Discussionmentioning

confidence: 99%

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Determination of content of vitamins B1, B2, B3 and B6 in dietary supplements

Dodevska,

Milenković,

Nedeljković

et al. 2022

Glas javnog zdr

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Uvod "Dodaci ishrani (dijetetski suplementi) su hrana koja dopunjuje uobičajenu ishranu, i koja predstavlja kon-centrovane izvore hranljivih sastojaka ili drugih sastojaka sa hranljivim ili fiziološkim efektom, pojedinačno ili u kombinaciji, a u prometu se nala-ze u doziranim oblicima kao što su kapsule, pasti-le, tablete i slično, kesice praška, ampule tečnosti, bočice sa kapaljkom, i ostalim sličnim oblicima Abstract Use of dietary supplements is very widespread and constantly on the rise. B-group vitamins are among the most frequently used dietary supplements since factors like age, pregnancy, impaired thyroid function, anorexia, genetic factors, use of medication and alcohol increase the needs for B-group vitamins. Given that the above mentioned conditions require supplementation it is necessary that dietary supplements contain the quantities specified on the labeling. It is thus necessary to test their quality i.e., the quantities of active ingredients belonging to B-group vita-mins in dietary supplements to determine if the obtained values comply with the declared ones. Vitamins B1, B2, B3 and B6 are important components of the B-complex group. In this study the content of vitamins B1, B2, B3 and B6 was determined in the samples of 29 different dietary supplements present on the market of the Republic of Serbia, using HPLC-DAD method with gradient elution. Obtained results have shown that, of all tested samples, 86.2% comply with the declared values for all four B-group vitamins. Values for vitamin B3 were in accordance with the declared values in all tested supplements. Of other analysed vitamins, B1 fulfilled the declared requirements in 89.7% of samples, while the quantity of active substance of vitamins B2 and B6 was within declared limits in 93.1% of tested dietary supplements. It is necessary to expand this type of studies on a larger number of samples, since controlling the declaration i.e., determining the quantity of specific active components present in supplements indirectly impacts prevention in the population subgroups in need of supplementation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Determination of content of vitamins B1, B2, B3 and B6 in dietary supplements

Dodevska,

Milenković,

Nedeljković

et al. 2022

Glas javnog zdr

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“…The mobile phase consisted of a mixture of 100-mM sodium phosphate buffer (pH 2.2) containing 0.8-mM sodium-1-octane sulfonate and acetonitrile at a 9:1 (v/v) ratio at 40 • C. The flow rate was kept constant at 0.8 mL min −1 , and a PDA detector was used with an absorption rate of 270 nm. The detection and quantification of B vitamins was determined according to the report published by Mozumder et al [49].…”

Section: Vitamin Analysis In Raisinsmentioning

confidence: 99%

Drying Grapes after the Application of Different Dipping Solutions: Effects on Hormones, Minerals, Vitamins, and Antioxidant Enzymes in Gök Üzüm (Vitis vinifera L.) Raisins

Keski̇n

Kaya

Ateş³

et al. 2022

Plants

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(1) Background: Raisins contain a wide range of hormones, minerals, vitamins, and antioxidant enzymes that may contribute to the health benefits of consumers. (2) Methods: The aim of this research was to compare the hormone, mineral, vitamin, and antioxidant capacities of Gök Üzüm (Vitis vinifera L.) raisins immersed in oak ash (OA) and potassium carbonate (PC) dipping solutions before drying. (3) Results: Abscisic acid (ABA) (5751.18–11,868.40 ng g−1) and riboflavin (95.17–135.54 mg 100 g−1) were the most abundant hormone and vitamin quantified in Gök Üzüm raisins. Glutathione S-transferase (540.07–744.85 EU gr berry−1), 6-glucose phosphate dehydrogenase (214.50–317.43 EU gr berry−1), and glucose-6-phosphate dehydrogenase (208.25–241.86 EU gr berry−1) enzymes presented the highest antioxidant activity in the samples. Raisins obtained after drying by immersion in OA presented higher indol-3-acetic acid (IAA), ABA, salicylic acid (SA), cytokinins (CK), and zeatin contents; glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), glutathione S-transferase (GST), 6 glucose phosphate dehydrogenase (6GPD), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) enzymatic activity; vitamin B1, B2, B6, C, and A contents; and nitrogen (N), phosphorus (P), manganese (Mn), calcium (Ca), sulfur (S), potassium (K), iron (Fe), sodium (Na), and magnesium (Mg) levels compared to the grapes dried after PC applications. (4) Conclusions: Drying Gök Üzüm grapes after the application of OA dipping solution promotes a higher content of hormones, minerals, vitamins, and antioxidant enzymes compared to PC treatments. These results could help raisin producers to make decisions when using a dipping solution to dry grapes.

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“…Water soluble vitamin B1, B2 and C were estimated following HPLC method with little bit modification [17].…”

Section: Vitamins (B1 B2 and C) Analysismentioning

confidence: 99%

Phytochemicals and Nutritional Constituent Evaluation of Bael (Aegle marmelos) Fruit Pulp at Different Development Stage

Sarkar

Rashid

Musarrat

et al. 2021

AFSJ

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Aegle marmelos Correa commonly known as “Bael,” has been recognized as a component of traditional medication for the treatment of various human ailments. The present study was focused on phytochemical screening, nutritional constituent of A. marmelos at different development stages. Highest amount of alkaloid was in premature bael (8.09±0.09 mg/g), phenols in premature bael (9.65±0.06 mg/g) pulp and saponins in mature bael(5.57±0.08) pulp. Highest amount of thiamin (B1) (1.83±0.03 mg/100 g) and ascorbic acid (48.62±0.04 mg/100 g) in premature bael pulp. Sugar content significantly highest in matured bael(6.94±0.04 mg/100 g) pulp. Most abundant mineral potassium content was maximum in (139.61±0.04 mg/100 g) premature bael fruit pulp. The nutritional constituents and phytochemicals change depending on maturation stage. Nutritional constituent changes on the effects of development of bael (Aegle marmelos) fruit. It has been found in the present study that there were a numbers of phytochemical changes occurred during different fruit development stages.

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Estimation of Water-Soluble Vitamin B-Complex in Selected Leafy and Non-Leafy Vegetables by HPLC Method

Cited by 15 publications

References 14 publications

Determination of content of vitamins B1, B2, B3 and B6 in dietary supplements

Determination of content of vitamins B1, B2, B3 and B6 in dietary supplements

Drying Grapes after the Application of Different Dipping Solutions: Effects on Hormones, Minerals, Vitamins, and Antioxidant Enzymes in Gök Üzüm (Vitis vinifera L.) Raisins

Phytochemicals and Nutritional Constituent Evaluation of Bael (Aegle marmelos) Fruit Pulp at Different Development Stage

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