Estradiol-17β and testosterone concentrations in male and female Mya arenaria (Mollusca bivalvia) during the reproductive cycle

Gauthier-Clerc, Sophie; Pellerin, J.; Amiard, J. C.

doi:10.1016/j.ygcen.2005.08.004

Cited by 114 publications

(56 citation statements)

References 32 publications

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“…E 2 concentrations were close to concentrations in the soft tissue of M. edulis (ReisHenriques et al 1990), but lower than the ones in My. arenaria (Gauthier-Clerc et al 2006), P. yessosensis (Osada et al 2004), and M. edulis . T concentrations were higher than E 2 concentrations, and their range appeared to be similar to that of My.…”

Section: Discussionmentioning

confidence: 99%

“…1E). E 2 and T concentrations were estimated using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Cayman Chemical Company, United States) as described by Gauthier-Clerc et al (2006). Samples (0.3 g) of gonad tissue (from 5 individuals every month) were individually homogenised in H 2 O (1:5 w:w) and sonicated twice for 30 s. 1200 μl of 25 mM HCl were added to 1500 μl homogenate and allowed to stand for 15 min at 40°C.…”

Section: Methodsmentioning

confidence: 99%

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Estradiol‐17β and testosterone levels in the cockleFulvia muticaduring the annual reproductive cycle

Liu

Li²,

Kong

2008

New Zealand Journal of Marine and Freshwater Research

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In this study, we investigated seasonal variations in the concentrations of estradiol-17β (E 2 ) and testosterone (T) in the gonad of the cockle Fulvia mutica from March 2004 to February 2005 in relation to the reproductive cycle. Histological data showed that F. mutica is a hermaphrodite bivalve species, and gametogenesis took place during winterspring (November-April) and spawning occurred in summer (May-June) when water temperature was higher. E 2 and T concentrations were measured by enzyme-linked immunosorbent assay (ELISA). E 2 concentrations varied between 7.27 and 32.63 pg/g wet weight and T concentrations ranged from 22.83 to 77.15 pg/g wet weight throughout the year. Both steroids exhibited a similar annual pattern that correlated with the reproductive cycle. The two peaks of E 2 and T concentrations in November and MayJune occurred at critical moments of the F mutica reproductive cycle: the onsets of gametogenesis and of the spawning period. E 2 andT concentrations were low during the non-reproductive period extending from July to October. These results suggest that E 2 and T could play a role as endogenous modulators in the reproductive cycle of F. mutica.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Estradiol‐17β and testosterone levels in the cockleFulvia muticaduring the annual reproductive cycle

Liu

Li²,

Kong

2008

New Zealand Journal of Marine and Freshwater Research

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“…Steroid concentrations reported in the literature in bivalve tissues widely differed (from picograms to nanograms) depending on the species, tissue, sexual maturation stage, and analytic technique (21). The only available data on estrogen content in the hemolymph are those reported by Stefano et al (46) and Zhu et al (55) in Mytilus edulis.…”

Section: Discussionmentioning

confidence: 99%

“…17␤-Estradiol (E 2 ) can modulate the function of neutrophil granulocytes, monocytes, and macrophages; these cell types have been reported to express intracellular and membrane estrogen receptors (ERs), and their response to E 2 can be mediated by nuclear, classical or "genomic" pathways, as well as by rapid, "nongenomic" mechanisms of action (2,3,24,45). These latter mechanisms may be initiated at membrane or cytosolic locations (29,39,49) and can result in direct local effects (e.g., modification or ion fluxes) and regulation of gene transcription secondary to activation of cytosolic kinase cascades (5,22,24,29,43).Among invertebrates, estrogens have been identified in bivalve molluscs (25,34,40,43), in which their role has been mainly investigated in the control of gametogenesis (20,21,30,33,34). However, there is evidence that estrogen can represent an important signaling molecule that is involved in roles other than reproduction in these organisms.…”

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Immunomodulation by 17β-estradiol in bivalve hemocytes

Canesi

Ciacci²,

Lorusso³

et al. 2006

American Journal of Physiology-Regulatory, Integrative and Comp

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.-In mammals, estrogens have dose-and cell-type-specific effects on immune cells and may act as pro-and anti-inflammatory stimuli, depending on the setting. In the bivalve mollusc Mytilus, the natural estrogen 17␤-estradiol (E2) has been shown to affect neuroimmune functions. We have investigated the immunomodulatory role of E 2 in Mytilus hemocytes, the cells responsible for the innate immune response. E2 at 5-25 nM rapidly stimulated phagocytosis and oxyradical production in vitro; higher concentrations of E 2 inhibited phagocytosis. E2-induced oxidative burst was prevented by the nitric oxide (NO) synthase inhibitor N G -monomethyl-L-arginine and superoxide dismutase, indicating involvement of NO and O 2 Ϫ ; NO production was confirmed by nitrite accumulation. The effects of E2 were prevented by the antiestrogen tamoxifen and by specific kinase inhibitors, indicating a receptor-mediated mechanism and involvement of p38 MAPK and PKC. E2 induced rapid and transient increases in the phosphorylation state of PKC, as well as of a aCREB-like (cAMP responsive element binding protein) transcription factor, as indicated by Western blot analysis with specific anti-phospho-antibodies. Localization of estrogen receptor-␣-and -␤-like proteins in hemocytes was investigated by immunofluorescence confocal microscopy. The effects of E2 on immune function were also investigated in vivo at 6 and 24 h in hemocytes of E2-injected mussels. E2 significantly affected hemocyte lysosomal membrane stability, phagocytosis, and extracellular release of hydrolytic enzymes: lower concentrations of E2 resulted in immunostimulation, and higher concentrations were inhibitory. Our data indicate that the physiological role of E2 in immunomodulation is conserved from invertebrates to mammals. estrogen; innate immunity; kinase-mediated cell signaling; Mytilus IN MAMMALS, ESTROGENS EXERT a broad spectrum of activities on a wide variety of cells and tissues, including the immune system. Estrogens have dose-and cell-type-specific effects on immune cells and may act as pro-and anti-inflammatory stimuli, depending on the setting (5,22,24,43). 17␤-Estradiol (E 2 ) can modulate the function of neutrophil granulocytes, monocytes, and macrophages; these cell types have been reported to express intracellular and membrane estrogen receptors (ERs), and their response to E 2 can be mediated by nuclear, classical or "genomic" pathways, as well as by rapid, "nongenomic" mechanisms of action (2,3,24,45). These latter mechanisms may be initiated at membrane or cytosolic locations (29,39,49) and can result in direct local effects (e.g., modification or ion fluxes) and regulation of gene transcription secondary to activation of cytosolic kinase cascades (5,22,24,29,43).Among invertebrates, estrogens have been identified in bivalve molluscs (25,34,40,43), in which their role has been mainly investigated in the control of gametogenesis (20,21,30,33,34). However, there is evidence that estrogen can represent an important signaling molecule that is involved in roles ot...

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Testosterone levels and fecundity in the hermaphroditic aquatic snail Lymnaea stagnalis exposed to testosterone and endocrine disruptors

Giusti

Ducrot

Joaquim-Justo

et al. 2013

Enviro Toxic and Chemistry

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Endocrine disruptors are known to alter endogenous free and esterified levels of androgenic and estrogenic steroid hormones in aquatic mollusks. The origin of steroids in these animals, however, remains controversial. In the present study, free and esterified testosterone concentrations were measured in the hermaphroditic aquatic gastropod Lymnaea stagnalis exposed to molecules known for their androgenic (testosterone and tributyltin), anti-androgenic (cyproterone-acetate), and estrogenic (chlordecone) properties, by reference to their mode of action in vertebrates. In parallel, snail oviposition and fecundity were followed over a 21-d exposure period. Testosterone exposure resulted in increased esterified testosterone levels, whereas free testosterone concentrations remained stable. In contrast, cyproterone-acetate significantly increased the free form of testosterone with no changes in the esterified form, whereas chlordecone showed a tendency to reduce (though not significantly) esterified testosterone concentrations without changing free testosterone levels. Finally, tributyltin did not alter testosterone homeostasis. The production of egg clutches and eggs was significantly reduced only in the snails exposed to the highest concentrations of chlordecone (19.6 µg/L) and tributyltin (94.2 ng Sn/L). Overall, the present study demonstrates that uptake of testosterone from the exposure medium occurs in L. stagnalis. Moreover, it shows that cyproterone-acetate and, to a lesser extent, chlordecone can alter endogenous testosterone levels in this freshwater snail. However, the relationship between hormonal changes and snail reproduction has not been established. Environ Toxicol Chem 2013;32:1740-1745. © 2013 SETAC.

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Estradiol-17β and testosterone concentrations in male and female Mya arenaria (Mollusca bivalvia) during the reproductive cycle

Cited by 114 publications

References 32 publications

Estradiol‐17β and testosterone levels in the cockleFulvia muticaduring the annual reproductive cycle

Estradiol‐17β and testosterone levels in the cockleFulvia muticaduring the annual reproductive cycle

Immunomodulation by 17β-estradiol in bivalve hemocytes

Testosterone levels and fecundity in the hermaphroditic aquatic snail Lymnaea stagnalis exposed to testosterone and endocrine disruptors

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