Estradiol-17β Stimulates the Renewal of Spermatogonial Stem Cells in Males

Miura, Takeshi; Miura, Chiemi; Ohta, Toshio; Nader, Manal; Todo, Takashi; Yamauchi, Kazuyo

doi:10.1006/bbrc.1999.1494

Cited by 247 publications

(143 citation statements)

References 17 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…Moreover, in eel testis ex vivo, the stimulatory effect decreased with increasing (0 . 01-1 ng/ ml) oestrogen doses (Miura et al 1999), the concentration used in the present study being approximately three times higher than the maximum concentration used for eel testis.…”

Section: Discussionmentioning

confidence: 59%

“…All types of ER are expressed in testis (Hawkins et al 2000, Menuet et al 2002, Choi & Habibi 2003, Halm et al 2004, Filby & Tyler 2005. Studies describing their cellular localization in testis indicate a heterogeneous pattern of mRNA and protein expression, for example, Esr1 protein has been found in the interstitial tissue of rainbow trout (Oncorhynchus mykiss) testis (Bouma & Nagler 2001), esr1 and esr2 mRNA expressions were found in Japanese common goby (Acanthogobius flavimanus) and Japanese eel (Anguilla japonica) Sertoli cells (Miura et al 1999, Ito et al 2007, while Esr1 and Esr2 proteins were produced by meiotic and postmeiotic germ cells in channel catfish (Ictalurus punctatus) testis (Wu et al 2001). Functionally, oestrogens stimulate spermatogonial stem cell renewal in immature Japanese eel testis probably via Sertoli cells (Miura et al 1999(Miura et al , 2003.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Waal¹,

Leal²,

García-López³

et al. 2009

Journal of Endocrinology

View full text Add to dashboard Cite

Androgens can induce complete spermatogenesis in immature or prepubertal teleost fish. However, many aspects of the role of androgens in adult teleost spermatogenesis have remained elusive. Since oestrogens inhibit androgen synthesis, we used an oestrogen-induced androgen depletion model to identify androgen-dependent stages during adult zebrafish spermatogenesis. Exposure to 10 nM 17b-oestradiol (E 2 ) in vivo at least halved the mass of differentiating germ cells (from type B spermatogonia to spermatids), while type A spermatogonia accumulated. Studies on the cellular dynamics revealed that a reduction of spermatogonial proliferation together with an inhibition of their differentiation to type B spermatogonia were the basis for the oestrogen-mediated disturbance of spermatogenesis. The capacity of the zebrafish testis to produce 11-ketotestosterone as well as the expression of steroidogenesis-related genes was markedly decreased after in vivo oestrogen exposure. Moreover, the androgen-release response to recombinant zebrafish Lh was lost after oestrogen exposure. We conclude that oestrogen exposure caused a state of androgen insufficiency in adult male zebrafish. Since the downregulation of the steroidogenic system as well as the disturbance of spermatogenesis in testicular explants exposed to E 2 ex vivo was much less severe than after in vivo exposure, the main inhibitory effect appears to be exerted via feedback inhibition of gonadotropin release. This experimental set-up helped to identify spermatogonial proliferation and their differentiation as androgen targets in adult zebrafish spermatogenesis.

show abstract

Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Waal¹,

Leal²,

García-López³

et al. 2009

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Surprisingly, E2 levels were significantly higher in the mixedsex groups than in all-females groups during the entire course of the study. Although in some species males could display high levels of estradiol [39], this result is difficult to explain. Nevertheless, the general increase of T, 11KT and E2 levels observed after the onset of sexual differentiation (D35-D70) in both progenies suggest an increase of the steroidogenesis and steroid activity after the onset of gonadal differentiation, as it was observed in Nile tilapia and Coho salmon [8,13].…”

Section: Discussionmentioning

confidence: 98%

Sex steroid dynamics during embryogenesis and sexual differentiation in Eurasian perch, Perca fluviatilis

et al. 2007

View full text Add to dashboard Cite

It is widely accepted that sex steroid hormones play an important and a specific role during the process of sex differentiation in fish. In order to describe the role of the three main sex steroid hormones (testosterone-T, 17b-estradiol-E2 and 11keto-testosterone-11KT) during embryogenesis and sex differentiation in Eurasian perch, Perca fluviatilis, eggs, larvae and juveniles originating from two mixed-sex and two all-female progenies were regularly sampled from fertilization to hatching (D0) and from hatching to day 70 post-hatching (D70). Just after spawning, a significant amount of sex steroids [T (1634.2 pg g ) and significantly increased after the onset of the histological differentiation of the gonad in both progenies (D35). Levels of 11KT were significantly higher in mixed-sex (median: 431.5 pg g À1) than in all-female progenies (below the limit of assay detection) and significantly increased at D35 in all-female progenies (median value: 343.2 pg g À1 ). Mean 11KT to E2 ratio was six-fold higher in mixed-sex progenies (1.35) than in all-female progenies (0.24). The data suggest that the 11-oxygenated androgen (11KT) plays a major role in the male differentiation process, and that sex differentiation in Eurasian perch is probably determined by the 11KT to E2 ratio. #

show abstract

“…E2 appears to be required for mitotic proliferation of germ cells in the Japanese huchen (Hucho perryi) (Amer et al 2001) and in the Japanese eel (Anguilla japonica). Furthermore, spermatogonial divisions were stimulated when testis organ cultures were exposed for 15 days to 10, 100 and 1000 ng/L E2 (Miura et al 1999). In rat gonocytes (or prespermatogonial cells), a stimulating effect of estrogen on gonocyte proliferation was reported (Li et al al.…”

Section: Discussionmentioning

confidence: 99%

Effect of 17‐α‐ethynylestradiol on germ cell proliferation in organ and primary culture of medaka (Oryzias latipes) testis

Song

Gutzeit

2003

Dev Growth Differ

View full text Add to dashboard Cite

Organ cultures and primary cell cultures of medaka ( Oryzias latipes ) testis were compared with respect to cell viability and cell proliferation. The analysis by fluorescence microscopy and flow cytometry showed that in both cultures the cells remained viable for at least 1 day and cell proliferation could be analyzed reliably by BrdU incorporation. The proliferating cells were mostly spermatogonia located at the periphery of the testis in tissue sections. Both culture systems were used to study the effect of 17-␣ -ethynylestradiol on cell proliferation. The results obtained with organ and primary cultures were consistent: low concentrations (0.01 and 1 n M ) of synthetic estrogen stimulated cell proliferation slightly, while a higher concentration (100 n M ) had an inhibitory effect. Both culture methods are suitable for the analysis of substances that might interfere with germ cell proliferation or other functions in spermatogenesis.

show abstract

Estradiol-17β Stimulates the Renewal of Spermatogonial Stem Cells in Males

Cited by 247 publications

References 17 publications

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis

Sex steroid dynamics during embryogenesis and sexual differentiation in Eurasian perch, Perca fluviatilis

Effect of 17‐α‐ethynylestradiol on germ cell proliferation in organ and primary culture of medaka (Oryzias latipes) testis

Contact Info

Product

Resources

About