Estradiol Down‐Regulates LPS‐Induced Cytokine Production and NFkB Activation in Murine Macrophages

Deshpande, Rohini; Khalili, Houman; Pergolizzi, Robert G.; Michael, Sandra D.; Chang, Mei

doi:10.1111/j.1600-0897.1997.tb00275.x

Cited by 270 publications

(158 citation statements)

References 52 publications

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“…As mentioned above, exposure of macrophages in vitro to estrogen causes an increase in production of TNF-α and IL-1β [116,117]. Expression of other cytokines, like IL-6, are more dose-dependent in that proestrus levels of estrogen inhibit IL-6, whereas pregnancy levels of estrogen increases expression of IL-6 [30,118,119]. Cytokine regulation, by steroid hormones, including estrogen, occurs in two major fashions based on the presence of estrogen response elements (ERE) in the promoter region of the gene.…”

Section: Mechanisms Sectionmentioning

confidence: 96%

“…This regulation by estrogen is demonstrated by the dramatic increase in expression of IFN-γ mRNA following stimulation of cells with estrogen [120]. All other cytokines, such as IL-6, lack an ERE [121,122], but can be tightly regulated by estrogen [119]. IL-6 expression is regulated indirectly through the binding of estrogen to its receptor.…”

Section: Mechanisms Sectionmentioning

confidence: 99%

“…The receptor homo-or hetero-dimers bind to fos or jun thereby blocking their ability to bind to AP-1 sites. Additionally, the receptor dimmers can bind to NF-κB or CEBP-β [119,123]. NF-κB has been observed to be up-regulated in patients with SIRS along with the cytokines that it is known to regulate, indicating that NF-κB may be an important mediator for estrogen's effect on the immune response after injury [124].…”

Section: Mechanisms Sectionmentioning

confidence: 99%

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Sex differences and estrogen modulation of the cellular immune response after injury

Bird¹,

Karavitis²,

Kovacs³

2008

Cellular Immunology

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Cell-mediated immunity is extremely important for resolution of infection and for proper healing from injury. However, the cellular immune response is dysregulated following injuries such as burn and hemorrhage. Sex hormones are known to regulate immunity, and a well-documented dichotomy exists in the immune response to injury between the sexes. This disparity is caused by differences in immune cell activation, infiltration, and cytokine production during and after injury. Estrogen and testosterone can positively or negatively regulate the cellular immune response either by aiding in resolution or by compounding the morbidity and mortality. It is apparent that the hormonal dysregulation is dependent not only on the type of injury sustained but also the amount of circulating hormones. Therefore, it may be possible to design sex-specific therapies to improve immunological function and patient outcome.

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Section: Mechanisms Sectionmentioning

confidence: 96%

Section: Mechanisms Sectionmentioning

confidence: 99%

Section: Mechanisms Sectionmentioning

confidence: 99%

See 1 more Smart Citation

Sex differences and estrogen modulation of the cellular immune response after injury

Bird¹,

Karavitis²,

Kovacs³

2008

Cellular Immunology

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“…26 The potential effect of estrogen in resolving HC remains unclear, but there is some evidence that estrogen has a profound effect on the cellular and cytokine immune response during inflammation. 27 Some authors reported that estradiol enhances the healing of tissue damaged by trauma. 28,29 It is known that estradiol promotes endothelial cell activities.…”

Section: Discussionmentioning

confidence: 99%

Encouraging results in the treatment of haemorrhagic cystitis with estrogen – report of 10 cases and review of the literature

Ordemann

Naumann

Geissler

et al. 2000

Bone Marrow Transplant

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Summary:Haemorrhagic cystitis (HC) after allogeneic haematopoietic stem cell transplantation (HSCT) or high-dose cyclophosphamide (CP) chemotherapy is a severe sideeffect and can cause significant morbidity and mortality. In this report, we describe the clinical courses of 10 patients with HC and review the literature. The patients were treated with oral conjugated estrogen in an attempt to improve severe haemorrhagic cystitis. In seven patients positive effects were seen, haematuria resolved in all, but residual symptoms of dysuria remained for longer periods. In one patient application of estrogen was interrupted because of hepatotoxicity. Two patients failed all treatment modalities including oral estrogen because of terminal illness. We conclude that in the management of HC the administration of oral conjugated estrogen should be considered. Bone Marrow Transplantation (2000) 25, 981-985.

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“…In murine splenic macrophages, 17β-oestradiol down-regulates the LPS-induced production of IL-1α, IL-6 and TNF-α. However, the gene expression of IL-10, IL-12, and macrophage inflammatory protein was not affected (Deshpande et al, 1997). In our pilot study we validated real-time PCR for the quantification of immune cell response after LPS stimulation.…”

Section: Discussionmentioning

confidence: 94%

Preliminary results on intersexual differences in gene expression of chemokine K203 in mononuclear cells of chicken

Kankova

Zeman

Schwarz

et al. 2016

Acta Veterinaria Hungarica

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Sex steroid levels increase during sexual maturation and cause alterations in many physiological and morphological traits. Some of these changes may be connected with age-dependent and intersexual differences in the immune system. This topic is still insufficiently understood, especially in avian species, partially due to methodological limitations. In this study we measured the gene expression of proinflammatory cytokines (IL-1β, IL-6, IL-18) and chemokines [K60 (IL-8-like chicken chemokine -CXCLi1), CAF (IL-8-like chicken chemokine -CXCLi2), and K203] in mononuclear cells isolated from blood and spleen after in vitro stimulation with lipopolysaccharide (LPS). Samples were collected from chickens at two ages (from pullets before sexual maturity and from sexually mature egglaying hens). After LPS stimulation, a substantial increase was recorded in the gene expression of IL-6 and K203. All other measured genes were expressed at low levels in mononuclear cells irrespective of cell sources. We found a trend toward intersexual differences in K203 expression, but the expression of other cytokines and chemokines did not differ between the two sexes. The effect of stimulation was more pronounced in monocytes than in spleen macrophages, mainly in IL-6, IL-1β and K203 gene expression. Our findings represent a basis for further studies on the effects exerted by sexual hormones on the immune phenotype of birds.

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Estradiol Down‐Regulates LPS‐Induced Cytokine Production and NFkB Activation in Murine Macrophages

Abstract: Because cytokines are important mediators of immune function, the alteration of cytokine production by 17 beta-estradiol may thus have a profound effect on the outcome of immune response during inflammation.

Cited by 270 publications

References 52 publications

Sex differences and estrogen modulation of the cellular immune response after injury

Sex differences and estrogen modulation of the cellular immune response after injury

Encouraging results in the treatment of haemorrhagic cystitis with estrogen – report of 10 cases and review of the literature

Preliminary results on intersexual differences in gene expression of chemokine K203 in mononuclear cells of chicken

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