Estradiol improves tributyltin degradation by the filamentous fungus Metarhizium robertsii

Siewiera, Paulina; Bernat, Przemysław; Różalska, Sylwia; Długoński, Jerzy

doi:10.1016/j.ibiod.2015.06.014

Cited by 21 publications

(15 citation statements)

References 23 publications

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“…The ability of the microorganism to degrade organotins was confirmed in an earlier paper (Siewiera et al 2015). …”

Section: Methodssupporting

confidence: 58%

“…In this study, an attempt was undertaken to improve the process of dibutyltin biodegradation using a microscopic fungus Metarhizium robertsii. The selected strain can eliminate TBT with high levels of efficiency by protecting the fungal cells from oxidative stress through the application of 17β-estradiol (Siewiera et al 2015 ). In the present research, ascorbic acid (AA, vitamin C) and α-tocopherol (vitamin E) were chosen as primary antioxidants of the aqueous and lipophilic phases, respectively (Li and Schellhorn 2007 ).…”

Section: Introductionmentioning

confidence: 99%

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Efficient dibutyltin (DBT) elimination by the microscopic fungus Metarhizium robertsii under conditions of intensive aeration and ascorbic acid supplementation

Siewiera

Różalska

Bernat

2017

Environ Sci Pollut Res

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Dibutyltin (DBT) is an environmental pollutant characterized by immunotoxic, neurotoxic, and pro-oxidant properties. In this study, an attempt was made to enhance DBT elimination by the Metarhizium robertsii strain. We observed enhanced fungal growth in the bioreactor (pO2 ≥ 20%) compared to flask cultures (μ max increased from 0.061 to 0.086 h−1). Moreover, under aerated conditions, M. robertsii mycelium with “hairy” morphology biodegraded DBT (20 mg l−1) 10-fold faster in the bioreactor than in the flask cultures. Monobutyltin (MBT) and a hydroxylated derivative of MBT (OHBuSnH2) were detected as by-products of dibutyltin debutylation. Simultaneous usage of glucose and butyltins indicates the comatabolic nature of monobutyltin and dibutyltin removal. In order to protect fungal cells from oxidative stress caused by DBT presence, vitamin C (20 mg l−1) was applied. Supplementation with ascorbic acid (AA) resulted in a 3-fold acceleration of MBT removal during the first 7 h of incubation. Using the HPLC-MS/MS technique, a quantitative analysis of malondialdehyde (MDA), a marker of oxidative stress, was performed. In the AA presence, a decrease in the MDA amount (about 45%) was observed compared to the case with fungal cells exposed to DBT alone.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-017-8764-4) contains supplementary material, which is available to authorized users.

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“…The ability of the microorganism to degrade organotins was confirmed in an earlier paper (Siewiera et al 2015). …”

Section: Methodssupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Efficient dibutyltin (DBT) elimination by the microscopic fungus Metarhizium robertsii under conditions of intensive aeration and ascorbic acid supplementation

Siewiera

Różalska

Bernat

2017

Environ Sci Pollut Res

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“…The intention was to check whether acetamiprid simply interferes with the transport of dtxs from the fungal cell to the culture medium, or its action is connected with another mechanism. The procedure was performed according to the method described by Siewiera et al, 2015 [ 30 ] with some modifications. Briefly, 1 mL each of the control samples and the tested samples with acetamiprid concentrations of 5, 25 and 50 mg L −1 was transferred into Eppendorf tubes and then centrifuged for 10 min at 12,000 rpm.…”

Section: Methodsmentioning

confidence: 99%

Acetamiprid Affects Destruxins Production but Its Accumulation in Metarhizium sp. Spores Increases Infection Ability of Fungi

Nowak

Bernat

Mrozińska

et al. 2020

Toxins

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Metarhizium sp. are entomopathogenic fungi that inhabit the soil environment. Together, they act as natural pest control factors. In the natural environment, they come into contact with various anthropogenic pollutants, and sometimes, they are used together and interchangeably with chemical insecticides (e.g., neonicotinoids) for pest control. In most cases, the compatibility of entomopathogens with insecticides has been determined; however, the influence of these compounds on the metabolism of entomopathogenic fungi has not yet been studied. Secondary metabolites are very important factors that influence the fitness of the producers, playing important roles in the ability of these pathogens to successfully parasitize insects. In this study, for the first time, we focus on whether the insecticide present in the fungal growth environment affects secondary metabolism in fungi. The research revealed that acetamiprid at concentrations from 5 to 50 mg L−1 did not inhibit the growth of all tested Metarhizium sp.; however, it reduced the level of 19 produced destruxins in direct proportion to the dosage used. Furthermore, it was shown that acetamiprid accumulates not only in plant or animal tissues, but also in fungal cells. Despite the negative impact of acetamiprid on secondary metabolism, it was proofed to accumulate in Metarhizium spores, which appeared to have a stronger infectious potential against mealworm Tenebrio molitor, in comparison to the insecticide or the biological agent alone.

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“…The permeability of the fungal membranes was examined according to the method described by Siewiera et al [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader

et al. 2018

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The study reports the response to herbicide of the 2,4-dichlorophenoxyacetic acid (2,4-D)–degrading fungal strain Umbelopsis isabellina. A comparative analysis covered 41 free amino acids as well as 140 lipid species of fatty acids, phospholipids, acylglycerols, sphingolipids, and sterols. 2,4-D presence led to a decrease in fungal catalase activity, associated with a higher amount of thiobarbituric acid-reactive substances (TBARS). Damage to cells treated with the herbicide resulted in increased membrane permeability and decreased membrane fluidity. Detailed lipidomic profiling showed changes in the fatty acids composition such as an increase in the level of linoleic acid (C18:2). Moreover, an increase in the phosphatidylethanolamine/phosphatidylcholine ratio was observed. Analysis of fungal lipid profiles revealed that the presence of 2,4-D was accompanied by the accumulation of triacylglycerols, a decrease in ergosterol content, and a considerable rise in the level of sphingolipid ceramides. In the exponential phase of growth, increased levels of leucine, glycine, serine, asparagine, and hydroxyproline were found. The results obtained in our study confirmed that in the cultures of U. isabellina oxidative stress was caused by 2,4-D. The herbicide itself forced changes not only to membrane lipids but also to neutral lipids and amino acids, as the difference of tested compounds profiles between 2,4-D—containing and control samples was consequently lower as the pesticide degradation progressed. The presented findings may have a significant impact on the basic understanding of 2,4-D biodegradation and may be applied for process optimization on metabolomic and lipidomic levels.

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Estradiol improves tributyltin degradation by the filamentous fungus Metarhizium robertsii

Cited by 21 publications

References 23 publications

Efficient dibutyltin (DBT) elimination by the microscopic fungus Metarhizium robertsii under conditions of intensive aeration and ascorbic acid supplementation

Efficient dibutyltin (DBT) elimination by the microscopic fungus Metarhizium robertsii under conditions of intensive aeration and ascorbic acid supplementation

Acetamiprid Affects Destruxins Production but Its Accumulation in Metarhizium sp. Spores Increases Infection Ability of Fungi

2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader

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