Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method

Verdonk, Sara J. E.; Vesper, Hubert W.; Martens, Frans; Sluss, Patrick M.; Hillebrand, J.J.G.; Heijboer, Annemieke C.

doi:10.1016/j.cca.2019.04.062

Cited by 52 publications

(46 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This pattern is in accordance with previous reports using immunoassays (46); however, absolute concentrations may differ. Importantly, our present results are in accordance with reference ranges from the only existing paper on E2 determined by LC-MS/MS across the menstrual cycle (47). This study exhibits the limitation that we did not collect daily blood samples throughout the menstrual cycle but had to group subjects into early/late proliferative phase and luteal phase, as we only had one blood sample for each woman.…”

Section: Menstrual Cycle and Oral Contraceptive Usesupporting

confidence: 89%

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Frederiksen¹,

Th²,

Andersen³

et al. 2020

ESPE

View full text Add to dashboard Cite

The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations. Objective: To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3). Design: LC-MS/MS method development and construction of estrogen reference ranges. Settings: Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas. Participants: Healthy participants aged 3 months to 61 years (n = 1838). Results: An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2, and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged from

show abstract

Section: Menstrual Cycle and Oral Contraceptive Usesupporting

confidence: 89%

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Frederiksen¹,

Th²,

Andersen³

et al. 2020

ESPE

View full text Add to dashboard Cite

show abstract

“…Albumin was measured to calculate free and non-SHBG bound testosterone (free + albumin bound testosterone, according to Vermeulen). [ 18 , 19 ] Reference values were established in house; estradiol: <26 pmol/L in postmenopausal women and 12 to 136 pmol/L in men [ 17 ] ; total testosterone: 0.3 to 1.6 nmol/L in postmenopausal women, and 9 to 30 nmol/L in men [ 19 ] ; free testosterone: 2.3 to 26 pmol/L in postmenopausal women, 165 to 655 pmol/L in men [ 19 ] ; SHBG: 18 to 114 nmol/L in postmenopausal women and 13 to 71 nmol/L in men. Concentrations below or above the limits were defined as low and high, respectively.…”

Section: Methodsmentioning

confidence: 99%

Sex steroid hormones are associated with mortality in COVID-19 patients

et al. 2021

Self Cite

View full text Add to dashboard Cite

In patients with coronavirus disease 2019 (COVID-19), men are more severely affected than women. Multiple studies suggest that androgens might play a role in this difference in disease severity. Our objective was to assess the association between sex hormone levels and mortality in patients with severe COVID-19. We selected patients from the Amsterdam University Medical Centers COVID-19 Biobank, in which patients admitted to hospital in March and April 2020, with reverse transcription-polymerase chain reaction proven severe acute respiratory syndrome-coronavirus-2 infection, were prospectively included. Specifically, we included postmenopausal women (>55 years) and age-matched men, with a mortality of 50% in each group. Residual plasma samples were used to measure testosterone, estradiol, sex hormone binding globulin (SHBG), and albumin. We investigated the association of the levels of these hormones with mortality in men and women. We included 16 women and 24 men in March and April 2020 of whom 7 (44%) and 13 (54%), respectively, died. Median age was 69 years (interquartile range [IQR] 64–75). In men, both total and free testosterone was significantly lower in deceased patients (median testosterone 0.8 nmol/L [IQR 0.4–1.9] in deceased patients vs 3.2 nmol/L [IQR 2.1–7.5] in survivors; P < .001, and median free testosterone 33.2 pmol/L [IQR 15.3–52.2] in deceased patients vs 90.3 pmol/L [IQR 49.1–209.7] in survivors; P = .002). SHBG levels were significantly lower in both men and women who died (18.5 nmol/L [IQR 11.3–24.3] in deceased patients vs 34.0 nmol/L [IQR 25.0–48.0] in survivors; P < .001). No difference in estradiol levels was found between deceased and surviving patients. Low SHBG levels were associated with mortality rate in patients with COVID-19, and low total and free testosterone levels were associated with mortality in men. The role of testosterone and SHBG and potential of hormone replacement therapy needs further exploration in COVID-19.

show abstract

“…All samples were analyzed at the Endocrine Laboratory of Amsterdam UMC. DHEA-S, testosterone, androstenedione and estradiol (in males only) were measured in plasma using isotope diluted liquid chromatography mass spectrometry (ID-LC-MS/MS) [19][20][21] . Sex hormonebinding globulin (SHBG) was measured using an automated immunoassay (Architect, Abbott Diagnostics).…”

Section: Sex Hormone Analysismentioning

confidence: 99%

Interplay of sex hormones and long-term right ventricular adaptation in a Dutch PAH-cohort

Wezenbeek

Groeneveldt

Llucià‐Valldeperas

et al. 2022

The Journal of Heart and Lung Transplantation

Self Cite

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method

Cited by 52 publications

References 21 publications

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Sex steroid hormones are associated with mortality in COVID-19 patients

Interplay of sex hormones and long-term right ventricular adaptation in a Dutch PAH-cohort

Contact Info

Product

Resources

About

Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method

Cited by 52 publications

References 21 publications

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Sex steroid hormones are associated with mortality in COVID-19 patients

Interplay of sex hormones and long-term right ventricular adaptation in a Dutch PAH-cohort

Contact Info

Product

Resources

About

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LCMS/MS