Estragole prevents gastric ulcers via cytoprotective, antioxidant and immunoregulatory mechanisms in animal models

Júnior, Edvaldo Balbino Alves; Formiga, Rodrigo de Oliveira; Serafim, Catarina; Araruna, Maria Elaine Cristina; Pessoa, Michele Liz de Souza; Vasconcelos, Roseane Carvalho; Carvalho, Thaís Gomes de; Jesus, Tamires Gonçalves de; Araújo, Aurigena Antunes de; Araújo, Raimundo Fernandes de; Vieira, Giciane Carvalho; Sobral, Marianna Vieira; Batista, Leônia Maria

doi:10.1016/j.biopha.2020.110578

Cited by 29 publications

(9 citation statements)

References 64 publications

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“…Our findings showed that the administration of LCS-SENPs increased the enzyme activity of serum and colonic GSH-Px and reduced colonic MDA production. Previous studies suggested that GSH-Px plays an important role in protecting the colon against oxidative damage [ 66 , 67 ]. Oxidative stress or cell damage is caused by free radicals and usually involves lipid peroxidation [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice

Peng

Jiao

et al. 2022

IJMS

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Selenium nanoparticles have attracted extensive attention due to their good bioavailability and activity. In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid. The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements. The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated. The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared. LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se. LCS-SeNPs reduced colon injury and inflammation symptoms and improved intestinal barrier dysfunction. LCS-SeNPs significantly reduced serum and colonic inflammatory cytokines TNF-α and IL-6 levels. Moreover, LCS-SeNPs remarkably increased antioxidant enzyme GSH-Px levels in serum and colonic tissue. Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway. Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.

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Section: Discussionmentioning

confidence: 99%

Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice

Peng

Jiao

et al. 2022

IJMS

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“…In the case of commercial Hr_EO, the GC-MS analysis revealed the presence of 13 compounds (Table 1), among which estragole represented 63.1% from the total oil composition and could be associated with the biological effects exerted by Hr_EO. Estragole is a representative of the alkenylbenzenes class [24], exerting cytoprotective, antioxidant, and anti-inflammatory properties on gastric cells [25], and pro-apoptotic effects on HepG2 hepatocarcinoma cells [24]. In the realm of colorectal cancer, this phytocompound has not been studied so far.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis and In Vitro Cytotoxic Activity against Colorectal Adenocarcinoma Cells of Hippophae rhamnodies L., Cymbopogon citratus (D.C.) Stapf, and Ocimum basilicum L. Essential Oils

Dolghi

Buzatu

Dobrescu

et al. 2021

Plants

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Colorectal carcinoma (CRC) is one of the most frequently diagnosed cancer types with current deficient and aggressive treatment options, but various studied alternative therapies are able to efficiently contribute to its management. Essential oils (EOs) contain valuable compounds, with antibacterial, anti-inflammatory, and anticancer properties, which might serve as effective solutions in CRC prophylaxis or treatment. The aim of the present work was to evaluate the phytochemical composition and in vitro biological activity of essential oils derived from Hippophae rhamnoides (Hr_EO), Cymbopogon citratus (Cc_EO), and Ocimum basilicum (Ob_EO) species on HT-29 and Caco-2 human colorectal adenocarcinoma cell lines. The main compounds identified by GC-MS analysis were estragole (Hr_EO, Ob_EO), alpha- and beta-citral (Cc_EO). All tested EOs exerted a dose-dependent cytotoxicity on both cell lines by reducing the cell viability, especially in the case of Cc_EO, where at 75 µg/mL the viability percentages reached the values of 62.69% (Caco-2) and 64.09% (HT-29), respectively. The nuclear morphology evaluation highlighted significant dysmorphologies on both lines after their treatment with EOs at 75 µg/mL.

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“…An external test set was curated by searching PubMed for papers from the last two years 2022–2020 describing “mouse and LD 50 .” These papers resulted in 53 molecules not in the training sets (Table ) that were ultimately used as a test set for the mouse toxicity models consisting of mouse data for oral (46), IV (4), and IP (2). − …”

Section: Methodsmentioning

confidence: 99%

Comparing LD₅₀/LC₅₀ Machine Learning Models for Multiple Species

Lane

Harris

Urbina

et al. 2023

ACS Chem. Health Saf.

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The lethal dose or concentration which kills 50% of the animals (LD50 or LC50) is an important parameter for scientists to understand the toxicity of chemicals in different scenarios that can be used to make go-no-go decisions, and ultimately assist in the choice of the right personal protective equipment needed for containment. The LD50 assessment process has also required the use of many animals although modern methods have reduced the number of rats needed. Since a compound is usually considered highly toxic when the LD50 is lower than 25 mg/kg, such a classification provides potentially valuable safety information to synthetic chemists and other safety assessment scientists. The need for finding alternative approaches such as computational methods is important to ultimately reduce animal use for this testing further still. We now summarize our efforts to use public data for building in vivo LD50 or LC50 classification and regression machine learning models for various species (rat, mouse, fish, and daphnia) and their fivefold cross-validation statistics with different machine learning algorithms as well as an external curated test set for mouse LD50. These datasets consist of different molecule classes, may cover different activity ranges, and also have a range of dataset sizes. The challenges of using such computational models are that their applicability domain will also need to be understood so that they can be used to make reliable predictions for novel molecules. These machine learning models will also need to be backed up with experimental validation. However, such models could also be used for efforts to bridge gaps in individual toxicity datasets. Making such models available also opens them up to potential misuse or dual use. We will summarize these efforts and propose that they could be used for scoring the millions of commercially available molecules, most of which likely do not have a known LD50 or for that matter any data in vitro or in vivo for toxicity.

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Estragole prevents gastric ulcers via cytoprotective, antioxidant and immunoregulatory mechanisms in animal models

Cited by 29 publications

References 64 publications

Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice

Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice

Phytochemical Analysis and In Vitro Cytotoxic Activity against Colorectal Adenocarcinoma Cells of Hippophae rhamnodies L., Cymbopogon citratus (D.C.) Stapf, and Ocimum basilicum L. Essential Oils

Comparing LD₅₀/LC₅₀ Machine Learning Models for Multiple Species

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Estragole prevents gastric ulcers via cytoprotective, antioxidant and immunoregulatory mechanisms in animal models

Cited by 29 publications

References 64 publications

Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice

Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice

Phytochemical Analysis and In Vitro Cytotoxic Activity against Colorectal Adenocarcinoma Cells of Hippophae rhamnodies L., Cymbopogon citratus (D.C.) Stapf, and Ocimum basilicum L. Essential Oils

Comparing LD50/LC50 Machine Learning Models for Multiple Species

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Comparing LD₅₀/LC₅₀ Machine Learning Models for Multiple Species