Estrogen and progesterone receptors and antioxidant enzymes are expressed differently in the uterus of domestic cats during the estrous cycle

Nascimento, Acácia Eduarda de Jesus; Santos, Luciano Cardoso; Santos, Bianca Reis; Santos, Emilly Oliveira; Cunha, Maria Clara da Silva Galrão; Snoeck, Paola Pereira das Neves; Lavor, Mário Sérgio Lima de; Silva, Juneo Freitas

doi:10.1016/j.theriogenology.2023.03.007

Cited by 4 publications

(4 citation statements)

References 55 publications

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“…12 It is plausible that this increase in GPX1 in the uterus of cats with pyometra may be associated with an increase in endometrial ERα and PR, since we observed a physiological reduction of Gpx1 in the endometrium of cats in diestrus, concomitant with the reduction of ERα and PR. 34 Furthermore, a positive correlation was observed between plasma E2 and uterine levels of GPX in women, 38 like that observed in uterine GPX expression in rats treated with E2, 39 in agreement with the results of the present study. The increase in GPX1 in the uterus of cats with pyometra may also reflect increased uterine oxidative stress observed in these animals, as the body increases the expression of antioxidant enzymes in order to control the increase in reactive oxygen species released by neutrophils and macrophages in the endometrium.…”

Section: Discussionsupporting

confidence: 91%

“…33 Interestingly, the reduction in uterine expression of catalase in cats with pyometra was accom panied by an increase in gene and protein expression of ERα and PR, contrary to what occurs physiologically in the uterus of cats in diestrus. 34 In addition, previous studies in rats demonstrated that the administration of E 2 increased catalase expression in the uterus, while ova riectomy reduced its expression. 35,36 This differs from the present study, in which cats with pyometra showed reduced uterine expression of catalase, even with high plasma levels of E 2 and P 4 and higher endometrial expres sion of ERα.…”

Section: Discussionmentioning

confidence: 96%

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Pyometra alters the redox status and expression of estrogen and progesterone receptors in the uterus of domestic cats

Nascimento,

Santos,

Santos

et al. 2023

Journal of Feline Medicine and Surgery

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Objectives The aim of this study was to evaluate the expression profile of sex steroid receptors and redox mediators in the uterus of domestic cats with pyometra. Methods Twelve cats were used and divided into groups: (1) non-gestational healthy diestrus (n = 7) and (2) pyometra (n = 5). The plasma profiles of estradiol and progesterone (P4) as well as uterine expression levels of estradiol alpha (ERα), progesterone (PR) and androgen (AR) receptors, of the antioxidant enzymes superoxide dismutase 1 (SOD1), catalase and glutathione peroxidase 1 (GPX1), and of the oxidative damage marker 8-hydroxy-2′-deoxyguanosine (8-OHdG) were evaluated. Results Cats with pyometra showed higher plasma P4 levels and increased uterine messenger RNA (mRNA) and protein expression of ERα and PR, mainly in the glandular epithelium for ERα and in stromal and myometrial cells for PR. In addition, there was an increase in 8-OHdG immunostaining and GPX1 mRNA and protein expression in cats with pyometra compared with those in non-gestational diestrus, while catalase showed a reduction in endometrial immunostaining in cats with pyometra. There were no differences in uterine AR and SOD1 expression between the groups. Conclusion and relevance The findings of this study showed that pyometra is associated with oxidative stress in the uterus of domestic cats and alterations of the profile of sex steroid receptors, especially ERα and PR, and of antioxidant enzymes, suggesting that changes in these mediators may play a role with the etiopathogenesis of this disease.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 96%

Pyometra alters the redox status and expression of estrogen and progesterone receptors in the uterus of domestic cats

Nascimento,

Santos,

Santos

et al. 2023

Journal of Feline Medicine and Surgery

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“…For ERα, in turn, low cell positivity scores in the different treatment groups were observed, perhaps associated with the low expression of this receptor in the dioestrous phase, as observed in other studies in bitches (Kunkitti et al, 2011;Marinković et al, 2018;Ozyurtlu et al, 2010) and queens (de Jesus Nascimento et al, 2023).…”

Section: Discussionsupporting

confidence: 70%

“…Regarding AR, its labelling was weak or null in the regions of luminal epithelium and deep glands, while in the stroma and myometrium, immunostaining was weak to moderate. Studies have already shown a similar pattern of expression in the dioestrous phase, which did not differ in relation to other phases of the oestrous cycle in bitches (Sauerwein et al., 1998; Vermeirsch et al., 2002), queens (de Jesus Nascimento et al., 2023), and pigs (Cárdenas & Pope, 2003). In sheep, higher protein density was associated with the oestrous phase, and lower mRNA levels in the proestrous phase in relation to other phases of the oestrous cycle (Duan et al., 2019).…”

Section: Discussionmentioning

confidence: 73%

Modulatory effect of vitamin B6 on sex hormone receptors, thyroid, and kisspeptin/kiss1r system in the uterus of pseudopregnant bitches

Santos,

Santana,

Niella

et al. 2024

Reprod Domestic Animals

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This study evaluated whether the treatment of pseudopregnancy in bitches with vitamin B6 modulates uterine expression of receptors for progesterone (PR), oestrogen (ERα), androgen (AR), thyroid hormone (TRα) and the kisspeptin/Kiss1r system. Eighteen pseudopregnant bitches were treated for 20 days in groups receiving placebo (n = 6); cabergoline (5 μg/kg/day; n = 6); or vitamin B6 (50 mg/kg/day; n = 6). Blood was collected on the 1st day of drug administration and 120 h later to measure serum prolactin (PRL). After treatment, they were ovariohysterectomized and uterine fragments were collected for histomorphometry and immunohistochemical evaluation of PR, ERα, AR, TRα, Kiss1 and Kiss1r. After 120 h of cabergoline or vitamin B6 treatment, PRL levels were reduced in the bitches, confirming the antiprolactinemic effect of these drugs. Furthermore, regardless of treatment, the animals exhibited uterine histomorphometry consistent with dioestrus. The PR showed strong immunostaining in all regions and an increase in scores was observed for this receptor in animals treated with vitamin B6 in deep glands. In contrast, ERα and Kiss1R receptors showed weak to no immunostaining in all uterine regions and no changes between groups. Regarding AR, most animals treated with vitamin B6 showed increased trends in the deep gland and myometrium marking scores. In contrast, in both vitamin B6 and cabergoline treatments, a reduction in TRα marking scores was observed compared to the control group. In addition, on the endometrial surface, a reduction was observed in the marked area of Kiss1 after administration of cabergoline when compared to the pseudopregnant control group. These findings shed valuable insight into the use of vitamin B6 as a drug with actions similar to cabergoline in reducing PRL and uterine modulation in bitches.

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Molecular Factors Involved in the Reproductive Morphophysiology of Female Domestic Cat (Felis catus)

Santos,

Silva

2023

Animals

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The domestic cat (Felis catus) is considered an important model for the study of feline reproductive morphophysiology. However, although the morphological changes and clinical signs that occur during the estrous cycle and pregnancy are well known, little is known about the molecular mechanisms involved in the reproductive physiology of this animal species. Thus, this paper reviews the current knowledge about the modulation and expression profile of hormonal, immunological, redox, and growth mediators involved in the uterine, ovarian, and placental morphophysiology of domestic cats.

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Estrogen and progesterone receptors and antioxidant enzymes are expressed differently in the uterus of domestic cats during the estrous cycle

Cited by 4 publications

References 55 publications

Pyometra alters the redox status and expression of estrogen and progesterone receptors in the uterus of domestic cats

Pyometra alters the redox status and expression of estrogen and progesterone receptors in the uterus of domestic cats

Modulatory effect of vitamin B6 on sex hormone receptors, thyroid, and kisspeptin/kiss1r system in the uterus of pseudopregnant bitches

Molecular Factors Involved in the Reproductive Morphophysiology of Female Domestic Cat (Felis catus)

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