Estrogen and progestogen receptors in rat uterus at the time of implantation

Hertogh, R. De; Ekka, E.; Vanderheyden, I.; Glorieux, B.

doi:10.1016/0022-4731(86)90570-4

Cited by 2 publications

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“…In this regard, it is unclear whether metabolism of ECM is regulated hormonally in neonatal rodents, since the neonatal ovary is endocrinologically inactive (Csernus, 1986) and since maternal estrogen present at birth is largely bound to alphafetoprotein (Henry and Miller, 1986;Raynaud, 1973). In mature uteri, the endometrial stroma and myometrium have estrogen and progesterone receptors (Bayard et al, 1978;Stumpf and Sar, 1976;McCormack and Glasser, 1980;McClellan et al, 1986;Scharl et al, 1988;West et al, 1986West et al, , 1987Sadan et al, 1987;De Hertogh et al, 1986). These sex-hormone receptors are thought to be involved in regulation of the changes in extracellular matrix proteins during decidualization and following parturition (Wewer et al, 1986;Salvador and Tsai, 1973).…”

Section: Discussionmentioning

confidence: 97%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Brody

Cunha

1989

Am. J. Anat.

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The effects of diethylstilbestrol (DES) treatment on myometrial development from the prenatal to adult period were examined in rats and mice by histologic and immunocytochemical methods using anti-actin, -vimentin, and -laminin to assess cytodifferentiation of smooth muscle and fibroblastic cells, and by morphometric procedures to assess quantitatively the effect of DES on the expression of cellular orientation in the emerging inner circular myometrial layer. Neonatal rats and mice were treated with DES from day 0 (day of birth) to day 2 with dosages known to perturb myometrial development. Neonatal treatment with DES increased the degree of circular orientation within the uterine mesenchyme, an effect detectable following as little as 24 hr of DES treatment. This effect on spatial organization of the mesenchyme was followed by an increase in the thickness of the actin-positive middle layer (prospective circular myometrium) of uterine mesenchyme during days 3-15; from day 15 onward, however, the circular myometrial layer began to fragment into irregular bundles of smooth muscle, and the longitudinal myometrial layer became thinner and more irregularly organized than controls. Vimentin localization in rats treated with DES neonatally was more intense than in controls within the circularly orientated uterine mesenchyme at 5 days. By 60 days the circular and longitudinal myometrial layers of DES-treated animals showed strands and bundles of vimentin-positive cells, which were not present in controls. Both rats and mice show comparable effects of DES treatment.

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Section: Discussionmentioning

confidence: 97%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Brody

Cunha

1989

Am. J. Anat.

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“…In outbred CD-1 mice, epithelial estrogen receptors have been detected as early as 3 days postpartum (Bigsby and Cunha, unpublished;Korach et al, 1988). Moreover, cells of the endometrial stroma and myometrium also have progesterone receptors Sadan et al, 1987;De Hertogh et al, 1986). These sex-hormone receptors are thought to be involved in the regulation of cellular proliferation in the endometrial stroma and myometrium (Martin and Finn, 1968;Tachi et al, 1972;Finn and Porter, 1975;Martin, 1980), in regulation of the changes in extracellular matrix proteins during decidualization and following parturition Salvador and Tsai, 1973), and in decidualization per se (Finn and Martin, 1972;DeFeo, 1967;Glasser and Clark, 1975).…”

Section: Introductionmentioning

confidence: 93%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: I. Normal development

Brody¹,

Cunha²

1989

Am. J. Anat.

140

115

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Myometrial development from the prenatal to adult period was examined in rats and mice 1) by histologic and immunocytochemical methods with anti-actin, -vimentin, and -laminin to assess cytodifferentiation of smooth muscle and fibroblastic cells; and 2) by morphometric procedures to assess quantitatively the expression of cellular orientation in the emerging inner circular myometrial layer. Uterine mesenchymal cells initially were uniformly vimentin-positive, undifferentiated, and randomly oriented during the late fetal period. By the early neonatal period, three mesenchymal layers became recognizable histologically, the middle one of which (prospective circular myometrium) developed distinct circular orientation and differentiated into a layer composed of actin-positive smooth muscle cells. The cells of the inner mesenchymal layer initially exhibited radial orientation. By 10 days postpartum, the outer longitudinal mesenchymal layer differentiated into bundles of smooth muscle cells representing the longitudinal myometrium. The inner mesenchymal layer remained vimentin-positive and differentiated into the randomly ordered endometrial stroma. The cells of the middle and outer mesenchymal layers that were destined to form myometrium initially expressed vimentin throughout and then coexpressed vimentin and actin, but with time vimentin staining disappeared in the maturing smooth muscle cells as they expressed actin.

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Estrogen and progestogen receptors in rat uterus at the time of implantation

Cited by 2 publications

References 0 publications

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: I. Normal development

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