Estrogen blocks homocysteine-induced endothelial dysfunction in porcine coronary arteries1,2

Spencer, Todd A.; Chai, Hong; Fu, Weiping; Ramaswami, Ganesh; Cox, Margueritte; Conklin, Brian S.; Lin, Peter H.; Lumsden, Alan B.; Yao, Qizhi; Chen, Changyi

doi:10.1016/j.jss.2004.01.021

Cited by 20 publications

(28 citation statements)

References 31 publications

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“…In cell culture model, Studies have shown that Rb1 blocks the calcium channel and inhibits free radical in ventricular myocardiocytes of Wistar rats [26], both of which play important role in protecting heart from ischemic and reperfusion injury. In vitro, ginsenoside Rb1, similar to red wine and estrogen, has been shown to reduce homocysteine-induced endothelial dysfunction and free radical production as well as eNOS downregulation in porcine coronary arteries [27][28][29]. Kwan et al also showed that P. ginseng extracts induced endothelium-dependent vasorelaxation through a mechanism of activation of the eNOS system in both dog and rat arteries [30].…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 Preconditioning Protects Against Myocardial Infarction After Regional Ischemia and Reperfusion by Activation of Phosphatidylinositol-3-kinase Signal Transduction

Zhi

et al. 2008

Cardiovasc Drugs Ther

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Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 Preconditioning Protects Against Myocardial Infarction After Regional Ischemia and Reperfusion by Activation of Phosphatidylinositol-3-kinase Signal Transduction

Zhi

et al. 2008

Cardiovasc Drugs Ther

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“…The myograph system used in our laboratory has been previously described (5,12,23,28). Briefly, the rings were cultured for 24 h and suspended between the wires of the organ bath myograph chamber (Danish Myo Technology Organ Bath 700 MO, Aarhus, Denmark) in 6 ml of Krebs solution, maintained at 37°C, and oxygenated with pure oxygen gas.…”

Section: Methodsmentioning

confidence: 99%

“…eNOS immunohistochemistry. Immunohistochemistry staining procedure was previously described (5,12,23,28). Briefly, the paraffin section of porcine coronary artery rings was incubated with monoclonal antibody against human eNOS antibody (1:1,000) overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

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Ginsenosides block HIV protease inhibitor ritonavir-induced vascular dysfunction of porcine coronary arteries

Chai¹,

Wei²,

Lin³

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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Ginsenosides have been shown to have potential benefits on the cardiovascular system through diverse mechanisms, including antioxidative property. The objective of this study was to determine whether ginsenosides could prevent coronary arteries from RTVinduced dysfunction. Porcine coronary artery rings were incubated with RTV and ginsenosides Rb1, Rc, and Re for 24 h. Vasomotor function was recorded by a myograph tension system. In response to the thromboxane A2 analog U-46619, the contraction of the vessel rings was significantly reduced. When cocultured with Rb1, Rc, and Re, the contractility significantly increased. In response to bradykinin at 10 Ϫ5 M, the endothelium-dependent relaxation of vessel rings was significantly reduced by 59% for RTV compared with controls (P Ͻ 0.05). When cocultured with Rb1, Rc, and Re, the relaxation significantly increased 100%, 90%, and 134%, respectively, compared with the RTV-alone groups (P Ͼ 0.05). In response to sodium nitroprusside, RTV significantly reduced vasorelaxation. In addition, the endothelial nitric oxide synthase (eNOS) mRNA levels were significantly reduced by 78% for RTV group (P Ͻ 0.05) by real-time PCR analysis. The eNOS protein levels measured by Western blot analysis and nitrite concentrations measured by Griess assay were also decreased, whereas O 2 Ϫ production by lucigenin-enhanced chemiluminescence was significantly increased in the RTV-treated group. These effects of RTV were effectively blocked by ginsenosides. Thus HIV protease inhibitor RTV significantly impaired the vasomotor function of porcine coronary arteries. This effect may be mediated by the downregulation of eNOS and overproduction of O 2 Ϫ . These results suggest that ginsenosides can effectively block RTV-induced vascular dysfunction. oxidative stress; ginseng root; vasomotor; endothelial nitric oxide; superoxide anion; human immunodeficiency virus

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“…Hcy>12 µ mol/dl has been shown as a risk factor for vascular disease, brain athrophy and neurodegenrative disease [6]. Hyper-Homocysteinemia has been linked to atherosclerosis and thrombosis [7].…”

Section: Introductionmentioning

confidence: 99%

Exposure to Acute and Chronic Ethanol in Developmental Stage of Chick can Change the Brain Homocysteine and Leptin

Farahani¹,

Taherianfard²,

Nazifi³

2013

Biochem Physiol

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Aim: Ethanol intake decreases food intake in rat, and one of the possible mediators of this alcohol effect is leptin. On the other hand, the concentration of total plasma homocysteine is a well-established indicator for the risk of cardiovascular disease, and seems to be related to ethanol consumption. So, the aim of the present study was to investigate the effect of exposure to acute (70%) and chronic (10%) evaporated ethanol on 1) brain leptin and homocysteine concentration on the 15 th day of embryonic development of chick; 2) brain leptin and homocysteine concentration immediately after hatch of chick; 3) serum leptin concentration immediately after hatch of chick.Methods: 60 fertilized eggs were used. Eggs were divided into 3 groups, 1) Control; 2) acute exposure to ethanol 3) chronic exposure to ethanol. Homocysteine was measured by using enzyme-linked assay, and leptin was measured with the chick leptin radioimmunoassay kit.Results: Data showed that exposure to acute and chronic ethanol significantly (P<0.05) decreased brain homocysteine concentration on the 15 th day of embryonic stage of chicken, but did not have any effect on brain homocysteine concentration immediately after hatch Exposure to acute and chronic ethanol significantly (P<0.05) increased brain leptin on the 15 th day of embryonic stage, brain leptin immediately after hatch of chick and plasma leptin immediately after hatch of chick. Conclusion:Present results indicated that exposure to acute and chronic ethanol by evaporation in embryonic stage of chicken can change the brain homocysteine, brain leptin and serum leptin.

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Estrogen blocks homocysteine-induced endothelial dysfunction in porcine coronary arteries1,2

Cited by 20 publications

References 31 publications

Ginsenoside Rb1 Preconditioning Protects Against Myocardial Infarction After Regional Ischemia and Reperfusion by Activation of Phosphatidylinositol-3-kinase Signal Transduction

Ginsenoside Rb1 Preconditioning Protects Against Myocardial Infarction After Regional Ischemia and Reperfusion by Activation of Phosphatidylinositol-3-kinase Signal Transduction

Ginsenosides block HIV protease inhibitor ritonavir-induced vascular dysfunction of porcine coronary arteries

Exposure to Acute and Chronic Ethanol in Developmental Stage of Chick can Change the Brain Homocysteine and Leptin

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