Estrogen-Induced Differentiation of the Oviductal Epithelium in Prepubertal Dogs

Verhage, H. C.; Abel, John H.; Tietz, William J.; Barrau, Marny D.

doi:10.1093/biolreprod/9.5.475

Cited by 28 publications

(21 citation statements)

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“…Several studies have shown that the epithelial lining of the fimbria, ampulla, and isthmus is sensitive to endogenous and exogenous steroid hormone levels and that the morphological alterations vary depending upon the region of the oviduct [5,[15][16][17][18][19][20][21][22]24]. In the primate [15,[18][19][20] and carnivore [16,22,24], the process of ciliogenesis is dependent upon E 2 .…”

Section: Discussionmentioning

confidence: 99%

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Epithelial Lining of the Sheep Ampulla Oviduct Undergoes Pregnancy-Associated Morphological Changes in Secretory Status and Cell Height1

Murray

1995

Biology of Reproduction

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The biosynthetic activity of the sheep ampulla oviduct changes during the first few days of pregnancy. An estrogen (E2)-dependent glycoprotein (M(r) 90,000-92,000) is expressed, synthesized, and released when fertilization and embryo development occur in the oviduct. The objective of the present study was to determine whether the secretory epithelial lining of the ampulla, the cellular source of the E2-dependent protein, undergoes morphological alterations during early pregnancy is a contemporaneous manner. Ampulla oviducts obtained from ovariectomized (ovx), estrous (Day 0), and pregnant (Days 1.5, 2, 3, 4, 6, and 16) ewes were analyzed with light and electron microscopy. The mean cell height of epithelial cells from estrus and from Days 1.5, 2, and 3 of pregnancy was significantly (p < 0.05) higher than in ovx animals. A significant reduction in cell height was observed at Day 4 of pregnancy, and this value remained unchanged at Day 6 and Day 16. Ciliated and nonciliated cells were present in the ampulla at all stages examined, with ciliated cells constituting the major cell type. Consistent with previous results, the nonciliated cells obtained from ovx ewes were synthetically inactive and at estrus were characterized by well-developed secretory organelles, including abundant amounts of secretory granules containing the E2-dependent glycoprotein. Extensive microvilli and cytoplasmic blebs protruding into the oviduct lumen distinguished the apical surface of secretory epithelial cells until Day 3. At Day 1.5 the secretory cells contained dilated cisternae of rough endoplasmic reticulum and enlarged Golgi, which were progressively diminished with increasing stages of pregnancy. Exocytosis, fusion of secretory granules, and a reduction in their electron density were seen until Days 3-4. By Day 4 and Day 6, fewer granules were observed in the ampulla cytoplasm and few, if any, were present by Day 16. Increases in the amount of heterochromatin were seen in nuclei at Day 6 and Day 16. Extrusion of epithelial cells into the oviduct-lumen was evident at Day 16 of pregnancy. These data show that 1) the secretory epithelium of the sheep ampulla oviduct undergoes morphological alterations in protein-synthesizing organelles and apical specializations that vary with the stage of pregnancy, 2) the secretory product contained in lamellar granules is released by the process of exocytosis until Days 3-4, and 3) cell death appears to occur at Day 16 by shedding of epithelial cells into the oviduct lumen.

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Section: Discussionmentioning

confidence: 99%

“…In the primate [15,[18][19][20] and carnivore [16,22,24], the process of ciliogenesis is dependent upon E 2 . New cilia are formed in response to E 2 administration to ovx animals and during the estrous or follicular phase of the cycle.…”

Section: Discussionmentioning

confidence: 99%

Epithelial Lining of the Sheep Ampulla Oviduct Undergoes Pregnancy-Associated Morphological Changes in Secretory Status and Cell Height1

Murray

1995

Biology of Reproduction

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“…Atrophy and dedifferentiation, which includes deciliation, occur 'during metestrus when progesterone levels are high (201). In prepubertal dogs, the oviductal epi thelium is low, nonciliated, and nonsecretory; estrogen treatment stimulates the development of a fully differentiated epithelium (202).…”

Section: Hormonal Effects On the Oviductmentioning

confidence: 99%

Hormonal Regulation of the Reproductive Tract in Female Mammals

Brenner¹,

West²

1975

Annu. Rev. Physiol.

162

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INTRODUCTION +1131This review covers only a small part of what has recently become an enormous literature on hormonal regulation of the fe male reproductive tract. We used the fo llowing guidelines in selecting papers fo r review: 1. The reproductive tract is exposed to varying levels of sex steroids during the menstrual and estrous cycles. With the advent of radioimmunoassay techniques, these patterns are now known more precisely than at any previous time. 2. The steroids are concentrated in the target cells of the tract by mechanisms that. because of recent findings in steroid receptor interactions, are more fu lly understood than ever before. Here we pay particular attention to reports of fluctuations in receptor levels during reproductive cycles and pregnancy and to the correlation between such changes and the fluctuat ing levels of steroids in plasma. 3. Within the target cells, the steroids interact with the cell machinery to produce multiple effects. This is the frontier of the modern research effort on hormone actions; the potential fo r future success here is enor mous. So far, however, species and organ differences have thwarted attempts to define any universal principles of hormone action, although evidence is accumulat ing in favor of the idea that one of the key endpoints of steroid hormone action is the production of specific RNA molecules.As to our method of selecting reports to review, we have chosen those that illustrate the interactions between estrogens and progestins in regulating the growth and fu nction of the tract, that point to the complexity of these interactions, and that serve as guidelines fo r fu ture study. No attempt was made to be comprehensive. Some emphasis has been placed on the reproductive tract of primates because of our special interests. Further ANNUAL REVIEWS 274BRENNER & WEST Additional information is available in a recently published series of definitive reviews concerned with various aspects of endocrine control of the fe male reproduc tive system (66). These include the effects of steroid hormones on uterine metabo lism (20S); endocrine control of implantation (IS3); the overall role of hormones in pregnancy (76, 16 1); various aspects of the biosynthesis, metabolism, and uterine binding of estrogens (42, 61, 178, 18S); the biosynthesis, metabolism, and physiologi cal role of progesterone (3S, .59, 104, 14S); the effects of hormones on the anatomy and physiology of the cervix (63, 128); and the biological effects of oral contraceptive steroids (168). STEROID LEVELS DURING REPRODUCTIVE CYCLES Menstrual CyclesESTROGENS The cyclic pattern of estrogen secretion in nonhuman primates has been investigated in the rhesus monkey (11)(12)(13)(14)78, 8S, 86,100, IS7,207), cynomol gus monkeys (163), bonnet macaques (120), baboons (186), and chimpanzees (6S). It is generally agreed that in the rhesus monkey the level of estradiol (E2Pm peripheral plasma during the early fo llicular phase (days 1-8) is "'SO pg/ml, and that about 4 days before the luteinizing hormone (LH) peak there...

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“…While the proportion of ciliated cells gradually decreases from the ampulla to the isthmus, the secretory cells are well distributed throughout the oviductal ampulla in higher mammals (Fredericks, 1986;Hunter, 1988;Brenner and Maslar, 1988). During the ovarian cycle (Fredericks, 1986;Verhage et al, 1979;Verhage and Jaffe, 1986) or in response to exogenous estradiol (Verhage et al, 1973;Verhage and Brenner, 1975;Odor et al, 1980;Bareither and Verhage, 1981; Abe and Oikawa, 1993) the secretory cells undergo marked ultrastructural modifications associated with changes in secretory activities. While most proteins in the oviductal fluid are serum derived (Leese, 1988), some originate from the oviductal secretory cells.…”

mentioning

confidence: 99%

Oviductins possess chitinase‐ and mucin‐like domains: A lead in the search for the biological function of these oviduct‐specific ZP‐associating glycoproteins

Malette

Paquette

Merlen

et al. 1995

Molecular Reproduction Devel

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Over the last 10 years considerable progress has been made in the immunological and biochemical characterization of oviduct-specific glycoproteins. It is now well established that a subclass of these secretory products, designated as oviductins, associate with the zona pellucida of the ovulated oocyte and with the early embryo. Recent reports on the cloning of cDNAs of oviductins from various species, including that of golden hamster (Mesocricetus auratus) oviductin by our laboratory, allowed us to compare their deduced amino acid sequences with those of other proteins. Optimal alignment analysis showed that oviductins contain regions of significant similarity with catalytically inactive mammalian members of the bacterial and microfilarial chitinase protein family. Most importantly, a close examination of the hamster and human deduced amino acid sequences revealed that both glycoproteins possess contiguous Ser/Thr rich repeated units, clustered in their carboxy-terminal portions. These mucin-type motifs are similar in the hamster and human glycoprotein, although hamster oviductin contains more of these complete units. This striking feature might indicate that these molecules play a similar role to mucin-type glycoproteins, e.g., in protecting the oocyte and early embryo against attacks from their environment. W e propose a model whereby oviductins are targeted to the oocyte via the interaction of their chitinase-like domains with specific oligosaccharide moieties of the zona pellucida. Once localized to this structure, oviductin molecules would act as a protective shield around the oocyte and early embryo by virtue of their densely glycosylated mucin-type domains. o 1995 Wiley-Liss, Inc.

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Estrogen-Induced Differentiation of the Oviductal Epithelium in Prepubertal Dogs

Cited by 28 publications

References 0 publications

Epithelial Lining of the Sheep Ampulla Oviduct Undergoes Pregnancy-Associated Morphological Changes in Secretory Status and Cell Height1

Epithelial Lining of the Sheep Ampulla Oviduct Undergoes Pregnancy-Associated Morphological Changes in Secretory Status and Cell Height1

Hormonal Regulation of the Reproductive Tract in Female Mammals

Oviductins possess chitinase‐ and mucin‐like domains: A lead in the search for the biological function of these oviduct‐specific ZP‐associating glycoproteins

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